[논문]Renal protective effects of zingerone in a mouse model of sepsis

Lee, Bong-Seon; Lee, Changhun; Yang, Sumin; Ku, Sae-Kwang; Bae, Jong-Sup

doi:10.5483/bmbrep.2019.52.4.175

[국내논문] Renal protective effects of zingerone in a mouse model of sepsis 원문보기

BMB reports, v.52 no.4, 2019년, pp.271 - 276

Lee, Bong-Seon (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University) , Lee, Changhun (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University) , Yang, Sumin (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University) , Ku, Sae-Kwang (Department of Histology and Anatomy, College of Korean Medicine, Daegu Haany University) , Bae, Jong-Sup (College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences, BK21 Plus KNU Multi-Omics based Creative Drug Research Team, Kyungpook National University)

Abstract ▼ AI-Helper

Zingerone (ZGR), a phenolic alkanone isolated from ginger, has been reported to possess pharmacological activities such as anti-inflammatory and anti-apoptotic effects. This study was initiated to determine whether ZGR could modulate renal functional damage in a mouse model of sepsis and to elucidate the underlying mechanisms. The potential of ZGR treatment to reduce renal damage induced by cecal ligation and puncture (CLP) surgery in mice was measured by assessment of serum creatinine, blood urea nitrogen (BUN), lipid peroxidation, total glutathione, glutathione peroxidase activity, catalase activity, and superoxide dismutase activity. Treatment with ZGR resulted in elevated plasma levels of BUN and creatinine, and of protein in urine in mice with CLP-induced renal damage. Moreover, ZGR inhibited nuclear $factor-{\kappa}B$ activation and reduced the induction of nitric oxide synthase and excessive production of nitric acid. ZGR treatment also reduced the plasma levels of interleukin-6 and tumor necrosis $factor-{\alpha}$, reduced lethality due to CLP-induced sepsis, increased lipid peroxidation, and markedly enhanced the antioxidant defense system by restoring the levels of superoxide dismutase, glutathione peroxidase, and catalase in kidney tissues. Our study showed renal suppressive effects of zingerone in a mouse model of sepsis, suggesting that ZGR protects mice against sepsis-triggered renal injury.

Keyword

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문제 정의

The purpose of this study was to test the beneficial effect of ZGR, an active compound isolated from C. tricuspidata on renal injury in CLP-induced septic mice. Data showed that treatment with ZGR after CLP surgery dramatically improved CLP-induced renal damages.

제안 방법

As controls, sham-operated animals were used: in these mice, the cecum was exposed, but not ligated or punctured, and then returned to the abdominal cavity. Animals were randomly divided into 7 treatment groups (n = 10 each): sham-operated control; ZGR-only (0.72 mg/kg body weight) in 0.5% DMSO; CLP surgery only; and CLP ＋ ZGR (0.07, 0.14, 0.29, or 0.72 mg/kg body weight). ZGR was intravenously injected at 12 h after CLP and again at 50 h after CLP.

데이터처리

Statistical relevance was determined by one-way analysis of variance (ANOVA) and Tukey’s post-hoc test.

이론/모형

The homogenate was stored at −80^oC until analyzed in the various biochemical assays. Protein concentrations were determined using the Bradford assay.

성능/효과

Based on the current findings that ZGR reduced the levels of both inflammatory regulators, IL-6 and TNF-α, ZGR could affect the crucial point of pathogenesis in the inflammation diseases.
tricuspidata on renal injury in CLP-induced septic mice. Data showed that treatment with ZGR after CLP surgery dramatically improved CLP-induced renal damages. Moreover, the elevated levels of TNF-α, IL-6, NO, iNOS, and MPO by CLP was inhibited by ZGR.

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