[논문]Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development

Hu, Wen-Xing; Kim, Byoung-Jo; Kwak, Younghwan; Seo, Eun-Young; Kim, Jung-Kyu; Han, Jae-Yeong; Kim, Ik-Hyun; Lim, Yong Pyo; Cho, In-Sook; Domier, Leslie L; Hammond, John; Lim, Hyoun-Sub

doi:10.5423/ppj.nt.11.2018.0238

Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development 원문보기

The plant pathology journal, v.35 no.4, 2019년, pp.381 - 388

Hu, Wen-Xing (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Kim, Byoung-Jo (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Kwak, Younghwan (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Kim, Jung-Kyu (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Han, Jae-Yeong (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Kim, Ik-Hyun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) , Lim, Yong Pyo (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University) , Cho, In-Sook (National Institute of Horticultural & Herbal Science, Rural Development Administration) , Domier, Leslie L (Soybean) , Hammond, John , Lim, Hyoun-Sub

Abstract ▼ AI-Helper

For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey on Jeju Island in 2017. Full-length cDNAs of each isolate were cloned into the pJY vector downstream of cauliflower mosaic virus 35S and bacteriophage T7 RNA polymerase promoters. Their fulllength sequences share 98.9-99.9% nucleotide sequence identity and were most closely related to previously reported Korean TuMV isolates. All isolates belonged to the BR group and infected both Chinese cabbage and radish. Four isolates induced very mild symptoms in Nicotiana benthamiana but KBJ5 induced a hypersensitive response. Symptom differences may result from three amino acid differences uniquely present in KBJ5; Gly(382)Asp, Ile(891)Val, and Lys(2522)Glu in P1, P3, and NIb, respectively.

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그림 Fig. 1. Symptoms of the five isolates (KBJ1-KBJ5) inoculated to Nicotiana benthamiana, shown at 7 days post inoculation (dpi) and 21 dpi; and to radish (Raphanus sativus) cultivars Chunghua and Iljin at 14 dpi. Representative plants inoculated with each isolate are shown. TuMV infection of all radish plants was confirmed by RT-PCR.
그림 Fig. 2. Amino acid residues differing among isolates KBJ1-5. Isolate KBJ-5 is marked in red because it can induce systemic necrosis in Nicotiana benthamiana. Differing amino acid residues are shown in red or blue, with unique KBJ5 residues 382 (HC-Pro), 891 (P3) and 2522 (NIb) marked by a red box.
그림 Fig. 3. Phylogenetic tree constructed by the maximum-likelihood method with 1,000 bootstrap replicates, based on the complete genome sequences of 58 TuMV isolates including twenty other Korean isolates (Gong et al., 2019) and the five new isolates from Jeju (indicated by arrows to the right of the isolate/accession number/country label).
그림 Fig. 4. (A) Structure of the chimeras constructed between mild isolate KBJ4 and severe isolate KBJ5. Chimeras AAB and BBA were generated by exchange of a 5ʹ ApaI/SpeI fragment; to create chimeras ABB and ABA, mutagenic PCR was utilized to alter a single nt resulting in I891V in the ApaI/SpeI fragment. (B) Each chimeric construct was then used to infect Nicotiana benthamiana, and radish cultivars Chunghua and Iljin. Images of representative plants were captured at 24 days post inoculation (dpi) for N. benthamiana, and 14 dpi for radish.

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Additional TuMV sequences were obtained from NCBI Genbank. Phylogenetic relationship of TuMV isolates in this work were analyzed together with twenty previously reported Korean TuMV isolates (Gong et al., 2019; Han et al., 2016) and 33 previously characterized TuMV isolates (Nguyen et al., 2013a) from NCBI. All isolates were compared based on aa and nt sequences.

참고문헌 (31)

10.1016/b978-0-12-384684-6.00093-8
정진수, 한재영, 김정규, 주혜경, 공준수, 서은영, , 임현섭. 2014년 전국 무 재배지의 바이러스 병 발생 조사. Research in plant disease = 식물병연구, vol.21, no.3, 235-242.

원문보기 상세보기
Fauquet 1162 2005
Gal-On, Amit. A Point Mutation in the FRNK Motif of the Potyvirus Helper Component-Protease Gene Alters Symptom Expression in Cucurbits and Elicits Protection Against the Severe Homologous Virus. Phytopathology, vol.90, no.5, 467-473.

