녹차뿌리 특화 사포닌의 천연 계면 활성력을 이용한 새로운 안티폴루션 기작 연구 Green Tea Root Is a Potential Natural Surfactant and Is Protective against the Detrimental Stimulant PM2.5 in Human Normal Epidermal Keratinocytes원문보기
녹차(Camellia sinensis L.)는 약용으로도 널리 사용되어 왔다. 하지만, 대부분의 연구는 녹차 잎을 연구의 대상으로 이루어졌으며, 지금까지도 잎 외의 다른 부분은 연구가 많이 이루어지지 않았다. 본 연구에서는 외부환경의 미세먼지(particulate matter 2.5; PM2.5)에 의한 normal human epidermal keratinocytes (NHEKs)의 손상을 뿌리 추출물이 효과적으로 보호할 수 있는 효과를 확인하였다. 30년 된 녹차뿌리 표본은 아모레퍼시픽의 Dolsongi tea field에서 채취되었으며 70%의 에탄올로 추출한 녹차 뿌리 추출물은 총 순수 사포닌 함량이 54%로, 인삼 추출물보다 더 많은 사포닌을 함유한 것을 확인하였다. 그리고 PM2.5에 의한 손상으로부터의 보호 효과를 평가하기 위해 건선환자의 특이적 바이오 마커인 IL-36G를 관찰한 결과 IL-36G mRNA 발현량은 PM2.5 처리시 녹차 뿌리 추출물 처리를 통해 IL-36G 식의 유의적인 감소를 확인할 수 있었다. 결론적으로, 30년 된 녹차뿌리 추출물은 사포닌 함량이 높은 자연 계면 활성제로 사용될 수 있으며, 또한 피부질환마커인 IL-36G 발현의 억제를 통해 PM2.5에 의해 유발되는 손상에 대한 보호 효과가 있음을 알 수 있었다.
녹차(Camellia sinensis L.)는 약용으로도 널리 사용되어 왔다. 하지만, 대부분의 연구는 녹차 잎을 연구의 대상으로 이루어졌으며, 지금까지도 잎 외의 다른 부분은 연구가 많이 이루어지지 않았다. 본 연구에서는 외부환경의 미세먼지(particulate matter 2.5; PM2.5)에 의한 normal human epidermal keratinocytes (NHEKs)의 손상을 뿌리 추출물이 효과적으로 보호할 수 있는 효과를 확인하였다. 30년 된 녹차뿌리 표본은 아모레퍼시픽의 Dolsongi tea field에서 채취되었으며 70%의 에탄올로 추출한 녹차 뿌리 추출물은 총 순수 사포닌 함량이 54%로, 인삼 추출물보다 더 많은 사포닌을 함유한 것을 확인하였다. 그리고 PM2.5에 의한 손상으로부터의 보호 효과를 평가하기 위해 건선환자의 특이적 바이오 마커인 IL-36G를 관찰한 결과 IL-36G mRNA 발현량은 PM2.5 처리시 녹차 뿌리 추출물 처리를 통해 IL-36G 식의 유의적인 감소를 확인할 수 있었다. 결론적으로, 30년 된 녹차뿌리 추출물은 사포닌 함량이 높은 자연 계면 활성제로 사용될 수 있으며, 또한 피부질환마커인 IL-36G 발현의 억제를 통해 PM2.5에 의해 유발되는 손상에 대한 보호 효과가 있음을 알 수 있었다.
Green tea (Camellia sinensis L.) has been widely explored for its medicinal applications. However, most of the studies had targeted the green tea leaf, while other parts remained unexplored. In this study, protective effect of green tea root extract on Normal Human Epidermal Keratinocytes (NHEKs) ag...
Green tea (Camellia sinensis L.) has been widely explored for its medicinal applications. However, most of the studies had targeted the green tea leaf, while other parts remained unexplored. In this study, protective effect of green tea root extract on Normal Human Epidermal Keratinocytes (NHEKs) against the damage induced by an external stimulant (PM2.5) was confirmed. Thirty-year-old green tea root samples were collected from Amorepacific's Dolsongi tea field and green tea root extract was prepared with 70% ethanol. Total crude saponin content in green tea root extract was 54%, which is much higher than that in ginseng extract. Our results suggest that green tea root extract can be used as a natural surfactant in cosmetics. For evaluating its protective effect against the damage induced by PM2.5, IL-36G was used as a biomarker. IL-36G mRNA expression level increased remarkable upon PM2.5 treatment in NHEKs. Moreover, IL-36G was recently reported to be expressed in psoriasis lesions. Results showed significant decrease of IL-36G expression by treatment of green tea root extract. In conclusion, thirty-year-old green tea root extract can be used as a natural surfactant with a high saponin content and may have protective effect against the damage induced by PM2.5.
