Oyinade A., David
(Department of Plant Science and Biotechnology, Federal University Oye-Ekiti)
,
Oluwole, Osonubi
(Botany Department, University of Ibadan)
,
Jacob, Oyetunji Olusola
(Botany Department, University of Ibadan)
Digitaria exilis L. is an under-utilized crop with high nutritional and medicinal values. It thrives in and is well-adapted to arid areas with low soil nutrients. Using biochemical markers, this study investigates the mechanisms by which D. exilis responds to osmotic stress. Three accessions Dinat I...
Digitaria exilis L. is an under-utilized crop with high nutritional and medicinal values. It thrives in and is well-adapted to arid areas with low soil nutrients. Using biochemical markers, this study investigates the mechanisms by which D. exilis responds to osmotic stress. Three accessions Dinat Iburua (DIN), Jakah Iburua (JAK) and Jiw Iburua (JIW) were collected from National Cereal Research Institute, Niger State. Two accessions, NG/11/JD/061 and NG/11/JD/062 were also collected from National Centre for Genetic Resources and Biotechnology, Ibadan. Murashige and Skoog medium of approximately 1.2 L was supplemented with polyethylene glycol 6000 to create osmotic pressures of -9.29, -13.93, -20.13, -26.32, -32.51, and 0 MPa (control). Sterilized seeds were inoculated in the medium and placed in the growth room for 4 weeks. Proline accumulation was significantly high in all JAK plants under osmotic stress. Proline and ascorbate peroxidase (p50% of OH-, H2O2, and NO radicals were scavenged. However, other accessions including DIN, NG061, NG062, and JIW showed variations in their responses to different levels of osmotic stress, although not significant. Therefore, JAK possesses a well-equipped free radical quenching system that is protected by the accumulation of the osmolyte proline; therefore, accession JAK is considered osmotolerant. CAT and superoxide dismutase activities were osmostabilized against oxidative stress by proline.
Digitaria exilis L. is an under-utilized crop with high nutritional and medicinal values. It thrives in and is well-adapted to arid areas with low soil nutrients. Using biochemical markers, this study investigates the mechanisms by which D. exilis responds to osmotic stress. Three accessions Dinat Iburua (DIN), Jakah Iburua (JAK) and Jiw Iburua (JIW) were collected from National Cereal Research Institute, Niger State. Two accessions, NG/11/JD/061 and NG/11/JD/062 were also collected from National Centre for Genetic Resources and Biotechnology, Ibadan. Murashige and Skoog medium of approximately 1.2 L was supplemented with polyethylene glycol 6000 to create osmotic pressures of -9.29, -13.93, -20.13, -26.32, -32.51, and 0 MPa (control). Sterilized seeds were inoculated in the medium and placed in the growth room for 4 weeks. Proline accumulation was significantly high in all JAK plants under osmotic stress. Proline and ascorbate peroxidase (p50% of OH-, H2O2, and NO radicals were scavenged. However, other accessions including DIN, NG061, NG062, and JIW showed variations in their responses to different levels of osmotic stress, although not significant. Therefore, JAK possesses a well-equipped free radical quenching system that is protected by the accumulation of the osmolyte proline; therefore, accession JAK is considered osmotolerant. CAT and superoxide dismutase activities were osmostabilized against oxidative stress by proline.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
Larger polyethylene glycol molecules such as poly-ethylene glycol 6000 are more useful for simulating soil drying5 . The study therefore aimed at the biochemical mechanisms undertaken by D. exilis to survive osmotic stress.
대상 데이터
Five accessions of D. exilis were used in the studies, which were Dinat Iburua (DIN), Jakah Iburua (JAK), Jiw Iburua (JIW), NG/JD/06/11/062 (NG062) and NG/JD/06/11/061 (NG061). Three accessions DID, JAK and JIW were obtained from National Cereal Research Institute, Badeggi, Niger State, Nigeria, while the other two accessions NG061 and NG062 were obtained from National Centre for Genetic Resources and Biotechnology (NACRAB), Moor Plantation Ibadan, Nigeria.
데이터처리
Values with the same letters in each column are not significantly different from each other at Duncan’s multiple range test of P < 0.05.
이론/모형
The extraction of leaf pigments was performed with 75% ethanol, and the absorbance at 663 and 645 nm were measured with a spectrophotometer. The chlorophyll a, chlorophyll b, and total chlorophyll quantities were calculated according to the method of Arnon. The pigment concentrations were expressed as µg/ml.
Total amount of lipid peroxidation products present in the plant samples was estimated by the thiobarbituric acid (TBA) method which measures the malondialdehyde (MDA) reactive products according to the method of Ohkawa.
성능/효과
MDA is negatively correlated to OH, H 2 O 2 and NO (Table 6). Accession JAK had 85% osmotic tolerant level which was higher than the other accessions followed by NG061 (65%), DIN (55%), NG061 (48%) and JIW (47%). Tolerant level of D.
참고문헌 (22)
Adoukonou-Sagbadja H, Wagner C, Dansi A, Ahlemeyer J, Dainou O, Akpagana K and Friedt, W 2007 Genetic diversity and population differentiation of traditional D. exilis millet (Digitaria spp.) accessions from different agro-ecological zones of West Africa. Theoretical and Applied Genetics, 115(7):917-931
Coelho DLM, Agostini EAT, Guaberto LM, Machado Neto, NB and Custodio CC 2010 Differential Protein Expression during Germination as a Result of a Water Deficit Associated with Variable Osmotic Pressure in Snap- Beans. Acta Scientiarum Agronomy, 32:491-499
Ferreira GS, Torres SB and Costa ARFC 2007 Germination and Initial Development Stage of Melon Seedlings at Different Levels of Salinity of Irrigation Water. Caatinga, 20:181-185
Custodio CC, Salomao GR and Machado Neto NB 2009 Water Deficiency during Bean Seed Germination Induced by Different Osmotic Solutions. Revista Ciencia Agronomica, 40:617-623
Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts, polyphenoloxidases in Beta vulgaris. Plant Physiology 24:1-15
Marcocci L, Packer L. and Droy-Lefai, M.T. 1994 Antioxidant action of Ginkgo biloba extracts. Methods Enzymol, 234:462-75
Halliwell B 1990. How to characterize a biological antioxidant. FreeRadical Res. Commun, 9:1-32
Ruch RJ, Cheng SJ and Klaunig JE 1989 Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen, 10:1003-1008
Kumar J and Abbo S 2001 Genetics of flowering time in chickpea and its bearing on productivity in the semi-arid environments. Adv. Agron. 72:107-138
Morgan PW 1990. Effects of abiotic stresses on plant hormone systems, in: Stress Responses in plants: adaptation and acclimation mechanisms, Wiley-Liss, Inc., pp 113-146
Taiz L and Zeiger E 2006 Plant Physiology, 4th Ed., Sinauer Associates Inc. Publishers, Massachusetts
Montillet JL, Chamnongpol S, Rusterucci C, Dat J, van de Cotte B, Agnel JP, Battesti C, Inze D, Van Breusegem F and Triantaphylides C 2005 Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves, Plant Physiol. 138:1516-1526
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.