[논문]Immune Cells Activity, Cytotoxicity and Nitrite Scavenging Activity of Extracts from Several Resource Plants

Boo, Hee-Ock; Park, Jeong-Hun; Park, Hyeon-Yong

doi:10.7732/kjpr.2018.31.6.604

Immune Cells Activity, Cytotoxicity and Nitrite Scavenging Activity of Extracts from Several Resource Plants 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.31 no.6, 2018년, pp.604 - 611

Boo, Hee-Ock (Department of Biology, Chosun University) , Park, Jeong-Hun (Department of Biology, Chosun University) , Park, Hyeon-Yong (Department of Biology, Chosun University)

Abstract ▼ AI-Helper

This study was conducted to explore the immune activity, anticancer activity and nitrile scavenging activities of methanol extracts from the various organs of four Korean resource plants. The immune responses from both human T and B cell line was significantly enhanced in the cell growth compared to control while the cell growth was influenced at a certain period of culture. The results revealed that the cell growth of both human T and B cell was altered in a time dependent manner. Among tested several resource plants, the flower extract of E. japonicum demonstrated a pronounced cytotoxicity against HCT-116 cell with an IC50 value $132.08{\mu}g\;ml-1$. The flower extract from Corylopsis coreana had a promising scavenging activity against pH 1.2 compared to other species. Taken together, the studied resource plants have influenced significantly in response to immunity and also have the potential cytotoxicity and nitric scavenging activities. However, the species E. japonicum exhibited the pronounced activities from several resource plants. The result from this investigation suggests that the extracts of studied resource plant could be an addition to basic medicine for some diseases.

주제어

표/그림 (4)

그림 Fig. 1. The cell growth of human T cell line of each extract from several resource plants using hemocytometer.
그림 Fig. 2. The cell growth of human B cell line of each extract from several resource plants using hemocytometer.
표 Table 1. Cytotoxicity of extracts from the different organs of several resource plants on four human cancer cell lines
표 Table 2. Nitrite scavenging activities of the different organs extract in several resource plants

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대상 데이터

All experiments were conducted for three to five independent replicates. The statistical analysis was performed using the Statistical Analysis System software (SAS version 9.
In this experiment, four kinds of resource plant materials [Salicornia herbacea (aerial part), Corylopsis coreana (stem and flower), Erythronium japonicum (leaf, root and flower), Phragmites communis (root ad stem)] were used. These plants were chosen because of the possibility to obtain various physiological functionalities.

데이터처리

Means with the same letter in column are not significantly different at p<0.05 level by Duncan’s multiple range test.
The analysis of variance (ANOVA), followed by Duncan test was used to determine significant difference (p < 0.05) between the treatment means.

성능/효과

The cell growth of human T cell line of each extract from several resource plants is shown in Fig. 1. Compared to untreated cell (Control), the extracts from different organs (root, leaf and flower) of E. japonica, flower extract from C. coreana and stem extracts from P. communis showed higher immune enhancement effect. In contrast, the root extract from P.
The immune enhancement effect of human B cell line from several resource plants is shown in Fig. 2. The flower extract from C. coreana exhibited the highest immune activity on the 8^th day of cell culture. On the cell culture day 8, the all organs of the studied plants showed pronounced immune activity effects except the stem extract from P.
, 2010). Compared to other studied plants, the C. coreana exhibited the highest nitrite scavenging activity 96.4% and 91.5% respectively, and there was no distinct difference in the nitrite scavenging effects of flower and stem organs in the present study. In conclusion, the results obtained in the present study demonstrated the immune responses of the aerial parts of four resource plant related to its cytotoxic activities against cancer cell lines combined with nitrite scavenging activity.
Our present result demonstrated that extract from various organs from four resource plants exhibited a promising cytotoxic activity against all human cell lines at relatively higher concentration. In all extracts investigated, the lowest IC₅₀ value was observed from the flower extract of E. japonicum (132.08 ㎍㎖^-1) against HCT-116 cell line and the highest IC₅₀ value was observed from the root extract of P. communis (995.31 ㎍㎖^-1) against Hep3B cell line. However, the obtained results from our investigation is similar to the results obtained from previous investigation (Rahman et al.
5% respectively, and there was no distinct difference in the nitrite scavenging effects of flower and stem organs in the present study. In conclusion, the results obtained in the present study demonstrated the immune responses of the aerial parts of four resource plant related to its cytotoxic activities against cancer cell lines combined with nitrite scavenging activity. The results prevailed that the immune responses from both T and B cells were influenced by the time dependant manner and most of the studied resource plants have potential cytotoxic activity.
also showed similar tendency to the Hep3B cell line. It was found that the concentrations of 1,000 ㎍㎖^-1 or more of plant extracts was remarkably low the cell viability percentage. That is, at the highest concentration (2,000 ㎍㎖^-1) of all organs showed the highest toxicity against all cancer cell lines.
The highest scavenging ability (96.4%) was observed from the flower organ of Corylopsis coreana at a pH of 1.2 while the lowest scavenging activity (58.2%) was observed from the root extract of Phragmites communis. The result revealed that among all of the tested plants, Corylopsis coreana showed the highest scavenging activity followed by Erythronium japonica, Salocornia herbacea and Phagmites communis respectively.
2%) was observed from the root extract of Phragmites communis. The result revealed that among all of the tested plants, Corylopsis coreana showed the highest scavenging activity followed by Erythronium japonica, Salocornia herbacea and Phagmites communis respectively. Also it was prevailed that the organ, root showed more weak scavenging activity than that of other organs used in the present study.

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