Effects of fermented black ginseng on wound healing mediated by angiogenesis through the mitogen-activated protein kinase pathway in human umbilical vein endothelial cells원문보기논문타임라인
Background: Fermented black ginseng (FBG) is produced through several cycles of steam treatment of raw ginseng, at which point its color turns black. During this process, the original ginsenoside components of raw ginseng (e.g., Re, Rg1, Rb1, Rc, and Rb2) are altered, and less-polar ginsenosides are...
Background: Fermented black ginseng (FBG) is produced through several cycles of steam treatment of raw ginseng, at which point its color turns black. During this process, the original ginsenoside components of raw ginseng (e.g., Re, Rg1, Rb1, Rc, and Rb2) are altered, and less-polar ginsenosides are generated (e.g., Rg3, Rg5, Rk1, and Rh4). The aim of this study was to determine the effect of FBG on wound healing. Methods: The effects of FBG on tube formation and on scratch wound healing were measured using human umbilical vein endothelial cells (HUVECs) and HaCaT cells, respectively. Protein phosphorylation of mitogen-activated protein kinase was evaluated via Western blotting. Finally, the wound-healing effects of FBG were assessed using an experimental cutaneous wounds model in mice. Results and Conclusion: The results showed that FBG enhanced the tube formation in HUVECs and migration in HaCaT cells. Western blot analysis revealed that FBG stimulated the phosphorylation of p38 and extracellular signal-regulated kinase in HaCaT cells. Moreover, mice treated with $25{\mu}g/mL$ of FBG exhibited faster wound closure than the control mice did in the experimental cutaneous wounds model in mice.
Background: Fermented black ginseng (FBG) is produced through several cycles of steam treatment of raw ginseng, at which point its color turns black. During this process, the original ginsenoside components of raw ginseng (e.g., Re, Rg1, Rb1, Rc, and Rb2) are altered, and less-polar ginsenosides are generated (e.g., Rg3, Rg5, Rk1, and Rh4). The aim of this study was to determine the effect of FBG on wound healing. Methods: The effects of FBG on tube formation and on scratch wound healing were measured using human umbilical vein endothelial cells (HUVECs) and HaCaT cells, respectively. Protein phosphorylation of mitogen-activated protein kinase was evaluated via Western blotting. Finally, the wound-healing effects of FBG were assessed using an experimental cutaneous wounds model in mice. Results and Conclusion: The results showed that FBG enhanced the tube formation in HUVECs and migration in HaCaT cells. Western blot analysis revealed that FBG stimulated the phosphorylation of p38 and extracellular signal-regulated kinase in HaCaT cells. Moreover, mice treated with $25{\mu}g/mL$ of FBG exhibited faster wound closure than the control mice did in the experimental cutaneous wounds model in mice.
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문제 정의
In this study, we aim to assess the influence of FBG, which includes various bioactive ingredients, on wound healing by in vitro and in vivo experiments and to verify the possibility of using FBG as a wound healing medical ingredient.
제안 방법
The effect of FBG on the scratch wound healing in HaCaT cells was tested using a cell-based wound healing model in which scratch wounds were formed in the HaCaT cell monolayer (Fig. 4A). The repaired percentage of scarification in the presence of 25 μg/mL of FBG was 45.
Proteins (30 μg/lane) were separated by electrophoresis, transferred onto polyvinylidene fluoride membranes, and allowed to bind with epitope-specific primary and secondary antibodies. Visualization of antibody bounding was confirmed using ECL Advance Western Blotting Detection Reagents (GE Healthcare, Buckinghamshire, UK) and a LAS 4000 imaging system (Fujifilm, Tokyo, Japan) following instruction manual.
대상 데이터
Animal testing regulations were approved by the animal research and ethics committee of Gachon University. Male ICR mice (age, 5 wk) were purchased from Orient Bio Co., Ltd. (Seongnam, Korea) and were allocated into three experimental groups. Under light anesthesia with ethyl ether, a 5-mm full-width excisional skin wound was made in the shaved back of each mouse.
The mobile phase consisted of 15% acetonitrile containing 5% acetic acid (solvent A) and 80% acetonitrile (solvent B). The model of evaporative light scattering detector was YL9180 (Young-Lin, Anyang, Korea). Gradient elution was carried out as follows: 0 min, 0% B; 6 min, 30% B; 18 min, 50% B; 30 min, 100% B; and 37 min, 100% B.
데이터처리
Statistical significance was assessed using analysis of variance, followed by multiple comparison analysis with a Bonferroni adjustment. A p value of less than 0.
이론/모형
The cytotoxicity of FBG and PPD to HUVECs and HaCaT cells was assessed using the EZ-Cytox cell viability assay. In brief, cells were seeded at a density of 2 x 104 cells/mL in 96-well plates.
성능/효과
As shown in quantitative results, treatment with 25 μg/mL of FBG exhibited a statistically significant effect on wound closure at 2 d, 4 d, 6 d, and 8 d after treatments compared with control mice (Fig. 5B).
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