[논문]OVA-유도 쥐 모델에서 기도 세포 침윤에 대한 KM1701의 억제효과

임순민; 최한석; 김상백; 김예진; 강기성; 신명숙; 김경준; 황귀서; 구본암

doi:10.6114/jkood.2019.32.2.001

OVA-유도 쥐 모델에서 기도 세포 침윤에 대한 KM1701의 억제효과
Inhibitory Effects of KM1701 on Airway Cell Infiltration in OVA-Induced Mouse Model 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.32 no.2, 2019년, pp.1 - 10

임순민 (한국콜마 생명과학연구소) , 최한석 (한국콜마 생명과학연구소) , 김상백 (한국콜마 생명과학연구소) , 김예진 (한국콜마 생명과학연구소) , 강기성 (가천대학교 한의과대학) , 신명숙 (가천대학교 한의과대학) , 김경준 (가천대학교 한의과대학) , 황귀서 (가천대학교 한의과대학) , 구본암 (한국콜마 생명과학연구소)

Abstract ▼ AI-Helper

Objectives : The objective of present study is to evaluate anti-inflammatory and anti-allergic effects of Perilla(Perilla frutescens; Labiatae, PF), the roots of Peucedanum praeruptorum(PP) and the root of Scutellaria baicalensis(SB) in vitro and anti-asthmatic effects of mixture of PF, PP and SB(PS) on ovalbumin (OVA)-induced asthma in BALB/c mice. Methods : Anti-inflammatory and anti-allergic effects were observed on the lipopolysaccharide(LPS) treated RAW 264.7 cells through Nitric Oxide(NO) production and RBL-2H3 cells through ${\beta}$-hexosaminidase. Anti-asthmatic effects were observed on the number of inflammatory cells in bronchoalveolar lavage fluid(BALF) and the level of IgE in serum on OVA-induced BALB/c mice. Results : The treatment of PF, PP and SB(12.5, 25, 50, $100{\mu}g/m{\ell}$) resulted in a significant inhibition of NO production in RAW 264.7 cells and mast cell degranulation in RBL-2H3 cells. Oral administration of PS(400mg/kg/day) resulted in a significant reduction in the numbers of eosinophils in BALF and level of IgE in serum. Conclusion : The oral administration of PS is effective in ameliorating the eosinophilic infiltration in vivo and thus can be a good therapeutic candidate for allergic asthma.

주제어

표/그림 (3)

그림 Fig. 1. Inhibitory Effects of PF, PP and SB on Release of NO Production from LPS-Induced RAW 264.7 Cells and β-hexosaminidase from IgE-Sensitized RBL-2H3 Cells
그림 Fig. 2. Effects of PS1-5 on BAL Fluid Cell Infiltration and IgE Levels of OVA-Challenged Mice
그림 Fig. 3. Effects of KM1701 on Release of β-hexosaminidase from IgE-Sensitized RBL-2H3 Cells and BAL Fluid Cell Infiltration of OVA-Challenged Mice

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대상 데이터

RAW 264.7 mouse macrophage cells and RBL-2H3 rat basophilic leukemia cells were purchased from the American Type Culture Collection(ATCC; Manassas, VA, USA) and cultured in DMEM supplemented with 10% FBS, 100 U/㎖ penicillin and 100㎍/㎖ streptomycin at 37℃ in a humidified 5% CO2 atmosphere.

데이터처리

Animal studies were evaluated by the Student’s t-test.
NO, MTT and β-hexosaminidase assays were evaluated by one-way analysis of variance(ANOVA) analysis followed by the Tukey’s post hoc test.

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