레드비트를 함유하는 화장품의 담배 연기에 의한 피부 지질 산화 방지 효과 Protective Effect of Cosmetics Containing Red Beet against Cigarette Smoke-induced Oxidative Damage in Human Skin원문보기
최근 화장품 시장에서 안티폴루션 효능이 있는 화장품은 새로운 피부 건강을 위한 해결책으로 나타나고 있다. 외부(대기) 오염물질에 의해 피부의 산화 기전을 매개하는 주요 원인 인자들로 오존, UV, 미세먼지 및 담배연기 등을 꼽을 수 있다. 담배 연기의 노출은 직 간접적으로 피부 표피 내 지질의 산화를 야기한다. 피부의 지질 산화에 의해 squalene과 squalene monohydroperoxide의 비율 변화가 발생하고, malondialdehyde (MDA)가 지질산화 산물로 생성된다. 따라서, 본 연구에서는 담배연기 노출 시 발생하는 피부 산화에 대하여 레드비트를 함유하는 화장품이 방지할 수 있는 효능이 있는지에 대하여 MDA의 생성량으로 관찰하였다. 시험대상자 전박부를 세 영역(음성대조, 양성대조, 시험제품)으로 나누어 각 지름 3.3 cm의 원으로 구획하고, 15분간 담배연기에 노출 후 피부 표면으로부터 지질막을 걷어낸 후 TBARS assay를 통하여 MDA를 정량 하였다. 음성대조(무도포, 미노출)에 비해 양성대조(무도포, 담배연기 노출)의 MDA 생성량이 3.7배 증가된 결과로, 오염물질인 담배연기에 의한 피부의 산화적 손상을 확인하였다. 반면에 레드비트를 함유하는 화장품을 미리 도포한 영역은 양성대조에 비해 MDA 생성량이 25% 감소하는 결과를 나타내었다. 결론적으로, 담배연기 노출은 피부표피 내 지질 산화를 야기하며, 레드비트를 함유하는 화장품이 이러한 대기환경오염으로부터 방어적 효과(안티폴루션 효과)를 보이는 것을 확인하였다.
최근 화장품 시장에서 안티폴루션 효능이 있는 화장품은 새로운 피부 건강을 위한 해결책으로 나타나고 있다. 외부(대기) 오염물질에 의해 피부의 산화 기전을 매개하는 주요 원인 인자들로 오존, UV, 미세먼지 및 담배연기 등을 꼽을 수 있다. 담배 연기의 노출은 직 간접적으로 피부 표피 내 지질의 산화를 야기한다. 피부의 지질 산화에 의해 squalene과 squalene monohydroperoxide의 비율 변화가 발생하고, malondialdehyde (MDA)가 지질산화 산물로 생성된다. 따라서, 본 연구에서는 담배연기 노출 시 발생하는 피부 산화에 대하여 레드비트를 함유하는 화장품이 방지할 수 있는 효능이 있는지에 대하여 MDA의 생성량으로 관찰하였다. 시험대상자 전박부를 세 영역(음성대조, 양성대조, 시험제품)으로 나누어 각 지름 3.3 cm의 원으로 구획하고, 15분간 담배연기에 노출 후 피부 표면으로부터 지질막을 걷어낸 후 TBARS assay를 통하여 MDA를 정량 하였다. 음성대조(무도포, 미노출)에 비해 양성대조(무도포, 담배연기 노출)의 MDA 생성량이 3.7배 증가된 결과로, 오염물질인 담배연기에 의한 피부의 산화적 손상을 확인하였다. 반면에 레드비트를 함유하는 화장품을 미리 도포한 영역은 양성대조에 비해 MDA 생성량이 25% 감소하는 결과를 나타내었다. 결론적으로, 담배연기 노출은 피부표피 내 지질 산화를 야기하며, 레드비트를 함유하는 화장품이 이러한 대기환경오염으로부터 방어적 효과(안티폴루션 효과)를 보이는 것을 확인하였다.
In cosmetics market, anti-pollution products recently come up with new solution for skin health. Environmental oxidation mechanisms are realized as bio-marker of atmospheric pollution upon skin by environmental pollutant such as ozone, UV rays, particulate matter (PM) as well as cigarette smoke. The...
In cosmetics market, anti-pollution products recently come up with new solution for skin health. Environmental oxidation mechanisms are realized as bio-marker of atmospheric pollution upon skin by environmental pollutant such as ozone, UV rays, particulate matter (PM) as well as cigarette smoke. The exposure of cigarette smoke directly or indirectly causes the oxidation of the stratum corneum skin lipids, resulting in the conversion of squalene to squalene monohydroperoxide and/or generation of malondialdehyde (MDA) as a product of lipid peroxidation. The aim of this study is to see whether new cosmetics product containing red beet has anti-oxidation effect on skin exposed by cigarette smoke. So as to determine oxidative damage to human skin at biochemical level, each unit area of volar forearms was exposed to cigarette smoke through device (3.3 cm, diameter) for fifteen minutes, then measured MDA using standardized TBARS assay kit. Compared to negative control (untreated and unexposed area), the level of MDA was significantly increased at positive control (untreated and exposed area) more than 3.7 times, indicating the pollutant induced-oxidative damage on the skin barrier. Whereas, the pre-applied area with the cosmetics products containing red beet revealed a decrease of 25% compared with positive control. As reports, these data demonstrated that cigarette smoke induce peroxidation of stratum corneum skin lipids. Conclusively, we suggest that anti-pollution effect of the cosmetics product containing red beet is beneficial to prevent the oxidation of skin lipids by atmospheric pollution.