상세보기
Gong, Junsu, Ju, Hye-Kyoung, Kim, Ik-Hyun, Seo, Eun-Young, Cho, In-Sook, Hu, Wen-Xing, Han, Jae-Yeong, Kim, Jung-Kyu, Choi, Su Ryun, Lim, Young Pyo, Hammond, John, Lim, Hyoun-Sub. Sequence Variations Among 17 New Radish Isolates of Turnip mosaic virus Showing Differential Pathogenicity and Infectivity in Nicotiana benthamiana, Brassica rapa, and Raphanus sativus. Phytopathology, vol.109, no.5, 904-912.

상세보기
Hall, Barry G.. Building Phylogenetic Trees from Molecular Data with MEGA. Molecular biology and evolution, vol.30, no.5, 1229-1235.

상세보기
Recent occurrence of TuMV disease on radish and Chinese cabbage in alpine region, Kangwon province Hahm 45 1995
Surveys on disease occurrence in major horticultural crops in Kangwon alpine areas Hahm 668 1998
Han, Jae-Yeong, Chung, Jinsoo, Kim, Jungkyu, Seo, Eun-Young, Kilcrease, James P., Bauchan, Gary R., Lim, Seungmo, Hammond, John, Lim, Hyoun-Sub. Comparison of helper component-protease RNA silencing suppression activity, subcellular localization, and aggregation of three Korean isolates of Turnip mosaic virus. Virus genes, vol.52, no.4, 592-596.

상세보기
Jenner, Carol E., Tomimura, Kenta, Ohshima, Kazusato, Hughes, Sara L., Walsh, John A.. Mutations in Turnip mosaic virus P3 and Cylindrical Inclusion Proteins Are Separately Required to Overcome Two Brassica napus Resistance Genes. Virology, vol.300, no.1, 50-59.

상세보기
Jenner, Carol E., Wang, Xiaowu, Tomimura, Kenta, Ohshima, Kazusato, Ponz, Fernando, Walsh, John A.. The Dual Role of the Potyvirus P3 Protein ofTurnip mosaic virusas a Symptom and Avirulence Determinant in Brassicas. Molecular plant-microbe interactions : MPMI, vol.16, no.9, 777-784.

상세보기
김정수, 이수헌, 최홍수, 김미경, 곽해련, 김정선, 남문, 조점덕, 조인숙, 최국선. 2007-2011 우리나라 농업현장 임상진단 요청 작물의 바이러스 감염 특성. Research in plant disease = 식물병연구, vol.18, no.4, 277-289.

원문보기 상세보기
A Turnip mosaic virus determinant of systemic necrosis in Nicotiana benthamiana , and a novel resistance-breaking determinant in Chinese cabbage identified from chimeric infectious clones Kim 2019 10.1094/PHYTO-08-18-0323-R

상세보기
Lim, Hyoun-Sub, Bragg, Jennifer N., Ganesan, Uma, Ruzin, Steven, Schichnes, Denise, Lee, Mi Yeon, Vaira, Anna Maria, Ryu, Ki Hyun, Hammond, John, Jackson, Andrew O.. Subcellular Localization of the Barley Stripe Mosaic Virus Triple Gene Block Proteins. Journal of virology, vol.83, no.18, 9432-9448.

상세보기
Li, Mei-Jia, Kim, Jung-Kyu, Seo, Eun-Young, Hong, Seok Myeong, Hwang, Eui-Il, Moon, Jung-Kyung, Domier, Leslie L., Hammond, John, Youn, Young-Nam, Lim, Hyoun-Sub. Sequence variability in the HC-Pro coding regions of Korean soybean mosaic virus isolates is associated with differences in RNA silencing suppression. Archives of virology, vol.159, no.6, 1373-1383.

상세보기
McKeown, A.W., Warland, J., McDonald, M.R., Hutchinson, C.M.. COOL SEASON CROP PRODUCTION TRENDS: A POSSIBLE SIGNAL FOR GLOBAL WARMING. Acta horticulturae, vol.638, 241-248.

상세보기
Nguyen, H.D., Tran, H.T.N., Ohshima, K.. Genetic variation of the Turnip mosaic virus population of Vietnam: A case study of founder, regional and local influences. Virus research : an international journal of molecular and cellular virology, vol.171, no.1, 138-149.

