[논문]피마자(Ricinus communis L.) 잎과 열매의 열수(熱水) 및 에탄올 추출물의 항균 활성과 항산화 효능

박장순

doi:10.14400/jdc.2018.16.6.353

[국내논문] 피마자(Ricinus communis L.) 잎과 열매의 열수(熱水) 및 에탄올 추출물의 항균 활성과 항산화 효능
Antimicrobial and Antioxidant Activity of Hot Water and Ethanol Extracts of Ricinus communis L. Leaves and Fruits 원문보기

디지털융복합연구 = Journal of digital convergence, v.16 no.6, 2018년, pp.353 - 361

초록
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천연 항균제 및 항산화제 개발의 일환으로 선택한 피마자 열매의 열수 추출물(RFW)과 에탄올 추출물(RFE)은 각각 15.8%와 18.4%의 수율을 나타냈다. Paper-disc method을 통한 항균활성 측정 결과, 피마자 열매를 에탄올로 추출한 추출물에서 항균활성이 나타났다. 특히 녹농균 및 황색포도상구균에서 활성이 우수하였으며, 캔디다균에서도 배양 16시간까지 1.5mm 정도의 항균활성이 나타났으나 24시간 이후에는 캔디다균의 증식이 다시 관찰되었다. 피마자 열매 에탄올추출물(RFE)의 MIC실험 결과 S. aureus와 P. aeruginosa에서 각각 96%와 93%의 항균력이 나타났다. 피마자 잎 열수추출물(RLW)과 피마자 잎 에탄올추출물(RLE)의 DPPH radical 소거능 측정을 통한 항산화 효능 결과는 $1000({\mu}g/m{\ell})$에서 각각 $1.8{\pm}0.6%$, $2.1{\pm}0.7%$의 free radical 소거율을 나타냈다. 본 연구가 향후 천연물을 이용한 항균제 및 항산화제 개발을 위한 기초 자료로 제공되리라 사료된다.

Abstract ▼ AI-Helper

RFW and RFE of castor fruit selected as part of the development of natural antimicrobials and antioxidants yielded 15.8% and 18.4% respectively. In the results of measuring the antimicrobial activity through paper disc method, the antimicrobial activity of castor fruits in ethanol extracts appeared. Especially, the activity was excellent in P. aeruginosa and S. aureus, and antimicrobial activity of C. was 1.5mm up to 16 hours. However, the proliferation of C. was observed again after 24 hours. In the MIC experiment results of RFE, S. aureus and P. aeruginosa showed 96% and 93% of antimicrobial activity, respectively. The DPPH radical scavenging activity of RLW and castor leaf ethanol extract showed $1.8{\pm}0.6%$ and $2.1{\pm}0.7%$ free radical scavenging activity at $1000({\mu}g/m{\ell})$. This study is expected to provide basic data for the development of antimicrobial agents and antioxidants using natural products.

Keyword

AI 본문요약
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문제 정의

coli and Pseudomonas aeruginosa, aerobic gram negative strains, and fungi Candia albicans and Aspergillus niger. By studying the antioxidant effect through DPPH radical scavenging activity, this study aims to provide basic data for the development of natural substances that can replace drugs composed of artificial compounds.

제안 방법

Hot water and RFE of castor leaves were prepared by putting 10g of leaf powder, 200 ml of sterilized distilled water and 200 ml of 80% ethanol into a 1 L Erlenmeyer flask, wrapped in foil, and extracted at 60℃ for 24 hours, and hot water and RFE of castor fruits were prepared by putting 7g of powdered beans, 140ml of sterilized distilled water and 140ml of 80% ethanol in a 1 L Erlenmeyer flask, wrapped in foil, and extracted at 60℃ for 24 hours.
The antimicrobial activity of castor fruits RFE (hereinafter referred to as RFE) was measured by modifying the minimum inhibitory concentration (MIC) assay. First, sterilize the TSA medium and cool to 45～50℃ after sterilization.

대상 데이터

The strains used in the experiment were three strains consisting of one Gram (+) strain and two Gram (-) strains. The aerobic Gram (+) was S.
aeruginosa (KCTC1637). C. albicans (KCTC7965) causing candidiasis was the fungus, and A. niger (KCTC6910), known as black germ, was purchased from the BRC (KCTC) and used.
Castor leaves distilled water extract (hereinafter referred to as RLW) and castor leaves RFE (hereinafter referred to as RLE) used in this antioxidant experiment were supplied by Soongsil University, and was used after the selection in TBRC RIC of Daejeon University.

이론/모형

The antimicrobial activity experiment of castor leaf and bean extracts was conducted to measure the prepared four samples with the paper disc method. First, a single colony of each strain with good activity is collected through subculture, and after uniformly streaking over the entire solid medium, the sterilized paper disc is placed on the smear plate and 50㎕ of each extract is collected.

성능/효과

The RFW (Ricinus communis L. Extract ; RFW) and RFE(Ricinus communis L. Extract; RFE) of castor fruits were 1.104g and 1.29g, respectively at 7g, and so the extraction yields were 15.8% and 18.4%, respectively.
From the results of minimum inhibitory concentration of castor fruits RFE, E. coli, C. albicans, and A. niger did not exhibit any significant antimicrobial activity while S. aureus and P. aeruginosa showed outstanding 96% and 93% of antimicrobial activity, respectively.

후속연구

Therefore, even if a specific ingredient is developed with the entire part of a castor bean, the issues of ricin non detection test results, basic cytotoxicity, and other toxicity of castor fruits may still arise. Therefore, further studies should clarify the safety and progress of ricin contained in castor beans.

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