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Anti-inflammatory Effects of Abeliophyllum distichum Flower Extract and Associated MAPKs and NF-κB Pathway in Raw264.7 Cells 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.31 no.3, 2018년, pp.202 - 210  

Lee, Jin-Wook (Department of Biomedical Science, Jungwon University) ,  Kang, Yoon-Joong (Department of Biomedical Science, Jungwon University)

Abstract AI-Helper 아이콘AI-Helper

Abeliophyllum distichum is a medicinal plant used in regional traditional medicine to relieve pain in inflammatory processes. In this study, anti-inflammatory effects of Abeliophyllum distichum flower (ADF) extract were examined. Furthermore, possible molecular mechanisms of the anti-inflammatory ef...

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제안 방법

  • MMP-9 expression is regulated through the activity of NF-κB, AP-1 transcription factor and pro-inflammatory cytokine. To investigate effects of ADF on LPS-stimulated MMP-9 expressing Raw264.7 cells, we carried out Gelatin Zymography. Raw264.

대상 데이터

  • Abeliophyllum distichum (voucher number: Park 1001(ANH)) were collected and identified by Jae Ho Park (Jungwon University, Goesan, Korea) at Goesan-gun, Chungbuk, Korea. Five hundred grams of flowers were extracted with 1,000 ㎖ of 80% methanol.

데이터처리

  • For comparisons, analysis was carried out using one-way analysis of variance (ANOVA) followed by using GraphPad Prism 5.0 program (GraphPad software, CA, USA). P < 0.

이론/모형

  • Nitrite Oxide concentration in the medium was measured using the Griess reaction method. In brief, cells were seeded at 1×106 cells/well in 24-well plates (SPL, Gyeonggi, Korea).
  • The cell suspensions were centrifuged at 13,000 rpm at 4℃ for 5 min. Protein concentrations were confirmed using Bradford protein assay. The protein separated in 10% SDS-PAGE gel and transferred to polyvinylidenedifluoride (PVDF) membrane (Millipore, MA, USA).
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