Kim, Tae-rahk
(Center for Research and Development, LACTOMASON)
,
Choi, Kyoung-sook
(Center for Research and Development, LACTOMASON)
,
Ji, Yosep
(Advanced Green Energy and Environment, Handong Global University)
,
Holzapfel, Wilhelm H.
(Advanced Green Energy and Environment, Handong Global University)
,
Jeon, Min-Gyu
(Center for Research and Development, LACTOMASON)
Lactic acid bacteria are well-known probiotics, conferring several health benefits. In this study, we isolated lactobacilli from human breast milk and identified Lactobacillus reuteri LM1071 (RR-LM1071) using 16S rDNA sequencing. We tested the hemolytic activity, biogenic amine production, and antib...
Lactic acid bacteria are well-known probiotics, conferring several health benefits. In this study, we isolated lactobacilli from human breast milk and identified Lactobacillus reuteri LM1071 (RR-LM1071) using 16S rDNA sequencing. We tested the hemolytic activity, biogenic amine production, and antibiotic susceptibility of this strain to assess its safety. RR-LM1071 was found to be negative for hemolytic activity and biogenic amine production, as well as was measured in susceptible level for antibiotics in the minimal inhibitory concentration (MIC) test. The adhesive properties of RR-LM1071 were higher than those of LGG in HT-29 cells, and showed a greater hydrophobicity than LGG in hexadecane solvent. Under inflammatory conditions, RR-LM1071 suppressed the mRNA expression of IL-6, TNF-α, and IL-4 produced in IL-1β-induced HT-29 cells. Our results suggest that RR-LM1071 is a safe and valuable probiotic that can be used for the treatment of inflammatory bowel disease.
Lactic acid bacteria are well-known probiotics, conferring several health benefits. In this study, we isolated lactobacilli from human breast milk and identified Lactobacillus reuteri LM1071 (RR-LM1071) using 16S rDNA sequencing. We tested the hemolytic activity, biogenic amine production, and antibiotic susceptibility of this strain to assess its safety. RR-LM1071 was found to be negative for hemolytic activity and biogenic amine production, as well as was measured in susceptible level for antibiotics in the minimal inhibitory concentration (MIC) test. The adhesive properties of RR-LM1071 were higher than those of LGG in HT-29 cells, and showed a greater hydrophobicity than LGG in hexadecane solvent. Under inflammatory conditions, RR-LM1071 suppressed the mRNA expression of IL-6, TNF-α, and IL-4 produced in IL-1β-induced HT-29 cells. Our results suggest that RR-LM1071 is a safe and valuable probiotic that can be used for the treatment of inflammatory bowel disease.
In this study, we evaluated the potential risk of RR-LM1071 isolated from breast milk by testing its hemolytic activity, biogenic amine production, and antibiotic resistance. To propose RRLM1071 as an effective probiotic for regulating intestinal inflammation, we tested its adhesive properties and anti-inflammatory effects on HT-29 cells.
데이터처리
Mean differences were evaluated by Student’s t-test or one-way ANOVA followed by tukey’s multiple comparison test
성능/효과
Under inflammatory conditions induced by IL-1β in HT-29 cells, RR-LM1071 suppressed the expression of inflammatory cytokines, namely IL-6, TNF-α, and IL-4, via the MAP kinase pathway. Collectively, our results demonstrate that RR-LM1071 would be an effective and safe probiotic in humans, thus making the case for its use in the development of health supplements and therapeutic treatments for IBD.
In this study, the safety of RR-LM 1071 isolated from human breast milk was demonstrated by evaluating its hemolytic activity, biogenic amine production, and antibiotic resistance. In addition, RR-LM1071 was found to have more affinity for HT-29 cells compared to LGG in adhesive tests. Under inflammatory conditions induced by IL-1β in HT-29 cells, RR-LM1071 suppressed the expression of inflammatory cytokines, namely IL-6, TNF-α, and IL-4, via the MAP kinase pathway.
In this study, the safety of RR-LM 1071 isolated from human breast milk was demonstrated by evaluating its hemolytic activity, biogenic amine production, and antibiotic resistance. In addition, RR-LM1071 was found to have more affinity for HT-29 cells compared to LGG in adhesive tests.
Under inflammatory conditions induced by IL-1β in HT-29 cells, RR-LM1071 suppressed the expression of inflammatory cytokines, namely IL-6, TNF-α, and IL-4, via the MAP kinase pathway
후속연구
Although the findings presented in this study point to the involvement of MAP kinase activation, which is responsible for the activation of inflammatory genes, future studies will need to confirm whether RR-LM1071 specifically regulates the pathway in HT-29 cells, as well as evaluate whether other pathways, such as the NF-κB pathway mediated by IL-1β, are involved.
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