In cosmetics market, anti-pollution products recently come up with new solution for skin health. Environmental oxidation mechanisms are realized as bio-marker of atmospheric pollution upon skin by environmental pollutant such as ozone, UV rays, particulate matter (PM) as well as cigarette smoke. The exposure of cigarette smoke directly or indirectly causes the oxidation of the stratum corneum skin lipids, resulting in the conversion of squalene to squalene monohydroperoxide and/or generation of malondialdehyde (MDA) as a product of lipid peroxidation. The aim of this study is to see whether new cosmetics product containing red beet has anti-oxidation effect on skin exposed by cigarette smoke. So as to determine oxidative damage to human skin at biochemical level, each unit area of volar forearms was exposed to cigarette smoke through device (3.3 cm, diameter) for fifteen minutes, then measured MDA using standardized TBARS assay kit. Compared to negative control (untreated and unexposed area), the level of MDA was significantly increased at positive control (untreated and exposed area) more than 3.7 times, indicating the pollutant induced-oxidative damage on the skin barrier. Whereas, the pre-applied area with the cosmetics products containing red beet revealed a decrease of 25% compared with positive control. As reports, these data demonstrated that cigarette smoke induce peroxidation of stratum corneum skin lipids. Conclusively, we suggest that anti-pollution effect of the cosmetics product containing red beet is beneficial to prevent the oxidation of skin lipids by atmospheric pollution.
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문제 정의
Red beet root was evident at lowering the level of microsomal lipid peroxidation in the liver and enhancing the activity of super-oxide dismutase[9]. As these evidences, we performed in this study whether a product containing red beet would be reduce the lipid peroxidation induced by cigarette smoke on human skin.
제안 방법
As previous reported[8], the exposure of cigarette smoke caused lipid oxidation on human skin in our experiment. In this study, the red beet was potent active ingredient for anti-oxidative activation, showing DPPH and superoxide radicals scavenging in vitro. The effectiveness on anti-oxidation of the red beet was repeatable in human, lowering MDA production.
The volar forearms of 13 subjects was exposed to cigarette smoke for 15 min after product application or nothing applied. The collected corneum lipids by swabbing were analyzed for MDA production, a lipid peroxidation marker, using TBARS assay. The level of MDA was significantly increased approximately 3.
대상 데이터
Cigarette smoke was applied simultaneously on each site using the air compressor chamber. Six cigarettes were used for one application.
This study was performed with subjects who were aged 20 to 60. Subjects were total of 13 people, 12 males and 1 female, and the average age was 40.
데이터처리
0 was used to evaluate the efficacy of test product for skin changes. The parametric-test, Independent t-test was used according to the statistical treatment for analysis.
참고문헌 (11)
P. Schroeder, S. M. Schieke, and A. Morita, Premature skin aging by infrared radiation, tobacco smoke and ozone, Skin Aging, 45 (2006).
K. Donaldson, L. Tran, L. A. Jimenez, R. Duffin, D. E. Newby, N. Mills, W. MacNee, and V. Stone, Combustion-derived nanoparticles: a review of their toxicology following inhalation exposure, Part Fibre Toxicol., 21, 2 (2005).
C. Lahmann, J. Bergemann, G. Harrison, and A. R. Young, Matrix metalloproteinase-1 and skin ageing in smokers, Lancet., 357, 935 (2001).
F. Yildirim Bas, D. Bayram, B. Arslan, I. Armagan, S. Yesilot, E. Yesilot, and E. Yorgancigil, Effect of alpha lipoic acid on smoking-induced skin damage, Cutan Ocul Toxicol., 36, 67 (2017).
L. Yin, A. Morita and, T. Tsuji, Skin aging induced by ultraviolet exposure and tobacco smoking: evidence from epidemiological and molecular studies, Photodermatol. Photoimmunol. Photomed., 17, 178 (2001).
M. Kujawska, E. Ignatowicz, M. Murias, M. Ewertowska, K. Mikolajczyk, and J. Jodynis-Liebert, Protective effect of red beetroot against carbon tetrachloride- and N-nitrosodiethylamine-induced oxidative stress in rats, J. Agric. Food Chem., 25, 2570 (2009).
M. S. Blois, Antioxidant determinations by the use of a stable free radical, Nature, 29, 1199 (1958).
I. Parejo, F. Viladomat, J. Bastida, A. Rosas-Romero, N. Flerlage, J. Burillo, and C. Codina, Comparison between the radical scavenging activity and antioxidant activity of six distilled and nondistilled mediterranean herbs and aromatic plants, J. Agric. Food Chem., 50, 6882 (2002).
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