함초의 보충식이가 고지방식이 흰쥐의 혈청 및 조직의 중성지방 농도와 골격근 내 PGC-1α 및 PPAR-γ 단백질 발현에 미치는 영향 Effect of Salicornia herbacea L. Supplementation on Tissue Triglyceride Concentrations and PGC-1α & PPAR-γ Expression of Skeletal Muscle of Rats Fed a High-fat Diet원문보기
본 연구는 함초의 보충식이가 고지방식이 흰쥐의 혈청 및 조직의 중성지방 농도와 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현에 미치는 영향에 대하여 연구하였다. SD계 수컷 흰쥐를 대조군(CD, n=8), 고지방식이군(HD, n=8), 고지방식이+ 5% 함초 보충식이군(SD, n=8)으로 분류하여 8주간 사육하였다. 그 결과 SD군의 지방조직 중량은 HD군에 비해 약 25%정도 유의하게 감소한 반면 골격근의 중량은 SD군이 HD군에 비해 약 5%정도 유의하게 증가하였다(p<0.01). SD군의 혈청과 간장 내 중성지방은 HD군에 비해 약 20% 유의하게 감소하였다(p<0.05). SD군의 골격근 내 $PGC-1{\alpha}$ 와 $PPAR-{\gamma}$ 단백질 발현은 HD군에 비해 1.5배 유의하게 높게 나타났다(p<0.01). 이상의 결과로 부터 함초 보충은 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현의 증가를 통하여 지방감소 및 근육량 증가에 효과적이었음이 시사되었다.
본 연구는 함초의 보충식이가 고지방식이 흰쥐의 혈청 및 조직의 중성지방 농도와 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현에 미치는 영향에 대하여 연구하였다. SD계 수컷 흰쥐를 대조군(CD, n=8), 고지방식이군(HD, n=8), 고지방식이+ 5% 함초 보충식이군(SD, n=8)으로 분류하여 8주간 사육하였다. 그 결과 SD군의 지방조직 중량은 HD군에 비해 약 25%정도 유의하게 감소한 반면 골격근의 중량은 SD군이 HD군에 비해 약 5%정도 유의하게 증가하였다(p<0.01). SD군의 혈청과 간장 내 중성지방은 HD군에 비해 약 20% 유의하게 감소하였다(p<0.05). SD군의 골격근 내 $PGC-1{\alpha}$ 와 $PPAR-{\gamma}$ 단백질 발현은 HD군에 비해 1.5배 유의하게 높게 나타났다(p<0.01). 이상의 결과로 부터 함초 보충은 골격근 내 $PGC-1{\alpha}$와 $PPAR-{\gamma}$ 단백질 발현의 증가를 통하여 지방감소 및 근육량 증가에 효과적이었음이 시사되었다.
This study examined whether the supplementation of Salicornia herbacea L. (SH), a member of the Chenopodiaceae subfamily, affects tissue specific triglyceride (TG) accumulation and the peroxisome proliferator-activated $receptor-{\gamma}$$coactivator-1{\alpha}$ ($PGC-1{\a...
This study examined whether the supplementation of Salicornia herbacea L. (SH), a member of the Chenopodiaceae subfamily, affects tissue specific triglyceride (TG) accumulation and the peroxisome proliferator-activated $receptor-{\gamma}$$coactivator-1{\alpha}$ ($PGC-1{\alpha}$) and peroxisome proliferator-activated $receptor-{\gamma}$ ($PPAR-{\gamma}$) protein expressions of skeletal muscle in rats with a high-fat diet. Sprague-Dawley male rats were randomly divided into three groups: control normal diet group (CD), high-fat diet group (HD), and 5.0% SH supplemented high-fat diet group (SD). The weights of fat tissue of the SD group were reduced by approximately 25%(p<0.01), while the skeletal muscle weight of the SD group increased approximately 5% compared to those in the HD group (p<0.01). The serum and hepatic TG of the SD group decreased approximately 20% compared to those of the HD group (p<0.05). In the protein expression levels in the skeletal muscle, the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ expressions of the SD group were 1.5-folds higher than those of the HD group (p<0.01). From these results, SH supplementation contributes to the improvement of the serum and hepatic TG concentrations, and the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ protein expression levels in the skeletal muscle of fed a high-fat diet. Thus, SH supplementation was effective in reducing fat mass and increasing muscle mass.
