Kim, Yangseon
(Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
,
Kang, In Jeong
(Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
,
Shin, Dong Bum
(Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
,
Roh, Jae Hwan
(Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
,
Heu, Sunggi
(Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
,
Shim, Hyeong Kwon
(Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
Fusarium graminearum causes the devastating plant disease Fusarium head blight and produces mycotoxins on small cultivated grains. To investigate the timeframe of F. graminearum infection during rice cultivation, a spore suspension of F. graminearum was applied to the rice cultivars Dongjin 1 and Na...
Fusarium graminearum causes the devastating plant disease Fusarium head blight and produces mycotoxins on small cultivated grains. To investigate the timeframe of F. graminearum infection during rice cultivation, a spore suspension of F. graminearum was applied to the rice cultivars Dongjin 1 and Nampyeongbyeo before and after the heading stage. The disease incidence rate was the highest (50%) directly after heading, when the greatest number of flowers were present, while only 10% of the rice infected 30 days after heading showed symptoms. To understand the mechanism of infection, an F. graminearum strain expressing green fluorescent protein (GFP) was inoculated, and the resulting infections were visually examined. Spores were found in all areas between the glume and inner seed, with the largest amount of GFP detected in the aleurone layer. When the inner part of the rice seed was infected, the pathogen was mainly observed in the embryo. These results suggest that F. graminearum migrates from the anthers to the ovaries and into the seeds during the flowering stage of rice. This study will contribute to uncovering the infection process of this pathogen in rice.
Fusarium graminearum causes the devastating plant disease Fusarium head blight and produces mycotoxins on small cultivated grains. To investigate the timeframe of F. graminearum infection during rice cultivation, a spore suspension of F. graminearum was applied to the rice cultivars Dongjin 1 and Nampyeongbyeo before and after the heading stage. The disease incidence rate was the highest (50%) directly after heading, when the greatest number of flowers were present, while only 10% of the rice infected 30 days after heading showed symptoms. To understand the mechanism of infection, an F. graminearum strain expressing green fluorescent protein (GFP) was inoculated, and the resulting infections were visually examined. Spores were found in all areas between the glume and inner seed, with the largest amount of GFP detected in the aleurone layer. When the inner part of the rice seed was infected, the pathogen was mainly observed in the embryo. These results suggest that F. graminearum migrates from the anthers to the ovaries and into the seeds during the flowering stage of rice. This study will contribute to uncovering the infection process of this pathogen in rice.
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제안 방법
Although the GFP-expressing strain showed a lower infection rate after the heading stage compared to the wild type, GFP was instrumental in discovering the infection sites in rice. Infection sites were studied by inoculation of the rice cultivars Dongjin 1 and Nampyeongbyeo at the following stages: before heading, directly after heading, and 10 days after heading.
Inoculation was performed with a 1 105/mL spore suspension of F. graminearum strains z3639 and Z39G418 at four developmental stages: before heading immediately after heading, 10 days after heading (milky stage), and 30 days after heading (mature stage).
In wheat, susceptibility to FHB is thought to be highest during the heading period, but such details of the infection method have not yet been experimentally determined in rice [16]. To investigate whether rice plants are also most susceptible to FHB infections during the heading stage, we examined the disease severity that resulted when inoculating pathogens before and after the rice heading period. The stages chosen reflect the fact that the flowering and grain-filling stages of rice begin within one to five days after heading and that grain filling is complete within three weeks.
In addition, the adaxial sides of glumes are accessible infection sites where spores can attach and spread further [20]. To investigate whether the FHB pathogen can proliferate in rice and to determine the susceptibilities of different rice growth stages, an F. graminearum strain expressing GFP in the wild-type background was used for further inoculation.
대상 데이터
The cultivars used in this experiment were Dongjin 1 and Nampyeongbyeo, which are heavily affected by FHB. The plants were cultivated in a greenhouse from seed sterilization until shortly before the heading stage; the plants were then inoculated with FHB pathogens.
graminearum wild-type strain z3639 and the GFPexpressing strain Z39G418. The strains were tested on two rice cultivars (Dongjin 1 and Nampyeongbyeo) and inoculated at different rice developmental stages before and after heading, over three replicates. Duncan’s test was used to determine significance at the 95% probability level.
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