[논문]Comparison of in vitro ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration as a basal

Hamid, Muhammad Mahboob Ali; Park, Ha Young; Choi, Chang Weon

doi:10.7744/kjoas.20180026

Comparison of in vitro ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration as a basal 원문보기

Korean journal of agricultural science, v.45 no.3, 2018년, pp.419 - 427

Hamid, Muhammad Mahboob Ali (Department of Animal Resources, Daegu University) , Park, Ha Young (Department of Pathology, Busan Paik Hospital, Inje University College of Medicine) , Choi, Chang Weon (Department of Animal Resources, Daegu University)

Abstract ▼ AI-Helper

The present study was conducted to investigate the effect of different levels of Korean corn grain on in vitro ruminal fermentation with total mixed ration (TMR) as a basal feed. Three ruminal cannulated Holstein steers (Body Weight $479{\pm}33.0kg$) were used as rumen fluid donors. Treatments for in vitro fermentation were TMR only (control, 3.0 g), TMR substituted partially with high level (HC, TMR 1.5 and corn 1.5 g), and with low level of Korean corn grain (LC, TMR 2.25 and corn 0.75 g), respectively. To measure in vitro ruminal pH, gas production, ammonia N and volatile fatty acids (VFA), the in vitro fermentation incubation was triplicated at $39^{\circ}C$, 120 rpm for 0, 1, 3, 6, 12, 24 and 48 h, respectively. Mean ruminal pH was significantly lower (p < 0.05) for HC than control. Changes in rumen pH was rather similar between the groups till 6 h after incubation, but the lowest pH for HC (pH 5.10) appeared at 48 h compared with control and LC. Total gas production was tended (p < 0.09) to be higher and ammonia N was significantly lower (p < 0.05) for HC than control and LC. Total VFA was higher (p < 0.05) for HC and LC than control but no differences appeared between HC and LC. Overall, the present data indicate that feeding different levels of Korean domestic corn grain may lead to high and sustainable starch degradation in the rumen.

주제어

표/그림 (6)

표 Table 1. Chemical composition of experimental feeds (% of dry matter basis).
그림 Fig. 1. Changes in rumen pH after ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration (TMR) as a basal. Treatments were Control ● (TMR only, 3.0 g), HC ■ (TMR 1.5 + corn 1.5 g) and LC ▲ (TMR 2.25 + corn 0.75 g), respectively.
표 Table 2. Effect of different levels of Korean corn grains with total mixed ration on in vitro rumen fermentation parameters.
그림 Fig. 2. Changes in gas production after ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration (TMR) as a basal. Treatments were Control ● (TMR only, 3.0 g), HC ■ (TMR 1.5 + corn 1.5 g) and LC ▲ (TMR 2.25 + corn 0.75 g), respectively.
그림 Fig. 3. Changes in ammonia N after ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration (TMR) as a basal. Treatments were Control ● (TMR only, 3.0 g), HC ■ (TMR 1.5 + corn 1.5 g) and LC ▲ (TMR 2.25 + corn 0.75 g), respectively.
그림 Fig. 4. Changes in volatile fatty acid (VFA) after ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration (TMR) as a basal. Treatments were Control ● (TMR only, 3.0 g), HC ■ (TMR 1.5 + corn 1.5 g) and LC ▲ (TMR 2.25 + corn 0.75 g), respectively.

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문제 정의

In vitro systems have provided less expensive, more rapid alternatives and valuable data on kinetic aspects of starch digestion (Huntington, 1997). Therefore, this study conducted to investigate the effect of different levels of Korean corn grain on in vitro ruminal fermentation with total mixed ration (TMR), providing fundamental data of Korean corn in terms of rumen nutrition.

제안 방법

Water and mineral-vitamin block were allowed ad libitum. For in vitro rumen fermentation, treatments were TMR only (control, 3.0 g), TMR substituted partially with high level (HC, TMR 1.5 and corn 1.5 g), and low level of Korean corn grain (LC, TMR 2.25 and corn 0.75 g), respectively.
Changes in rumen pH after ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration (TMR) as a basal. Treatments were Control ● (TMR only, 3.0 g), HC ■ (TMR 1.5 + corn 1.5 g) and LC ▲ (TMR 2.25 + corn 0.75 g), respectively.
Changes in rumen pH after ruminal fermentation incubated with different levels of Korean corn grains with total mixed ration (TMR) as a basal. Treatments were Control ● (TMR only, 3.0 g), HC ■ (TMR 1.5 + corn 1.5 g) and LC ▲ (TMR 2.25 + corn 0.75 g), respectively.

이론/모형

Data for pH, gas production, ammonia N and VFA were analyzed using the general linear model procedure of the Statistical Analysis System Institute, Inc. (SAS, 2002). Differences among means were tested for significance (p < 0.

성능/효과

Incubation with corn grains statistically increased (p < 0.05) mean total VFA as compared to control (Table 2), and VFA patterns showed to be clearly higher for HC and LC than control during the entire trial except for the beginning of incubation at 0 and 1 h after incubation (Fig. 4).

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