Lee, Jong Hun
(Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University School of Medicine)
,
Han, Jin Woo
(Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University School of Medicine)
,
Byun, Jin Hwan
(Department of Plastic and Reconstructive Surgery, Eulji General Hospital, Eulji University School of Medicine)
,
Lee, Won Mi
(Department of Pathology, Eulji General Hospital, Eulji University School of Medicine)
,
Kim, Min Ho
(Eulji Medi-Bio Research Institute, Eulji University)
,
Wu, Wen Hao
(Eulji Medi-Bio Research Institute, Eulji University)
Background: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus myk...
Background: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus mykiss) and salmon (Oncorhynchus keta). The present study was designed to evaluate the wound healing effects of O. keta-derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O. mykiss-derived PDRN injection (Placentex). Methods: Full-thickness skin defects were made on the back of mice (n=60). The mice were divided into the following four groups according to the dressing used for the wounds: O. mykiss-derived PDRN injection group, O. keta-derived PDRN injection group, O. ketaderived PDRN cream group, and normal saline soaked dressing group (control group). We analyzed the gross findings, wound sizes, histological findings, immunohistochemistry and enzyme-linked immunosorbent assays for the groups immediately after the treatment, and again after 4, 7, and 10 days of treatment. Results: The wound healing effects were the greatest in the O. keta-derived PDRN injection and O. mykiss-derived PDRN injection groups, which showed similar scores, followed by the O. keta-derived cream and normal saline soaked dressing groups. Conclusion: The injection of PDRN extracted from O. keta was found to be as effective at healing full-thickness skin defects as the O. mykiss-derived PDRN injection, which is currently used in the clinic. Moreover, the O. keta-derived PDRN injection was also found to reduce the time required for wound healing.
Background: Polydeoxyribonucleotide (PDRN) influencing cellular growth and differentiation is recognized to promote wound healing by stimulating tissue repair. Although PDRN can be extracted from human placentas, PDRN medications have recently been extracted from the semen of trout (Oncorhynchus mykiss) and salmon (Oncorhynchus keta). The present study was designed to evaluate the wound healing effects of O. keta-derived PDRN for injection (Rejuvenex) and PDRN cream (Rejuvenex Cream) in comparison with those of O. mykiss-derived PDRN injection (Placentex). Methods: Full-thickness skin defects were made on the back of mice (n=60). The mice were divided into the following four groups according to the dressing used for the wounds: O. mykiss-derived PDRN injection group, O. keta-derived PDRN injection group, O. ketaderived PDRN cream group, and normal saline soaked dressing group (control group). We analyzed the gross findings, wound sizes, histological findings, immunohistochemistry and enzyme-linked immunosorbent assays for the groups immediately after the treatment, and again after 4, 7, and 10 days of treatment. Results: The wound healing effects were the greatest in the O. keta-derived PDRN injection and O. mykiss-derived PDRN injection groups, which showed similar scores, followed by the O. keta-derived cream and normal saline soaked dressing groups. Conclusion: The injection of PDRN extracted from O. keta was found to be as effective at healing full-thickness skin defects as the O. mykiss-derived PDRN injection, which is currently used in the clinic. Moreover, the O. keta-derived PDRN injection was also found to reduce the time required for wound healing.