상세보기
Nguyen, Huy D., Tomitaka, Yasuhiro, Ho, Simon Y. W., Duchêne, Sebastián, Vetten, Heinrich-Josef, Lesemann, Dietrich, Walsh, John A., Gibbs, Adrian J., Ohshima, Kazusato. Turnip Mosaic Potyvirus Probably First Spread to Eurasian Brassica Crops from Wild Orchids about 1000 Years Ago. PloS one, vol.8, no.2, e55336-.

상세보기
Ohshima, Kazusato, Yamaguchi, Yuka, Hirota, Ryo, Hamamoto, Tamaki, Tomimura, Kenta, Tan, Zhongyang, Sano, Teruo, Azuhata, Fumio, Walsh, John A., Fletcher, John, Chen, Jishuang, Gera, Abed, Gibbs, Adrian. Molecular evolution of Turnip mosaic virus: evidence of host adaptation, genetic recombination and geographical spread. The Journal of general virology, vol.83, no.6, 1511-1521.

상세보기
Oh, Soonja, Moon, Kyung Hwan, Song, Eun Young, Son, In-Chang, Koh, Seok Chan. Photosynthesis of Chinese cabbage and radish in response to rising leaf temperature during spring. Horticulture, Environment, and Biotechnology, vol.56, no.2, 159-166.

상세보기
Park, Chan-Hwan, Ju, Hye-Kyoung, Han, Jae-Yeong, Park, Jong-Seo, Kim, Ik-Hyun, Seo, Eun-Young, Kim, Jung-Kyu, Hammond, John, Lim, Hyoun-Sub. Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters. Virus genes, vol.53, no.2, 286-299.

상세보기
Turnip mosaic potyvirus Provvidenti 1340 1996
Redondo, Elise, Krause-Sakate, Renate, Yang, Shu-Jun, Lot, Hervé, Le Gall, Olivier, Candresse, Thierry. Lettuce mosaic virus Pathogenicity Determinants in Susceptible and Tolerant Lettuce Cultivars Map to Different Regions of the Viral Genome. Molecular plant-microbe interactions : MPMI, vol.14, no.6, 804-810.

상세보기
Riechmann, José Luis, Dallot, Silvie, Quiot, Laurence, Garcı́a, Juan Antonio, Sáenz, Pilar, Cervera, Maria Teresa, Quiot, Jean-Bernard. Identification of a pathogenicity determinant of Plum pox virus in the sequence encoding the C-terminal region of protein P3+6K1. The Journal of general virology, vol.81, no.3, 557-566.

상세보기
Sáenz, Pilar, Quiot, Laurence, Quiot, Jean-Bernard, Candresse, Thierry, García, Juan Antonio. Pathogenicity Determinants in the Complex Virus Population of aPlum pox virusIsolate. Molecular plant-microbe interactions : MPMI, vol.14, no.3, 278-287.

상세보기
Suehiro, Noriko, Natsuaki, Tomohide, Watanabe, Tomoko, Okuda, Seiichi. An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein. The Journal of general virology, vol.85, no.7, 2087-2098.

상세보기
Tomimura, K., Gibbs, A. J., Jenner, C. E., Walsh, J. A., Ohshima, K.. The phylogeny of Turnip mosaic virus; comparisons of 38 genomic sequences reveal a Eurasian origin and a recent ‘emergence’ in east Asia. Molecular ecology, vol.12, no.8, 2099-2111.

상세보기
Tomimura, Kenta, Špak, Josef, Katis, Nikos, Jenner, Carol E., Walsh, John A., Gibbs, Adrian J., Ohshima, Kazusato. Comparisons of the genetic structure of populations of Turnip mosaic virus in West and East Eurasia. Virology, vol.330, no.2, 408-423.

상세보기
TOMITAKA, YASUHIRO, OHSHIMA, KAZUSATO. A phylogeographical study of the Turnip mosaic virus population in East Asia reveals an ‘emergent’ lineage in Japan. Molecular ecology, vol.15, no.14, 4437-4457.

상세보기
Yasaka, Ryosuke, Fukagawa, Hirofumi, Ikematsu, Mutsumi, Soda, Hiroko, Korkmaz, Savas, Golnaraghi, Alireza, Katis, Nikolaos, Ho, Simon Y. W., Gibbs, Adrian J., Ohshima, Kazusato. The Timescale of Emergence and Spread of Turnip Mosaic Potyvirus. Scientific reports, vol.7, 4240-.

상세보기
Research progress and perspective of tripartite interaction of virus-vector-plant in vector-borne viral diseases Ye 845 2017

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