Jung, Myung-A
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Shin, Jawon
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Jo, Ara
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Kang, Huwon
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Lee, Gyuok
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Oh, Dool-Ri
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Yun, Hyo Jeong
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Im, Sojeong
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Bae, Donghyuck
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Kim, Jaeyong
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
,
Choi, Chul-yung
(Jeonnam Bioindustry Foundation, Center of Natural Resources Research (JCNR))
Testosterone deficiency syndrome (TDS), also known as late-onset hypogonadism, is a clinical and biochemical syndrome associated with advanced age and characterized by deficient serum testosterone levels. The Elaeagnus multiflora fruit (EMF) and Cynanchum wilfordii (CW) have been used in traditional...
Testosterone deficiency syndrome (TDS), also known as late-onset hypogonadism, is a clinical and biochemical syndrome associated with advanced age and characterized by deficient serum testosterone levels. The Elaeagnus multiflora fruit (EMF) and Cynanchum wilfordii (CW) have been used in traditional herbal medicine. This study aimed to investigate the therapeutic effects of EMF and CW mixtures (at the ratios of 3:7, 5:5, and 7:3) on TDS using TM3 cells and aging male rats. EMF, and mixtures of EMF and CW (at the ratios of 3:7, 5:5, and 7:3) significantly increased testosterone levels in TM3 cells (p <0.05). The rats were orally administered EMCW (EMF and CW mixed at the ratio of 3:7 50, 100 and 200 mg/kg/day) for 4 weeks consecutively. After 4 weeks of EMCW administration, latency time on the rotarod test, and serum testosterone and dehydroepiandrosterone sulfate levels were significantly increased (p <0.05 and p <0.01). Moreover, the levels of globulin-bound sex hormones were decreased in the EMCW-fed groups. However, prostate-specific antigen levels did not differ among the groups. These results suggest that EMCW can be effectively used to alleviate TDS.
Testosterone deficiency syndrome (TDS), also known as late-onset hypogonadism, is a clinical and biochemical syndrome associated with advanced age and characterized by deficient serum testosterone levels. The Elaeagnus multiflora fruit (EMF) and Cynanchum wilfordii (CW) have been used in traditional herbal medicine. This study aimed to investigate the therapeutic effects of EMF and CW mixtures (at the ratios of 3:7, 5:5, and 7:3) on TDS using TM3 cells and aging male rats. EMF, and mixtures of EMF and CW (at the ratios of 3:7, 5:5, and 7:3) significantly increased testosterone levels in TM3 cells (p <0.05). The rats were orally administered EMCW (EMF and CW mixed at the ratio of 3:7 50, 100 and 200 mg/kg/day) for 4 weeks consecutively. After 4 weeks of EMCW administration, latency time on the rotarod test, and serum testosterone and dehydroepiandrosterone sulfate levels were significantly increased (p <0.05 and p <0.01). Moreover, the levels of globulin-bound sex hormones were decreased in the EMCW-fed groups. However, prostate-specific antigen levels did not differ among the groups. These results suggest that EMCW can be effectively used to alleviate TDS.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
The rats were divided into four groups (n=5), and received the fallowing treatments: Group I, vehicle-treated (distilled water; oral administration [p.o.]); Group Ⅱ, EMCW 50㎎/㎏/day (p, o); Group Ⅲ, EMCW 100㎎/㎏/day (p, o); Group IV; EMCW 200 ㎎/㎏/day (p.o.). EMCW was dissolved in distilled water and administered at 9:30 a.
To evaluate the effects of EMCW on the adaptation of rat skeletal muscles to aging, tests were performed to assess physical strength. We investigated the effects of EMCW on the aging male rats using rotarod tests (Fig.
When testosterone is bound to SHBG, it cannot be used by the body [30]. To investigate the effects of EMCW on serum SHBG levels in aging male rats, EMCW (50, 100, and 200㎎/㎏/day) was administered daily for 4 weeks. Thereafter, SHBG were lower in EMCW than those in the control group (Fig.
seminiferous tubes [27]. Using TM3 cells, we evaluated testosterone production after treatment with EMF, CW, and mixtures of the two extracts in different ratios (Fig. 1). Each compound tested was used at concentrations of 50 or 100µg/mL.
