[논문]전통적인 한방 처방 경옥고의 면역 증강 효과

노성수; 이원화; 김경민; 나민균; 배종섭

doi:10.6116/kjh.2019.34.2.41.

전통적인 한방 처방 경옥고의 면역 증강 효과
Immune-enhancing effects of a traditional herbal prescription, Kyung-Ok-Ko 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.34 no.2, 2019년, pp.41 - 47

노성수 (대구한의대학교 한의과대학 본초약리학교실) , 이원화 (경북대학교 약학대학 약학과) , 김경민 (경북대학교 농업생명과학대학 응용생명과학부) , 나민균 (충남대학교 약학대학 약학과) , 배종섭 (경북대학교 약학대학 약학과)

Abstract ▼ AI-Helper

Objectives : A traditional herbal prescription, Kyung-Ok-Ko (KOK), has long been used in oriental medicine as an invigorant for age-related diseases, such as amnesia and stroke. However, the beneficial value of KOK for immune responses is largely unknown. Based on the above mentioned effects of KOK, other previous reports, and its use in traditional medicine, we hypothesized that KOK displays beneficial effects against methotrexate (MTX)-induced immune suppression. Methods : We investigated the effects of KOK (0.6 g/kg/day, oral (p.o.)) on deteriorated immunity caused by MTX (2 mg/kg/day, p.o.) in an immune suppression mouse model. MTX was fed to mice once a day for 7 days. After the immune responses of the mice deteriorated by MTX treatment, KOK in water was fed to the mice once a day for 14 days. We then measured the expression levels of various cytokines, such as T helper cell (Th1, Th2) cytokines, and the number of immune cells, such as spleen T cells, B cells, and macrophages. Results : The data showed that MTX decreased Th1 profiles (interferon $(IFN)-{\gamma}$, interleukin (IL)-2, IL-12) and the number of immune cells, and increased Th2 profiles (IL-4, IL-5, IL-13), which were normalized significantly by post-administration of KOK. However, there was no significant difference in body-weight gain between MTX- and KOK-treated mice. Conclusion : These results indicate that KOK has immune-enhancing functions and reduces immunotoxicity of MTX, suggesting that supplementation with KOK will improve immune responses clinically and be useful for the prevention of immune-related diseases.

주제어

표/그림 (4)

그림 Fig. 1. Effects of KOK on Th1 cytokine levels. Mice were administered MTX (2 mg/kg/day, p.o.) for 1 week, followed by VC (p.o.), KOK (0.6 g/kg, p.o.), or RG extract (45 mg/kg/day, p.o.) for two weeks. Concentrations of interferon (IFN)-γ(A), interleukin (IL)-2(B), and IL-12(C) (pg/mL) in serum were measured by mouse cytokine 10-Plex Panel kit.
표 Table 1. Effects of MTX or KOK on mouse body weight and food intake.
그림 Fig. 2. Effects of KOK on Th2 cytokine levels. Mice were administered MTX (2 mg/kg/day, p.o.) for 1 week, followed by VC (p.o.), KOK (0.6 g/kg, p.o.), or RG extract (45 mg/kg/day, p.o.) for two weeks. Concentrations of interleukin (IL)-4(A), IL-5, and IL-13 (pg/mL) in serum were measured by mouse cytokine 10-Plex Panel kit or ELISA.
그림 Fig. 3. Effects of KOK on splenic cellularity. Mice were administered MTX (2 mg/kg/day, p.o. for 1 week, followed by VC (p.o.), KOK (0.6 g/kg, p.o.), or RG extract (45 mg/kg/day, p.o.) for two weeks. Markers of each cell were stained and the number of spleenocyte(A), T cell(B), B cell(C), and Macrophage(D) was analyzed using a FACScan^TM flow cytometer .

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문제 정의

In addition, some studies have examined the effects of KOK on age-related disorders, as well as the biological properties of KOK, including its antioxidant, anti-inflammatory, anti-fatigue, and immunological activities^{12, 13)}. Therefore, the aim of the present study was to investigate whether KOK affected the pattern of Th1 and Th2 cytokine secretion and regulated the Th1/Th2 balance.

제안 방법

) for two weeks. Markers of each cell were stained and the number of spleenocyte(A), T cell(B), B cell(C), and Macrophage(D) was analyzed using a FACScan^TM flow cytometer . Results are presented as means ± SD (n = 10).
To investigate the role of KOK in the secretion into the blood of Th1 cytokines, KOK in water was fed to the mice once a day for 14 days after immune responses of the mice deteriorated by MTX treatment. We then measured the expression levels of Th1 cytokines in serum, such as IFN-γ, IL-2, and IL-12.

데이터처리

The Student’s t-test was used to calculate significant differences and statistical significance was accepted at p-values < 0.05.

성능/효과

According to our results, decreased expression of Th1 cytokines, such as IFN-γ, IL-2, and IL-12, by MTX was recovered by KOK, while increased expression of Th2 cytokines, such as IL-4, IL-5, and IL-13, by MTX was decreased by KOK.
Data showed that when KOK (0.6 g/kg, p.o.) was added after MTX treatment, decreased IFN-γ, IL-2, and IL-12 by MTX was recovered significantly by KOK treatment (Fig. 1).
In conclusion, our results suggest that KOK regulates Th1 and Th2 immune parameters of vascular function. Noting that the imbalance between Th1 and Th2 systems and the disordered splenic cellularity by MTX could be coordinated by KOK, our findings also present novel evidence about the potential clinical implications of KOK treatment not only in normal vascular immune function but also in preventing immune-related diseases.

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