Green tea (Camellia sinensis L.) has been widely explored for its medicinal applications. However, most of the studies had targeted the green tea leaf, while other parts remained unexplored. In this study, protective effect of green tea root extract on Normal Human Epidermal Keratinocytes (NHEKs) against the damage induced by an external stimulant (PM2.5) was confirmed. Thirty-year-old green tea root samples were collected from Amorepacific's Dolsongi tea field and green tea root extract was prepared with 70% ethanol. Total crude saponin content in green tea root extract was 54%, which is much higher than that in ginseng extract. Our results suggest that green tea root extract can be used as a natural surfactant in cosmetics. For evaluating its protective effect against the damage induced by PM2.5, IL-36G was used as a biomarker. IL-36G mRNA expression level increased remarkable upon PM2.5 treatment in NHEKs. Moreover, IL-36G was recently reported to be expressed in psoriasis lesions. Results showed significant decrease of IL-36G expression by treatment of green tea root extract. In conclusion, thirty-year-old green tea root extract can be used as a natural surfactant with a high saponin content and may have protective effect against the damage induced by PM2.5.
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문제 정의
This research is focused on evaluating the efficacies of roots of green tea trees on human skin and the analysis of its unique contents.
제안 방법
The reaction was allowed to proceed for 10 min at 55 ℃. Liquid chromatographic analysis was performed on a Waters Acquity UPLC system, equipped with a binary solvent manager, an autosampler, a column heater and a photodiode array (PDA) detector. The separation column was a Waters AccQ-TagTM Ultra column (2.
대상 데이터
30-year-old green tea root samples were collected in A more pacific Dosun tea garden (Jeju island, South Korea). Green tea root was extracted with 70% ethanol (v/v) at room temperature for 12 h with constant stir.
One microliter of sample was injected for analysis. The PDA detector was set at 260 nm, with a sampling rate of 20 points/sec.
이론/모형
In order to investigate the cytotoxicity of green tea root, Cell Counting Kit-8 (CCK-8) assay (Dojindo, MD, USA) was conducted. HaCat cells were seeded in triplicate in 96-well plate and green tea root extract was treated for 24 h on the next day.
The UPLC analysis of 21 kinds of amino acids and γ-aminobutyric acid was based on the Waters AccQ-TagTM method (http://www.waters.com).
Total crude saponin contents in green tea root extract were estimated by vanillin-sulfuric acid colorimetric methods[2]. To 100 µL of methanol solution of the sample, 0.
성능/효과
Green tea root extract did not affect NHEKs viability at below 50 ppm (Figure 2). In results, the mRNAs of inflammatory factor, IL-36G was induced by the external stimulant, PM2.5, but this was significantly decreased by green tea root extracts (Figure 3), and thus, improvement of psoriasis-related anti-inflammatory effects was observed. Green tea root extracts have a protective effect against the damage induced by the external stimulant (PM2.
It was recently reported that IL-36G is a biomarker in psoriasis[8]. In the report, IL-36G was expressed in only psoriasis lesions among inflammatory skin diseases(psoriasis, atopic dermatitis, lichen planus and contact eczema) markers. Therefore IL-36G is a therapeutic target for the detrimental effects of PM2.
참고문헌 (8)
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E. Uehara, H. Hokazono, M. Hida, T. Sasaki, H. Yoshioka, and N. Matsuo, GABA promotes elastin synthesis and elastin fiber formation in normal human dermal fibroblasts (HDFs), Biosci., Biotechnol., Biochem., 81, 1198-1205 (2017).
A. M. D'Erme, D. Wilsmann-Theis, J. Wagenpfeil, M. Holzel, S. Ferring-Schmitt, S. Sternberg, M. Wittmann, B. Peters, A. Bosio, T. Bieber, and J. Wenzel, IL- $36{\gamma}$ (IL-1F9) is a biomarker for psoriasis skin lesions, J. Invest. Dermatol., 135, 1025-1032 (2015).
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