[논문]Anti-inflammatory Effect of Branches Extracts from Quercus mongolica in LPS-induced RAW264.7 Cells

Eo, Hyun Ji; Park, Youngki; Kang, Jin Taek; Park, Gwang Hun

doi:10.7732/kjpr.2019.32.6.698

[국내논문] Anti-inflammatory Effect of Branches Extracts from Quercus mongolica in LPS-induced RAW264.7 Cells 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.32 no.6, 2019년, pp.698 - 704

Eo, Hyun Ji (Forest Medicinal Resources Research Center, National Institute of Forest Science) , Park, Youngki (Forest Medicinal Resources Research Center, National Institute of Forest Science) , Kang, Jin Taek (Division of Forest Industry Research, National Institute of Forest Science) , Park, Gwang Hun (Forest Medicinal Resources Research Center, National Institute of Forest Science)

Abstract ▼ AI-Helper

Quercus mongolica (QM), which belongs to fagaceae, is one of the oak native to Korea. We evaluated the anti-inflammatory effect of branches extracted with 70% ethanol of QM (QM-B) and elucidated the potential signaling pathway in LPS-induced RAW264.7 cells. The QM-B showed anti-inflammatory activity through inhibition of NO production. The QM-B dose-dependently suppressed NO production by inhibiting iNOS, COX-2 and IL-6 expression in LPS-induced RAW264.7 cells. The QM-B inhibited the degradation and phosphorylation of IκB-α and NF-κB activation. The QM-B suppressed the phosphorylation of p38 and ERK1/2. Also, the QM-B increased HO-1 expression. These results suggested that QM-B may utilize anti-inflammatory activity by suppressing NF-κB and MAPK signaling pathway and inducing HO-1 expression indicated that the QM-B can be used as a natural anti-inflammatory drugs.

주제어

표/그림 (5)

표 Table 1. The primer sequences used in this study
그림 Fig. 1. The effect of QM-B on NO production and iNOS, COX-2 and IL-6 expression in LPS-stimulated RAW264.7 cells.
그림 Fig. 2. The effect of QM-B on NF-κB signaling activation in LPS-stimulated RAW264.7 cells.
그림 Fig. 3. The effect of QM-B on MAPK signaling activation in LPS-stimulated RAW264.7 cells.
그림 Fig. 4. Effect of QM-B on HO-1 expression in RAW264.7 cells.

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제안 방법

To further investigate whether decrease of NF-κB activation by QM-B treatment is associated with the regulation of p38 and ERK1/2 activation, we evaluated the effects of QM-B on phosphorylation of p38 and ERK1/2 in LPS-induced RAW264.7 cells.

대상 데이터

RAW264.7 cells were purchased American Type Culture Collection (ACTT, Virginia, USA) and grown in Dulbecco's Modified Eagle (Lonza, Walkerscille, MD, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (100 units/mL) and streptomycin (100 ㎍/mL) under an atmosphere of 5% CO2 at 37℃.
Dulbecco’s Modified Eagle medium (DMEM)/F-12 1:1 Modified medium (DMEM/F-12) was purchased from Lonza (Walkersville, MD, USA). lipopolysaccharide (LPS) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against phospho-IκB-α, IκB-α, phospho-extracellular signal-regulated kinase1/2 (p-ERK 1/2), ERK1/2, phospho-p38 (p-p38), p38, HO-1 and β-actin were purchased from Cell Signaling technology (Bervely, MA, USA).

데이터처리

Statistical analysis was performed with one-way ANOVA followed by Dunnett's test.

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