[논문]생쥐 연골세포에 Arnica montana 처리에 따른 COX-2 발현과 PGE2 분비 비교

김윤규; 여명구

doi:10.15207/jkcs.2019.10.2.331

생쥐 연골세포에 Arnica montana 처리에 따른 COX-2 발현과 PGE2 분비 비교
Treatment with ultra-dilutions of Arnica montana increases COX-2 expression and PGE2 secretion in mouse chondrocytes 원문보기

한국융합학회논문지 = Journal of the Korea Convergence Society, v.10 no.2, 2019년, pp.331 - 337

김윤규 (울산대학교 의과대학) , 여명구 (남부대학교 통합의료학과)

초록
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연구목적 : 4x, 30x, 30c, and 200c 농도의 동종약물 Arnica montana (A. montana)를 1차 배양된 생쥐 연골세포에 적용하여 염증관련 인자의 변화를 관찰하고자 하였다. 연구방법 : 본 연구는 collagen type II (Coll-2), cyclooxygenase-2 (COX-2) 발현 그리고 prostaglandin 2 (PGE2) 분비에 대해 조사하였다. 결과: 4x, 30x 그리고 30c의 A. montana를 처리하였을 때, Coll-2의 mRNA 발현이 감소하였으며, 30x A. montana의 경우 COX-2 mRNA의 발현이 증가하였다. 또한 COX-2 단백질 발현은 30x와 30c의 A. montana 처리 시 증가함을 보였다. PGE2 분비 또한 30c에서 증가함을 관찰하였다. 결론 : A. montana 처리에 따라 생쥐의 연골세포의 분화 억제를 확인하였으며, 염증관련 인자인 COX-2 및 PGE2의 발현이 증가함을 확인하였다.

Abstract ▼ AI-Helper

Objective: We studied the effects of 4x, 30x, 30c, and 200c homeopathic dilutions of A. montana on inflammation in primary cultured mouse chondrocytes. Methods: Examined expression of Coll-2 and COX-2, and secretion of PGE2. Results: Treatment with 4x, 30x, and 30c A. montana decreased mRNA expression of Coll-2 and 30x A. montana increased mRNA expression of COX-2, while treatment with 30x and 30c A. montana increased protein expression of COX-2. Treatment with the 30c A. montana increased release of PGE2. Conclusion: Treatment with A. montana induces dedifferentiation and inflammatory responses, including increased COX-2 expression and PGE2 secretion.

주제어

표/그림 (4)

그림 Fig. 1. Treatment with homeopathic dilutions of A. montana does not affect proliferation of chondrocytes or elicit cytotoxic effects.
그림 Fig. 2. Treatment with the 30x homeopathic dilution of A. montana increases mRNA expression of COX-2 in chondrocytes.
그림 Fig. 3. Treatment with 4x, 30x, and 30c homeopathic dilutions of A. montana increases protein expression of COX-2 in chondrocytes.
그림 Fig. 4. Treatment with the 30c homeopathic dilution of A. montana increases release of PGE2 by chondrocytes.

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문제 정의

This study investigated the effects of A. montana on primary cultured mouse chondrocytes. To determine the effects of homeopathic dilutions of A.

제안 방법

PCR was performed using a GeneAmp PCR System 9700 (Applied Biosystems, Carlsbad, CA, USA) and AmpliTaq DNA polymerase (PE Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions.
The effect of A. montana on proliferation of primary cultured mouse chondrocytes and its cytotoxic effects were evaluated using the MTT and LDH assays, respectively. Cells were analyzed following treatment with 0.
Previous studies suggested that a broad spectrum of inflammation mediators regulate COX-2 expression[8] and that PGE2, the major COX-2 product, is upregulated in various cell lines[17,18]. The results of the present study are consistent with previous findings demonstrating a correlation between COX-2 expression and PGE2 production.

데이터처리

Values are presented as the mean ± standard error of the mean (SEM). Data were statistically analyzed using the independent samples t-test and an analysis of variance. p<0.

이론/모형

5), 150 mM NaCl, 1 mM EDTA, 10 mM NaF, 1 mM pyrophosphate, and 2 mM Na₃VO₄) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) on ice for 10min. The protein concentration was determined using the Bradford method (Bio-Rad Laboratories, Hercules, CA, USA). Proteins were separated by SDS-PAGE.

후속연구

montana, in vitro and provide a basis for future research. Further studies are required to investigate the effectiveness of A. montana in vivo and the underlying mechanism.
montana increases mRNA and protein expression of COX-2 in primary cultured mouse chondrocytes and thereby increases PGE2 secretion. These findings support the effectiveness of homeopathic remedies, particularly A. montana, in vitro and provide a basis for future research. Further studies are required to investigate the effectiveness of A.

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