[논문]First report of tetracycline-resistant Aeromonas veronii infection in Amur catfish (Silurus asotus) cultured in Korea

Kim, Hyo Yeon; Jun, Jin Woo; Lim, Se Ra; Park, Seon Young; Han, Jee Eun; Park, Se Chang; Kim, Ji Hyung

doi:10.14405/kjvr.2019.59.4.207

[국내논문] First report of tetracycline-resistant Aeromonas veronii infection in Amur catfish (Silurus asotus) cultured in Korea 원문보기

Korean journal of veterinary research = 대한수의학회지, v.59 no.4, 2019년, pp.207 - 211

Kim, Hyo Yeon (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology) , Jun, Jin Woo (Department of Aquaculture, Korea National College of Agriculture and Fisheries) , Lim, Se Ra (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology) , Park, Seon Young (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology) , Han, Jee Eun (Laboratory of Aquatic Biomedicine, College of Veterinary Medicine, Kyungpook National University) , Park, Se Chang (Laboratory of Aquatic Biomedicine, Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University) , Kim, Ji Hyung (Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology)

Abstract ▼ AI-Helper

Mass mortality in commercially cultured Amur catfish (Silurus asotus), showing symptoms of dermal ulcerations, occurred on a private farm in Mar 2019 in Korea. β-hemolytic bacteria were isolated from the ulcers and kidneys of the fish and identified as Aeromonas veronii. The isolate was resistant to tetracycline and possessed cytotoxic heat-labile enterotoxin (aerolysin/hemolysin). We investigated the genetic determinants associated with tetracycline resistance, and the isolate has been confirmed to simultaneously possess tetA and tetE genes. This is the first report on the occurrence of tetracycline-resistant A. veronii infection related to mass mortality in commercially cultured Amur catfish in Korea.

Keyword

표/그림 (4)

그림 Fig. 1. Aeromonas veronii infected juvenile Amur catfish (Silurus asotus) showing severe, open dermal ulcerations (red arrows) on the head, on the middle of the body, and on the dorsal regions of the fish. Scale bars indicate one centimeter.
표 Table 1. List of polymerase chain reaction primers used in this study
그림 Fig. 2. Maximum-likelihood tree based on the nucleotide sequences of rpoB in Aeromonas veronii strain AVNIH1 to some representative type strains of Aeromonas species and the outgroup Vibrio cholerae CECT 514T. The scale bar represents 0.05 nucleotide substitutions per site.
표 Table 2. Antibiotic resistance profiles of Aeromonas veronii strain AVN1H1

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

이론/모형

Antimicrobial susceptibility tests for the A. veronii isolate were performed using the disk diffusion method, using a total of 20 antimicrobial agents (Oxoid Ltd., UK), belonging to 9 different classes according to the guidelines of the Clinical and Laboratory Standards Institute [14]. According to the results of the disc diffusion test, the isolate was intermediate to imipenem (10 μg) and ciprofloxacin (10 μg), and resistant to tetracycline (30 μg) (Table 2).

성능/효과

The rpoB sequences were identical between our isolates and most similar to A. veronii ATCC35624T (98.4% nucleotide identity) among the species in the Aeromonadaceae (Fig. 2).
). The obtained results indicated that the isolates belong to the genus Aeromonas, but failed to discriminate to the species level. Therefore, the rpoB gene of the isolates that encodes the β-subunit of the DNA-dependent RNA polymerase was amplified and sequenced using the primers Pasrpob-L/Rpob-R [11] (Table 1).
veronii strain KC-1109, previously implicated in the mortality of adult catfish cultured in Korea [2], as well as ATCC 9071 and ATCC 35624^T, and the results are shown in Supplementary Table 1. The results showed that overall the biochemical phenotype of strain AVNIH1 was very similar to those of strain KC-1109 and ATCC 9071 but differed from ATCC 35624^T. Based on the positive results of the AMY test (oxidation of amygdalin), our isolate was distinct from strain KC-1109 and ATCC 9071.
veronii-associated fish diseases, and the aerolysin-mediated aerotaxis has been determined as a causal factor in MAS and EUS [13]. Based on these results, the isolated A. veronii strain AVNIH1 demonstrated a strong possibility of causing EUS in this case, and also possessed a pathogenic potential in other fish species.
To determine the genetic determinants associated with resistance to tetracycline, a multiplex PCR assay was conducted as previously described [9] to amplify the five tetracycline resistant genes (tetA to tetE) (Table 1). A total of two PCR amplicons were detected from A. veronii strain AVNIH1, and the sequencing analyses revealed that the isolate simultaneously possessed tetA and tetE as its genetic determinants associated with resistance to tetracycline. The newly identified tetA and tetE in A.
veronii strain AVNIH1, and the sequencing analyses revealed that the isolate simultaneously possessed tetA and tetE as its genetic determinants associated with resistance to tetracycline. The newly identified tetA and tetE in A. veronii strain AVNIH1 were identical to those were found in A hydrophila strain 23-C-23 plasmid (CP038466) and A. veronii strain MS-18-37 chromosome (CP033604), respectively.

