[논문]Antidiabetic Activities of Korean Red Pine (Pinus densiflora) Inner Bark Extracts

Min, Hee-Jeong; Kim, Eun-Ji; Shinn, Seong-whan; Bae, Young-Soo

doi:10.5658/wood.2019.47.4.498

Antidiabetic Activities of Korean Red Pine (Pinus densiflora) Inner Bark Extracts 원문보기

목재공학 = Journal of the Korean wood science and technology, v.47 no.4, 2019년, pp.498 - 508

Min, Hee-Jeong (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University) , Kim, Eun-Ji (Regional Strategic Industry Innovation Center, Hallym University) , Shinn, Seong-whan (Department of Advanced Materials and Chemical Engineering, Halla university) , Bae, Young-Soo (Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University)

Abstract ▼ AI-Helper

This study was carried out to investigate the potential of Korean red pine (Pinus densiflora) inner bark extracts as an antidiabetic agent. The ethyl acetate soluble fraction of the bark extracts was chromatographed on a Sephadex LH-20 column to yield five compounds, which structures were elucidated by NMR spectroscopy. The isolated compounds were (+)-catehin, (-)-epicatechin, taxifolin, taxifolin-3'-O-${\beta}$-D-(+)-glucose and $\tilde{n}$-courmaric acid. The antidiabetic activity of the different fractions, including the crude extracts and isolated compounds, was evaluated by ${\beta}$-cells insulin secretion and glucose uptake in skeletal muscle cells. The insulin secretion was 128% for taxifolin at $25{\mu}g/mL$. However, the other samples had no effect on this test. For the glucose uptake activity assay, $1{\mu}M$ insulin and 2 mM metformin were used as controls. Both the crude extract and taxifolin showed relatively low activity values, but the other samples yielded glucose uptake values over 260%. ${\rho}$-courmaric acid showed the highest uptake (270%). The results confirmed that Korean red pine extracts may be used as a hypoglycemic agent.

주제어

표/그림 (35)

$Table 1. Weight of each fraction of Korean red pine inner bark extracts$ 표 Table 1. Weight of each fraction of Korean red pine inner bark extracts
그림 Fig. 1. Chemical structures of isolated compounds.
표 Table 2. Weight of the isolated compounds
그림 Fig. 2. Effects of crude on the viability of HIT-T15 cells.
$Fig. 3. Effects of EtOAc soluble fraction on the viability of HIT-T15 cells.$ 그림 Fig. 3. Effects of EtOAc soluble fraction on the viability of HIT-T15 cells.
그림 Fig. 5. Effects of (+)-catechin on the viability of HIT-T15 cells.
그림 Fig. 6. Effects of (-)-epicatechin on the viability of HIT-T15 cells.
$Fig. 4. Effects of H2O soluble fraction on the viability of HIT -T15 cells.$ 그림 Fig. 4. Effects of H₂O soluble fraction on the viability of HIT -T15 cells.
그림 Fig. 7. Effects of taxifolin on the viability of HIT-T15 cells.
그림 Fig. 8. Effects of taxifolin-3′-O-β-D-(+)-glucose on the viability of HIT-T15 cells.
그림 Fig. 9. Effects of ρ-coumaric acid on the viability of HIT-T15 cells.
그림 Fig. 10. Insulin secretion of HIT-T15 treated with the crude.
$Fig. 12. Insulin secretion of HIT-T15 treated with the H2O soluble fraction.$ 그림 Fig. 12. Insulin secretion of HIT-T15 treated with the H₂O soluble fraction.
그림 Fig. 14. Insulin secretion of HIT-T15 treated with (-)-epicatechin.
그림 Fig. 16. Insulin secretion of HIT-T15 treated with taxifolin-3′-O-β-D-(+)-glucose.
$Fig. 11. Insulin secretion of HIT-T15 treated with the EtOAc soluble fraction.$ 그림 Fig. 11. Insulin secretion of HIT-T15 treated with the EtOAc soluble fraction.
그림 Fig. 13. Insulin secretion of HIT-T15 treated with (+)-catechin.
그림 Fig. 15. Insulin secretion of HIT-T15 treated with taxifolin.
그림 Fig. 17. Insulin secretion of HIT-T15 treated with ρ-coumaric acid.
그림 Fig. 18. Effects of crude on the viability of L6 cells.
$Fig. 19. Effects of EtOAc soluble fraction on the viability of L6 cells.$ 그림 Fig. 19. Effects of EtOAc soluble fraction on the viability of L6 cells.
$Fig. 20. Effects of H2O soluble fraction on the viability of L6 cells.$ 그림 Fig. 20. Effects of H₂O soluble fraction on the viability of L6 cells.
그림 Fig. 21. Effects of (+)-catechin on the viability of L6 cells.
그림 Fig. 22. Effects of (-)-epicatechin on the viability of L6 cells.
그림 Fig. 23. Effects of taxifolin on the viability of L6 cells.
그림 Fig. 24. Effects of taxifolin-3′-O-β-D-(+)-glucose on the viability of L6 cells.
그림 Fig. 25. Effects of ρ-coumaric acid on the viability of L6 cells.
그림 Fig. 26. Glucose uptake activities of the crude.
$Fig. 28. Glucose uptake activities of the H2O soluble fraction.$ 그림 Fig. 28. Glucose uptake activities of the H₂O soluble fraction.
그림 Fig. 30. Glucose uptake activities of (-)-epicatechin.
그림 Fig. 32. Glucose uptake activities of taxifolin-3′-O-β-D-(+)-glucose.
$Fig. 27. Glucose uptake activities of the EtOAc soluble fraction.$ 그림 Fig. 27. Glucose uptake activities of the EtOAc soluble fraction.
그림 Fig. 29. Glucose uptake activities of (+)-catechin.
그림 Fig. 31. Glucose uptake activities of taxifolin.
그림 Fig. 33. Glucose uptake activities of ρ-coumaric acid.

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문제 정의

Therefore, this study was carried out to investigate the chemical constituents of the extracts of Korean red pine inner bark and to confirm the potential for functional application by the evaluation of antidiabetic activity of the extracts.

대상 데이터

HIT-T15 hamster pancreatic β-cells were purchased from the Korean Cell Bank (Seoul, Korea).
Korean red pine inner bark (2.11 kg), collected at the experimental forest of Kangwon National University, was air-dried in the laboratory for about two weeks, ground to fine powder, extracted three times with 70 % aqueous ethanol at room temperature, and then concentrated under vacuum pressure. The concentrated crude was sequentially fractionated with n-hexane, chloroform (CHCl₃), ethyl acetate (EtOAc) and H₂O using a separatory funnel (Table 1).

성능/효과

Based on the above results, the crude, EtOAc soluble fraction, H2O soluble fraction, and isolated compounds of Korean red pine extracts did not show any significant results on the insulin secretion of pancreas β-cells except taxifolin, but indicated the significant differences on the glucose uptake activities.

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