This study examined whether the supplementation of Salicornia herbacea L. (SH), a member of the Chenopodiaceae subfamily, affects tissue specific triglyceride (TG) accumulation and the peroxisome proliferator-activated $receptor-{\gamma}$$coactivator-1{\alpha}$ ($PGC-1{\alpha}$) and peroxisome proliferator-activated $receptor-{\gamma}$ ($PPAR-{\gamma}$) protein expressions of skeletal muscle in rats with a high-fat diet. Sprague-Dawley male rats were randomly divided into three groups: control normal diet group (CD), high-fat diet group (HD), and 5.0% SH supplemented high-fat diet group (SD). The weights of fat tissue of the SD group were reduced by approximately 25%(p<0.01), while the skeletal muscle weight of the SD group increased approximately 5% compared to those in the HD group (p<0.01). The serum and hepatic TG of the SD group decreased approximately 20% compared to those of the HD group (p<0.05). In the protein expression levels in the skeletal muscle, the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ expressions of the SD group were 1.5-folds higher than those of the HD group (p<0.01). From these results, SH supplementation contributes to the improvement of the serum and hepatic TG concentrations, and the $PGC-1{\alpha}$ and $PPAR-{\gamma}$ protein expression levels in the skeletal muscle of fed a high-fat diet. Thus, SH supplementation was effective in reducing fat mass and increasing muscle mass.
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가설 설정
It is well known that the lifestyle habits of humans in modern society, such as a high-fat diet and physical inactivity, promote weight gain, dyslipidemia, and profound systemic perturbations in lipid metabolism. In response to the small number of investigations carried out thus far to evaluate the effect of SH on lipid accumulation, in this study, we examined whether SH supplementation affects tissue-specific lipid metabolism characteristics such as weight and TG accumulation in rats with a high-fat diet.
제안 방법
Considering the above, the present investigation was carried out to evaluate the effect of SH supplementation on changes in weight, lipid accumulation according to specific tissue, and changes in protein expression regulating lipid metabolism such as PGC-1α and PPAR-γ in rats fed a highfat diet.
대상 데이터
As shown in Table 1, the HD consisted of 20% protein, 48% carbohydrate, and 20% fat, as modified from a previous study [6] and based on AIN-76G. Dried SH powder was purchased from Buan Hamcho (Buan, Cheonranam-do, S. Korea). The SD was prepared by using 5% SH and substituting a portion of the fiber component with the HD.
Six-week- old male Sprague-Dawley rats were obtained (Samtako Co., Osan, Korea) and individually housed in a controlled environment at 23±1℃ at 50±5% relative humidity, under a 12 hr light-dark cycle.
데이터처리
, USA). All statistical analyses were conducted using one-way ANOVA followed by the Least Significant Difference (LSD) post hoc test. Statistical significance was set at p<0.
성능/효과
1. During the obesity-inducing period, regardless of SH supplementation, the body weights of the high-fat diet groups such as SD and HD groups significantly increased compared to those in the CD group (p<0.01).
2. The weights of the fat pad and liver in the HD group were significantly higher than those in the CD group (p<0.01), while the fat and liver weights in the SD group significantly reduced compared to those in the HD group (p<0.01, p<0.05).
After the obesity-inducing period, the body weight of the SD group was significantly lower than that of the HD group, whereas the body weight of the HD group was significantly higher than that of the CD group (p<0.05).
Interestingly, while muscle hypertrophy was shown in the SD group, muscle TG did not show significant improvement in the SD group compared to the HD group. From these results, it could be assumed that the TG utilization within muscles was limited by only SH supplementation. Thus, further studies, considering the muscle TG metabolism and catabolism, are needed to better elucidate the relationship between muscle weight and TG concentration.
In conclusion, SH supplementation may play a role in enhancing muscle hypertrophy and improving the weights of fat tissue and liver in rats fed a high-fat diet. In addition, Salicornia herbacea L.
후속연구
From these results, it could be assumed that the TG utilization within muscles was limited by only SH supplementation. Thus, further studies, considering the muscle TG metabolism and catabolism, are needed to better elucidate the relationship between muscle weight and TG concentration.
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