[논문]비만세포 매개 즉시형 과민반응에 대한 표고버섯 추출물의 보호 효과

연광해; 최윤호

비만세포 매개 즉시형 과민반응에 대한 표고버섯 추출물의 보호 효과
The Protective Effect of Lentinus Edodes on Mast Cell-Mediated Immediate-Type Hypersensitivity 원문보기

생약학회지, v.50 no.3, 2019년, pp.175 - 184

연광해 (중국 연변대학교 해부조직발생학교실) , 최윤호 (전북대학교 의과대학 해부학교실)

Abstract ▼ AI-Helper

Mast cells are crucial as effector cells in the immediate-type allergic reaction. Lentinus edodes has been the popular edible mushroom in oriental countries and reported to have immunomodulatory, anti-tumor, anti-atherogenic, anti-viral, and anti-allergic activities. However, the roles of L. edodes in mast cell-mediated anaphylactic reaction have not been fully elucidated. In this research, we have demonstrated the effects of the methanol extract of L. edodes (MELE) on mast cell-mediated anaphylaxis-like and anaphylactic reactions. MELE suppressed systemic anaphylaxis-like reaction, plasma histamine levels, and ear swelling response in mice treated with compound 48/80. MELE also suppressed passive systemic and cutaneous anaphylaxis mediated by anti-dinitrophenyl IgE. In accordance with these findings, MELE dose-dependently decreased histamine release from RPMC evoked by compound 48/80 or the antigen-antibody reaction. To clarify the mechanism of degranulation system, intracellular cAMP levels as well as calcium influx in RPMC was evaluated. In compound 48/80-treated RPMC, MELE blocked calcium uptake into the cells. In addition, MELE elevated the intracellular cAMP content and significantly attenuated compound 48/80-induced cAMP reduction in RPMC. Taken together, we propose the clinical use of MELE in mast cell-mediated immediate-type allergic diseases.

주제어

표/그림 (9)

표 Table I. Inhibitory effect of the methanol extract of L. edodes (MELE) on compound 48/80-stimulated systemic anaphylaxis-like reaction in mice
그림 Fig. 1. Effect of the methanol extract of L. edodes (MELE) on compound 48/80-stimulated plasma histamine levels in mice.
그림 Fig. 2. Effect of the methanol extract of L. edodes (MELE) on anti-DNP IgE-mediated passive systemic anaphylaxis in mice.
표 Table II. Inhibitory effect of the methanol extract of L. edodes (MELE) on compound 48/80-stimulated ear edema in mice
그림 Fig. 3. Effect of the methanol extract of L. edodes (MELE) on anti-DNP IgE-mediated passive cutaneous anaphylaxis in rats.
그림 Fig. 4. Inhibitory effect of the methanol extract of L. edodes (MELE) on compound 48/80-stimulated histamine release from rat peritoneal mast cells (RPMC).
그림 Fig. 5. Inhibitory effect of the methanol extract of L. edodes (MELE) on anti-DNP IgE-mediated histamine release from rat peritoneal mast cells (RPMC).
그림 Fig. 6. Inhibitory effect of the methanol extract of L. edodes (MELE) on compound 48/80-stimulated calcium uptake in rat peritoneal mast cells (RPMC).
그림 Fig. 7. Inhibitory effect of the methanol extract of L. edodes (MELE) on compound 48/80-stimulated decrease of cAMP level in rat peritoneal mast cells (RPMC).

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제안 방법

To elucidate the effect of MELE on IgE-mediated mast cell activation, RPMC were also treated with anti-DNP IgE (10 μg/mL) for 6 h and preincubated with MELE or DSCG at 37℃ for 10 min before antigenic challenge with DNP-HSA (100 ng/mL).

대상 데이터

edodes used in this study were purchased from Jangsu Oriental Pharmacy (Jeonbuk, Korea). A voucher specimen (number 2011- LEMw705025) was deposited in its herbarium of Research Center, Medical School, Chonbuk National University. The air-dried fruiting bodies (120 g) were immersed in 600 mL of 70% methanol, followed by keeping overnight in a refrigerator (4℃), and boiled under reflux for 2 h.
Preparation of the Methanol Extract of L. edodes (MELE) − The dried fruiting bodies of L. edodes used in this study were purchased from Jangsu Oriental Pharmacy (Jeonbuk, Korea).

이론/모형

Effect of MELE on Mast Cell Viability − MTT colorimetric test was used to quantify the viability of RPMC exposed to MELE.
5 min later, mice were killed by cervical dislocation and blood samples were then collected by cardiac puncture. Plasma was isolated from blood samples and plasma histamine concentration was measured by the radioenzymatic method. Oral administration of MELE (0.
The samples were centrifuged at 150×g for 12 min at 4℃, and then the content of histamine in the supernatant was estimated by the radioenzymatic method.

성능/효과

Effect of MELE on anti-DNP IgE-mediated PCA − Similar to ear swelling response evoked by compound 48/ 80, local extravasation could also be elicited by a local allergic reaction between anti-DNP IgE and DNP-HSA. After 48 hours, as revealed by extravasation of Evans blue, the PCA reaction was significantly reduced by 0.01 to 1 g/ kg BW of MELE (Fig. 3).
Our results obtained in this study have proved that MELE inhibits both compound 48/80-stimulated anaphylaxis-like reaction and IgE-mediated anaphylactic reaction in vivo and in vitro in a murine mast cell model. However, some previous reports have introduced that the consumption of raw or undercooked L.
The results of this study demonstrate that MELE has anti-anaphylactic and anti-allergic properties. The murine models of anaphylaxis-like reaction were developed to assess the contribution of MELE in mast cell-dependent anaphylactoid reactions.
MELE dose-dependently suppressed IgE-mediated histamine secretion from RPMC. These results suggest that MELE contains the active compounds, which mitigate compound 48/80-induced or IgE-mediated anaphylaxis-like responses by blocking histamine secretion from RPMC.

후속연구

Given this, the additional effort to identify active ingredients from MELE should be performed in subsequent work. Furthermore, future studies will be required to elucidate the possibility that MELE may also be effective in the human mast cells and in the treatment of human allergic disorders such as asthma and allergic rhinitis. In conclusions, our present data indicate that L.
Future study will be necessary to elucidate the detailed mechanism of MELE inhibition in FcεRI-induced degranulation of mast cells.

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