대상 데이터
The EMFs used in this study were obtained from Yeosu-si (Jeollanamdo, Republic of Korea) and authenticated by Dr. Choi at the Jeonnam Bioindustry Foundation, Center of Nature Resources Research (JCNR), Jangheung, Jeollanamdo, Republic of Korea. The plant used for this study was purchased from the Kangwons Medicinal Herb Association (Wonjusi, Kangwondo, Republic of Korea).
Choi at the Jeonnam Bioindustry Foundation, Center of Nature Resources Research (JCNR), Jangheung, Jeollanamdo, Republic of Korea. The plant used for this study was purchased from the Kangwons Medicinal Herb Association (Wonjusi, Kangwondo, Republic of Korea). Air-dried EMF (100 g) was extracted with 20 volumes of water at 100oC for 3h.
, commonly called oriental cherry silverberry or gumi, belongs to the family Elaeagnaceae. The species is native to China, Korea, and Japan. Several parts of E.
데이터처리
, San Diego, CA, USA) software. Differences between groups were assessed using Dunnett’s multiple range tests. Statistical significance was considered at p<0.
성능/효과
2. In all groups, the body weights gradually increased from week 1 until week 3. Furthermore, in all groups, the mean body weight decreased at 4 weeks. However, final body weights of the EMCW (50, 100, and 200㎎/㎏/day)-treated groups were not significantly different from those of the control groups.
참고문헌 (32)
Hanker FD (1977) Sexual, psychic and physical complaints in 50 middle-aged men. Psychosomatics 18(5): 23-27
Harman SM, Metter EJ, Tobin JD (2001) Longitudinal effects of aging on serum total and free testosterone levels in healthy men. Baltimore Longitudinal Study of Aging. J Clin Endocrinol Metab 86(2): 724-731
Morales A, Schulman CC, Tostain JCW, Wu F (2006) Testosterone Deficiency Syndrome (TDS) needs to be named appropriately-the importance of accurate terminology. Eur Urol 50: 407-409
Wang C, Nieschlag E, Swerdloff R, Behre HM, Hellstrom L, Gooren LJ, Kaufman JM, Legross JJ, Lunenfeld B, Morles A, Morley JE, Schulman C, Thompson IM, Weidner W, Wu FCW (2009) ISA, ISSAM, EAU, EAA and ASA recommendations: Investigation, treatment and monitoring of late-onset hypogonadism in males. Int J Impot Res 21: 1-8
Haring R, Baumeister SE, Nauck M, Volzke H, Keevil BG, Brabant G, Wallaschocski H (2013) Testosterone and cardiometabolic risk in the general population the impact of measurement method on risk associations: a comparative study between immunoassay and mass spectrometry. Eur J Endocrinol 169(4): 1-22
Feldman HA, Longcope C, Derby CA (2002) Age trends in the level of serum testosterone and other hormones in middle-aged men: longitudinal results from the Massachusetts male aging study. J Clin Endocrinal Metab 87(2): 589-598
Su C, Rybalchenko N, Schreihofer DA, Singh M, Abbassi B, Cunningham RL (2011) Cell models for the study of sex steroid hormone neurobiology. J Steroid Hormone Sci 2: 1-9
Nieschlag E, Swerdloff R, Behre HM, Gooren LJ, Kaufman JM, Legros J-J (2005) Investigation, treatment and monitoring of late-onset hypogonadism in males. Int J Androl 8: 56-58
Sakamura F, Suga T (1987) Changes in chemical components of ripening oleaster fruits. Phytochemistry 26(9): 2481-2484
Kim JG (2006) Illustrated natural drugs encyclopedia. Namsandong, Seoul, Korea, p 279
Yoon KY, Hong JY, Nam HS, Moon YS, Shin SR (2007) Antioxidant activities and xanthine oxidase inhibitory effects of hot-water extracts from fruits of Elaeagnus multiflora Thunb. in maturity. J Korean Soc Food Sci Nutr 36(1): 14-19
Hong JY, Nam HS, Lee YS, Yoon KY, Kim NW, Shin SR (2006) Study on the antioxidant activity of extracts from the fruit of Elaeagnus multiflora Thunb. Korea J Food Preserv 13(3): 413-419
