[논문]Analysis of the Inhibitory Effect of two Bacterial Strains on Metarhizium anisopliae Induced Fatality Rates in Protaetia Brevitarsis

Kwak, Kyu-Won; Nam, Sung-Hee; Park, Kwan-Ho; Lee, Heuisam; Han, Myung-Sae

doi:10.7852/ijie.2018.37.1.9

Analysis of the Inhibitory Effect of two Bacterial Strains on Metarhizium anisopliae Induced Fatality Rates in Protaetia Brevitarsis 원문보기

International journal of industrial entomology, v.37 no.1, 2018년, pp.9 - 14

Kwak, Kyu-Won (National Institute of Agricultural Science, Department of Agricultural Biology) , Nam, Sung-Hee (National Institute of Agricultural Science, Department of Agricultural Biology) , Park, Kwan-Ho (National Institute of Agricultural Science, Department of Agricultural Biology) , Lee, Heuisam (National Institute of Agricultural Science, Department of Agricultural Biology) , Han, Myung-Sae (Kyungpook National University, Department of Bio-fibers and Materials Science)

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Bacterial species, Bacillus amyloliquefaciens and Lactobacillus species (L. sp.5-1), are known to inhibit the growth of pathogenic bacteria and fungi. Metarhizium anisopliae is a pathogenic fungal species which causes fatal damage to P. brevitarsis populations. Therefore, we investigated the inhibitory effect of B. amyloliquefaciens and L. sp. 5-1 on M. anisopliae induced fatality rates in P. brevitarsis. Samples of M. anisopliae-infected sawdust were treated with strain B. amyloliquefaciens KACC10116, strain L. sp. 5-1 KACC19351, and a combination of the two. P. brevitarsis were fed treated sawdust samples, and their subsequent fatality rate was monitored. The fatality rate fell below 1.5% after 10 days and decreased by approximately 40% after 15 days. On average, the fatality rate decreased by 20%, compared to the control. The difference in the decrease in fatality rate between B. amyloliquefaciens treatment and L. sp. 5-1 treatment was not significant. Results indicate that both strains exhibit high anti-fungal activity, which may be useful in environmental purification efforts. These strains may be used for effective prevention of fungal infection in P. brevitarsis.

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표/그림 (3)

그림 Fig. 1. P. brevitarsis mortality rate between B. amyloliquefaciens treatment and control groups in M. anisopliae sawdust. The mortality rate of control groups on third instar P. brevitarsis was about 68% after 15 days, but the mortality rate of the B.a. (OD₆₀₀= 1.5) treatment group was about 46%.
그림 Fig. 2. P. brevitarsis mortality rate between L. sp.5-1 treatment and control groups in M. anisopliae sawdust. The mortality rate of control groups on third instar P. brevitarsis was about 68% after 15 days, but the mortality rate of the L. sp. 5-1 (OD₆₀₀=2) treatment group was about 40%.
그림 Fig. 3. P. brevitarsis mortality rate between B. amyloliquefaciens plus L. sp.5-1 treatment and control groups in M. anisopliae sawdust. The mortality rate of control groups on third instar P. brevitarsis was about 68% after 15 days, but the mortality rate of the B.a (OD₆₀₀=1.0) plus L. sp.5-1(OD₆₀₀=1) treatment group was about 40%.

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sp. 5-1 KACC19351 were obtained from the Korean Agricultural Culture Collection, Rural Development Administration, Wanju, Korea. The Bacillus strain was grown on Nutrient Agar (NA; BD, Franklin Lakes, NJ, USA), Reasoner’s 2A agar (R2A; BD, Franklin Lakes, NJ, USA), and the Lactobacillus strain on de Man Rogosa and Sharpe (MRS; BD, Franklin Lakes, NJ, USA), respectively, for 8 hours in an incubator at 30°C.

참고문헌 (23)

Ahmed ST, Islam MM, Mun H, Sim H, Kim Y, Yang C (2014) Effects of Bacillus amyloliquefaciens as a probiotic strain on growth performance, cecal microflora, and fecal noxious gas emissions of broiler chickens. Poult Sci 93, 1963-1971.

상세보기
Arnison PG, Bibb MJ, Bierbaum G, Bowers AA, Bugni TS, Bulaj G, et al. (2013) Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat Prod Rep 30(1), 108-160.

상세보기
Beloeil PA, Chauvin C, Toquin MT, Fablet C, Le NoA tre Y, Salvat G, et al. (2003) Listeria monocytogenes contamination of finishing pigs: an exploratory epidemiological survey in France. Vet Res 34, 737-748.

