[논문]The Processed Radish Extract Melanogenesis in Humans and Induces Anti-Photoaging Effects in Ultraviolet B-Induced Hairless Mouse Model

Kim, Hyun-Kyoung

doi:10.17703/ijact.2019.7.4.125

[국내논문] The Processed Radish Extract Melanogenesis in Humans and Induces Anti-Photoaging Effects in Ultraviolet B-Induced Hairless Mouse Model 원문보기

The International journal of advanced culture technology, v.7 no.4, 2019년, pp.125 - 136

Kim, Hyun-Kyoung (Department of Food Science and Engineering, Seowon University)

Abstract ▼ AI-Helper

The radish skin and radish greens are an edible part of the radish. But they are removed before eating the radish and used as a byproduct or an animal feed material because of their tough and rough texture. Melanin is a pigment that gives colour to our skin. But increased production of melanin can turn into benign or malignant tumours. These days due to global warming, the amount of Ultra violet (UVB) rays has been extensively increased with sunlight. Due to this, a phenomenon called exogenous photo aging is widely observed for all skin colour and types. As a result of this phenomenon, a set of enzymes called matrix metalloproteinases (MMP's) that serves as degradation enzymes for extracellular matrix proteins mainly collagen is increased, causing depletion in collagen and resulting in early wrinkles formation. Therefore in our study we used the murine melanoma cell line B16/F10 to study the melanogenesis inhibition by Heated radish extract (HRE) in vitro and we used HRM-2 hair less mice exposed to artificial UVB for checking the efficacy of Heated radish extract in vivo. Furthermore, we prepared a 3% Heated radish extract (HRE) cream and checked its effects on human skin. Our results have clearly demonstrated that Heated radish extract (HRE) have potently suppressed the tyrosinase activity and melanin production in B16/F10 cells. It had also reduced the expression of components involved in melanin production pathway both transcriptionally and transitionally. In in vivo studies, HRE had potently suppressed the expression of MMP's and reduced the wrinkle formation and inhibited collagen degradation. Moreover, on human skin, ginseng cream increased the resilience, skin moisture and enhanced the skin tone. Therefore in light of these findings, we conclude that HRE is an excellent skin whitening and antiaging product.

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표/그림 (8)

표 Table 1. Oligonucleotide sequences of primers/probe used for qRT-PCR and Real-Time PCR.
그림 Figure 1. Inhibition of mushroom tyrosinase and production of melanin by HRE.
그림 Figure 2. Depressed expression of MITF pathway genes by HRE.
그림 Figure 3. Effects of HRE on body weight and food intake. To measure body weight and food intake, HRM-2 mice were exposed to UVB for 5 weeks with the HRE orally and positive control treatment on skin. Food intake and body weight was taken daily during 5 weeks.
그림 Figure 4. Analysis of wrinkle-related genes expression in UVB irradiated skin damage induction model.
그림 Figure 5. Effects of HRE on Melanogenesis in Skin Damage Induced Conditional Model by UVB Irradiation.
그림 Figure 6. Fig.6. Effects of HRE on wrinkle depth and formation in HRM-2 mice.
그림 Figure 7. Effects of HRE on epithelial thickness and collagen fiber changes in HRM-2 mice UVB

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