Kim SA, Oh SI, Lee MS (2007) Antioxidative and cytotoxic effects of solvent fractions from Elaeagnus multiflora. Korean J Food Nutr 20(2): 134-142
Kim NW, Joo EY, Kim SL (2003) Analysis on the components of the fruit of Elaeagnus multiflora Thunb. Korean J Food Preserv 10: 534-539
Hong JY, Nam HS, Lee YS, Kim NW, Shin SR (2006) Anti-oxidant activity of ethanol extracts from fruits of Elaeagnus multiflora Thunb. during maturation. Korean J Food Preserv 13(5): 643-648
Shin SR, Hong JY, Yoon KY (2008) Antioxidant properties and total phenolic contents of cherry elaeagnus (Elaeagnus multiflora Thunb.) leaf extract. Food Sci Biotechnol 17(3): 608-612
Lachowicz S, Kapusta I, Swieca M, Stinco CM, Melendez-Martinez AJ, Bieniek A (2020) In vitro biological activities of fruits and leaves of Elaeagnus multiflora Thunb. and their isoprenoids and polyphenolics profile. Antioxidants 9(5): 1-17
Jung MA, Jo AR, Shin JW, Kang HW, Kim YJ, Oh DR, Choi CY (2020) Anti-fatigue effects of Elaeagnus multiflora fruit extracts in mice. J Appl Biol Chem 63(1): 69-74
Choi DH, Lee YJ, Kim J S, Kang DG, Lee HS (2012) Cynanchum wilfordii ameliorates hypertension and endothelial dysfunction in rats fed with high fat/cholesterol diets. Immunopharmacol Immunotoxicol 34(1): 4-11
Lee HS, Choi JH, Kim YE, Kim IH, Kim BM, Lee CH (2013) Effects of the Cynanchum wilfordii ethanol extract on the serum lipid profile in hypercholesterolemic rats. Prev Nutr Food Sci 18(3): 157-162
Lee HS, Kim MH, Choi YY, Hong JK, Yang WM (2018) Effects of Cynanchum wilfordii on osteoporosis with inhibition of bone resorption and induction of bone formation. Mol Med Rep 17(3): 3758-3762
Shan L, Liu RH, Shen YH, Zang WD, Zhang C, Wu DZ, Min L, Su J, Xu XK (2006) Gastroprotective effect of a traditional Chinese herbal drug 'Bioshouwu on experimental gastric lesions in rat. J Ethnopharmacol 107(3): 389-394
Kim MS, Baek JH, Park JA, Hwang BY, Kim SE, Lee JJ, Kim KW (2005) Wilfoside KIN isolated from Cynanchum wilfordii inhibits angiogenesis and tumor cell invasion. Int J Oncol 26(6): 1533-1539
Yang SB, Lee SM, Park JH, Lee TH, Baek NI, Park HJ, Lee HJ, Kim JY (2014) Cynandion A from Cynanchum wilfordii attenuates the production of inflammatory mediators in LPS-induced BV-2 microglial cells via NF-κB inactivation. Biol Pharm Bull 37(8): 1390-1396
Lee GO, Kim JY, Pan SO, Kim M, Jun WJ, Choi CY (2016) Ahprodisiac property of the aqueous extract of Cynanchum wilfordii. J Food Nutr 4: 713-719
Lee GO, Shin JW, Choi HJ, Jo AR, Pan SO, Bae DH, Lee YW, Choi CY (2017) Cynanchum wilfordii ameliorates testosterone-induced benign prostatic hyperplasia by regulation 5a-reductase and androgen receptor activities in a rat model. Nutrients 9(10): 1-15
Mather JB (1980) Establishment and characterization of two distinct mouse testicular epithelial cell lines. Bio Reprod 23(1): 243-252
Sattler F, Bhasin S, He J, Chou CP, Castaneda-Sceppa C, Yarasheski K, Binder E, Schroeder ET, Kawakubo M, Zhang A, Roubenoff R, Azen S (2011) Testosterone threshold levels and lean tissue mass targets needed to enhance skeletal muscle strength and function: the HORMA trial. J Gerontol A Biol Sci Med Sci 66(1): 122-129
Noh YH, Kim SH, Kim JY, Park JA, Kim OH, Han DS, Kim WY, Kim SS, Lee MY, Heo SH, Kim MS, Lee WB, Jeong YH, Myung SC (2012) Improvement of andropause symptoms by dandelion and rooibos extract complex CRS-10 in aging male. Nutr Res Pract 6(6): 505-512
Arlt W, Haas J, Callies F, Reincke M, Hubler D, Oettel M, Ernst M, Schulte HM, Allolio B (1999) Biotransformation of oral dehydroepiandrosterone in elderly men: significant increase in circulating estrogens. J Clin Endocrinol Metab 84(6): 2170-2176
※ AI-Helper는 부적절한 답변을 할 수 있습니다.