상세보기
Benitez LB, Velho RV, Lisboa MP, Medina LF, Brandelli A (2010) Isolation and Characterization of Antifungal Peptides Produced by Bacillus amyloliquefaciens LBM5006. Int J Microbiol 48(6), 791-7.
Borgiat PT, Campbell LL (1978) ${\alpha}$ -Amylase from five strains of Bacillus amyloliquefaciens:evidence for identical primary structures. J Bacteriol 134, 389-393.
Brul S, Coote P (1999) Preservative agents in foods. Mode of action and microbial resistance mechanisms. Int J Food Microbiol 50, 1-17.

상세보기
Guimaraes A, Santiago A, Teixeira JA, Venancio A, Abrunhosa L (2018) Anti-aflatoxigenic effect of organic acids produced by Lactobacillus plantarum. Int J Microbiol, 284, 31-38.

상세보기
Huang R, Tao X, Wan C, Li S, Xu H, Xu F, et al. (2014) In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiots of mice. J Diary Sci 98, 5850-5861.
Idriss EE, Makarewicz O, Farouk A, Rosner K, Greiner R, Bochow H, et al. (2002) Extracellular phytase activity of Bacillus amyloliquefaciens FZB45 contributes to its plant-growth-promoting effect. Microbiol 148, 2097-2109.

상세보기
Koumoutsi A, Chen XH, Henne A, Liesegang H, Hitzeroth G, Franke P, et al. (2004) Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42. J Bacteriol 186, 1084-1096.

상세보기
Kwak KW, Kwon SW, Nam SH, Park KH, Kim ES, Lee HS, et al. (2018) Inhibition of Metarhizium anisopliae infection of Protaetia brevitarsis seluensis larvae using several effective microorganisms. Int J Indust Entomol 36, 1-9.
Kwak KW, Han MS, Nam SH, Park KH, Kim ES, Lee S, et al. (2016) Effect of Saccharomyces cerevisiae consumption on the pathogenicity of Beauveria bassiana in Protaetia brevitarsis. Int J Indust Entomol 33, 1-5.

원문보기 상세보기
Laitila A, Alakomi HL, Raaska L, Mattila-Sandholm T, Haikara A (2002) Antifungal activities of two Lactobacillus plantarum strains against Fusarium moulds in vitro and in malting of barley. J Appl Microbiol 93, 566-576.

상세보기
Lee A, Cheng KC, Liu JR (2017) Isolation and characterization of a Bacillus amyloliquefaciens strain with zearalenone removal ability and its probiotic potential. PloS One 12(8), 1-21.
Lee GH, Ryu CM (2016) Spraying of Leaf-colonizing Bacillus amyloliquefaciens protects pepper from cucumber mosaic virus. Plant Disease 100, 2099-2105.

상세보기
Li X, Quan CS, Yu HY, Wang JH, Fan S (2009) Assessment of antifungal effects of a novel compound from Burkholderia cepacia against Fusarium solani by fluorescent staining. World J Microbiol Biotechnol 25, 151-154.

상세보기
Liu J, Zhou T, He D, Li XZ, Wu H, Liu W, et al. (2011) Functions of lipopeptides bacillomycin D and fengycin in antagonism of Bacillus amyloliquefaciens C06 towards Monilinia fructicola. J Mol Microbiol Biotechnol 20, 43-52.

상세보기
Logan NA (2012) Bacillus and relatives in foodborne illness. J Appl Microbiol 112, 417-429.

상세보기
Meena KR, Kanwar SS (2015) Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics. BioMed Res Intl 2015, 473050.
Ongena M, Jacques P (2008) Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends Microbiol 16(3), 115-125.

상세보기
Poppi LB, Rivaldi JD, Coutinho TS, Astolfi-Ferreira CS, Ferreira AJP, Mancilha IM (2015) Effect of Lactobacillus sp. isolates supernatant on Escherichia coli O157:H7 enhances the role of organic acids production as a factor for pathogen control. Pesquisa Veterinaria Brasileira 35, 353-359.

상세보기
Russo P, Fares C, Longo A, Spano G, Capozzi V (2017) Lactobacillus plantarum with Broad Antifungal Activity as a Protective Starter Culture for Bread Production, Foods 176, 1-9.
Yu GY, Sinclair JB, Hartman GL, Bertagnolli BL (2002) Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biol Biochem 34, 955-963

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