4 Montesinos MC Gadangi P Longaker M Sung J Levine J Nilsen D Wound healing is accelerated by agonists of adenosine A2 (G alpha s-linked) receptors J Exp Med 1997 186 1615 20 9348321
5 Tonello G Daglio M Zaccarelli N Sottofattori E Mazzei M Balbi A Characterization and quantitation of the active polynucleotide fraction (PDRN) from human placenta, a tissue repair stimulating agent J Pharm Biomed Anal 1996 14 1555 60 8877863
6 Bitto A Polito F Altavilla D Minutoli L Migliorato A Squadrito F Polydeoxyribonucleotide (PDRN) restores blood flow in an experimental model of peripheral artery occlusive disease J Vasc Surg 2008 48 1292 300 18971038
7 Bianchini P Tellini N Morani AM Folloni MG Pharmacological data on polydeoxyribonucleotide of human placenta Int J Tissue React 1981 3 151 4 6460689
8 Guizzardi S Galli C Govoni P Boratto R Cattarini G Martini D Polydeoxyribonucleotide (PDRN) promotes human osteoblast proliferation: a new proposal for bone tissue repair Life Sci 2003 73 1973 83 12899922
9 Sini P Denti A Cattarini G Daglio M Tira ME Balduini C Effect of polydeoxyribonucleotides on human fibroblasts in primary culture Cell Biochem Funct 1999 17 107 14 10377956
10 Galeano M Bitto A Altavilla D Minutoli L Polito F Calo M Polydeoxyribonucleotide stimulates angiogenesis and wound healing in the genetically diabetic mouse Wound Repair Regen 2008 16 208 17 18318806
11 Bitto A Galeano M Squadrito F Minutoli L Polito F Dye JF Polydeoxyribonucleotide improves angiogenesis and wound healing in experimental thermal injury Crit Care Med 2008 36 1594 602 18434887
12 Thellung S Florio T Maragliano A Cattarini G Schettini G Polydeoxyribonucleotides enhance the proliferation of human skin fibroblasts: involvement of A2 purinergic receptor subtypes Life Sci 1999 64 1661 74 10328526
13 Belletti S Uggeri J Gatti R Govoni P Guizzardi S Polydeoxyribonucleotide promotes cyclobutane pyrimidine dimer repair in UVB-exposed dermal fibroblasts Photodermatol Photoimmunol Photomed 2007 23 242 9 17986061
14 Khoa ND Montesinos MC Reiss AB Delano D Awadallah N Cronstein BN Inflammatory cytokines regulate function and expression of adenosine A(2A) receptors in human monocytic THP-1 cells J Immunol 2001 167 4026 32 11564822
15 Chan ES Fernandez P Merchant AA Montesinos MC Trzaska S Desai A Adenosine A2A receptors in diffuse dermal fibrosis: pathogenic role in human dermal fibroblasts and in a murine model of scleroderma Arthritis Rheum 2006 54 2632 42 16871530
16 Nguyen DK Montesinos MC Williams AJ Kelly M Cronstein BN Th1 cytokines regulate adenosine receptors and their downstream signaling elements in human microvascular endothelial cells J Immunol 2003 171 3991 8 14530318
17 Yegutkin GG Nucleotide- and nucleoside-converting ectoenzymes: important modulators of purinergic signalling cascade Biochim Biophys Acta 2008 1783 673 94 18302942
18 Montesinos MC Desai A Chen JF Yee H Schwarzschild MA Fink JS Adenosine promotes wound healing and mediates angiogenesis in response to tissue injury via occupancy of A(2A) receptors Am J Pathol 2002 160 2009 18 12057906
20 Kim JY Pak CS Park JH Jeong JH Heo CY Effects of polydeoxyribonucleotide in the treatment of pressure ulcers J Korean Med Sci 2014 29 Suppl 3 S222 7 25473213
21 Polito F Bitto A Galeano M Irrera N Marini H Calo M Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps J Vasc Surg 2012 55 479 88 22051873
22 De Aloe G Rubegni P Biagioli M Taddeucci P Fimiani M Skin graft donor site and use of polydeoxyribonucleotide as a treatment for skin regeneration: a randomized, controlled, double-blind, clinical trial Wounds 2004 16 258 63
24 Ravenscroft MJ Harker J Buch KA A prospective, randomised, controlled trial comparing wound dressings used in hip and knee surgery: Aquacel and Tegaderm versus Cutiplast Ann R Coll Surg Engl 2006 88 18 22 16460632
※ AI-Helper는 부적절한 답변을 할 수 있습니다.