참고문헌 (15)

Yu JH, Han JJ, Park KS, Park KH, Park SW. Edwardsiella tarda infection in Korean catfish, Silurus asotus, in a Korean fish farm. Aquacult Res 2009;41:19-26.

상세보기
Kim JD, Do JW, Choi HS, Seo JS, Jung SH, Jo HI, Park M, Lee N, Park SW. Pathological changes in cultured Korean catfish (Silurus asotus) artficially infected with Aeromonas veronii. Korean J Environ Biol 2013;31:486-492.

원문보기 상세보기
Janda JM, Abbott SL. Evolving concepts regarding the genus Aeromonas: an expanding Panorama of species, disease presentations, and unanswered questions. Clin Infect Dis 1998; 27:332-344.

상세보기
Cai SH, Wu ZH, Jian JC, Lu YS, Tang JF. Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from Chinese longsnout catfish (Leiocassis longirostris Gunther). Braz J Microbiol 2012;43:382-388.
Yi SW, You MJ, Lee HB, Shin GW. A case of Aeromonas veronii infection in Israeli carp (Cyprinus carpio): phylogenetic analysis and antimicrobial resistance. Korean J Vet Serv 2012;35:239-243.

원문보기 상세보기
Yu JH, Han JJ, Kim HJ, Kang SG, Park SW. First report of Aeromonas veronii infection in farmed Israeli carp Cyprinus carpio in Korea. J Fish Pathol 2010;23:165-176.

원문보기 상세보기
Joh SJ, Ahn EH, Lee HJ, Shin GW, Kwon JH, Park CG. Bacterial pathogens and flora isolated from farm-cultured eels (Anguilla japonica) and their environmental waters in Korean eel farms. Vet Microbiol 2013;163:190-195.

상세보기
Yi SW, You MJ, Cho HS, Lee CS, Kwon JK, Shin GW. Molecular characterization of Aeromonas species isolated from farmed eels (Anguilla japonica). Vet Microbiol 2013;164:195-200.

상세보기
Kim JH, Hwang SY, Son JS, Han JE, Jun JW, Shin SP, Choresca C Jr, Choi YJ, Park YH, Park SC. Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea. J Vet Sci 2011;12:41-48.

상세보기
Han JE, Kim JH, Choresca CH Jr, Shin SP, Jun JW, Chai JY, Park SC. Prevalence of tet gene and complete genome sequencing of tet gene-encoded plasmid (pAHH01) isolated from Aeromonas species in South Korea. J Appl Microbiol 2012;112:631-638.

상세보기
Korczak B, Christensen H, Emler S, Frey J, Kuhnert P. Phylogeny of the family Pasteurellaceae based on rpoB sequences. Int J Syst Evol Microbiol 2004;54:1393-1399.

상세보기
Aravena-Roman M, Inglis TJ, Riley TV, Chang BJ. Distribution of 13 virulence genes among clinical and environmental Aeromonas spp. in Western Australia. Eur J Clin Microbiol Infect Dis 2014;33:1889-1895.

상세보기
Foysal MJ, Momtaz F, Ali MH, Siddik MA, Chaklader MR, Rahman MM, Prodhan MS, Cole A. Molecular characterization and interactome analysis of aerolysin (aer) gene from fish pathogen Aeromonas veronii: the pathogenicity inferred from sequence divergence and linked to histidine kinase (cheA). J Fish Dis 2019;42:465-475.

상세보기
Clinical and Laboratory Standards Institute (CLSI). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria. 3rd ed. CLSI guideline M45. CLSI, Wayne, 2015.
Rhee JY, Jung DS, Peck KR. Clinical and therapeutic implications of Aeromonas bacteremia: 14 years nation-wide experiences in Korea. Infect Chemother 2016;48:274-284.

상세보기

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증