[논문]제비집 시알산 유래 영양전달체(Nutrient Delivery System)의 인지기능 및 운동기능 개선 효과

김동명; 정주영; 이형곤; 권용성; 백진홍; 한인석

doi:10.9799/ksfan.2020.33.6.614

[국내논문] 제비집 시알산 유래 영양전달체(Nutrient Delivery System)의 인지기능 및 운동기능 개선 효과
Improvements in Cognitive and Motor Function by a Nutrient Delivery System Containing Sialic Acid from Edible Bird's Nest 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.33 no.6, 2020년, pp.614 - 623

김동명 ((주)케이제이엠바이오 바이오연구소 총괄연구소) , 정주영 ((주)케이제이엠바이오 바이오연구소) , 이형곤 ((주)케이제이엠바이오 바이오연구소) , 권용성 ((주)케이제이엠바이오 바이오연구소) , 백진홍 ((주)김정문알로에 생명과학연구소) , 한인석 (유타대학교 화학공학과)

Abstract ▼ AI-Helper

The objective of this study was to produce a nutrient delivery system (NDS) using sialic acid extracted from edible bird's nest (EBN), which improves brain function in patients with Alzheimer's disease and Parkinson's disease, by affinity bead technology (ABT). The inhibitory activity of acetylcholinesterase (AChE) and pyramidal cells in the dentate gyrus of the hippocampus were analyzed to investigate the effect of a sialic acid NDS on Alzheimer's disease. Also, the effect of a sialic acid NDS on Parkinson's disease was evaluated by rota-rod test and pole test in an animal model. Among the groups treated with donepezil, EBN, and sialic acid NDS, the AChE activity was the lowest in the sialic acid NDS-treated group. The results of the hippocampus analysis of the rat model confirmed that the sialic acid NDS inhibited amyloid-beta accumulation depending upon the concentration. Also, the sialic acid NDS group showed more improvement in motor deterioration than the1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced group in both the rota-rod test and pole test. Therefore, the sialic acid NDS had an effect of protecting not only Alzheimer's disease by inhibiting AChE and amyloid-beta accumulation, but Parkinson's disease by preventing neurotoxicity induced by MPTP.

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표/그림 (7)

그림 Fig. 1. Process flow diagram of affinity bead technology (ABT).
그림 Fig. 2. Nutrient delivery system which is consisted of sialic acid-bile acid conjugated micelle nano particles.
그림 Fig. 3. HPLC chromatogram of sialic acid after sepa-ration by the affinity bead technology. 1: N-acetyl-neura-minic acid (Neu5Ac); 2: α-anomer of Neu5Ac (NeuAc-α); 3: N-glycolylneuraminic acid (Neu5Gc); 4: β-anomer of Neu5Ac (NeuAc-β).
그림 Fig. 4. Results of in vivo acetylcholinesterase (AChE) activity test of normal mice (control), treated with donepezil as a positive control, treated with extract of bird's nest and treated with sialic acid. Differences between groups were statistically analyzed by ANOVA with post-hoc by Duncan test. **p<0.05 compared to control group.
그림 Fig. 5. Hematoxylin-eosin staining of pyramidal cell in denate gyrus of rat hippocampus. (A) Normal rat, (B) Treated with amyloid β, (C) Treated with amyloid β and 100 mg/kg of sialic acid, (D) Treated with amyloid β and 200 mg/kg of sialic acid.
그림 Fig. 6. Protective effect of sialic acid on 1-methyl-1,2,3,6- tetrahydropyridine (MPTP)-induced behavioral impairment in a mouse Parkinson's disease model using the rota-rod test. (A) Latency to fall, (B) Total distance; Mice in normal group were not treated with MPTP. Mice in MPTP group were treated with 20 mg/kg of MPTP for 5 days. Mice in sialic acid group were treated with 200 mg/kg of sialic acid for 28 days before treating 20 mg/kg of MPTP for 5 days. Differences between groups were statistically analyzed by ANOVA with post-hoc by Duncan test (**p<0.05).
그림 Fig. 7. Protective effect of sialic acid on 1-methyl-1,2,3,6-tetrahydropyridine (MPTP)-induced behavioral impairment in a mouse Parkinson's disease model using the pole test. Mice in normal group were not treated with MPTP. Mice in MPTP group were treated with 20 mg/kg of MPTP for 5 days. Mice in sialic acid group were treated with 200 mg/kg of sialic acid for 28 days before treating 20 mg/kg of MPTP for 5 days. Differences between groups were statistically analyzed by ANOVA with post-hoc by Duncan test (**p<0.05).

참고문헌 (42)

Canal N, Imbimbo BP, Group ES. 1996. Relationship between pharmacodynamic activity and cognitive effects of eptastigmine in patients with Alzheimer's disease. Clin Pharmacol Ther 60:218-228

상세보기
Careena S, Sani D, Tan S, Lim CW, Hassan S, Norhafizah M, Kirby BP, Ideris A, Stanslas J, Bin Basri H. 2018. Effect of edible bird's nest extract on lipopolysaccharide-induced impairment of learning and memory in wistar rats. Evid Based Complementary Altern Med 2018:9318789
Chantler P, Driessens G. 2010. Swifts: A Guide to the Swifts and Treeswifts of the World. 2 nd ed. pp.150-152. Bloomsbury
Chiavegatto S, Sun J, Nelson RJ, Schnaar RL. 2000. A functional role for complex gangliosides: Motor deficits in GM2/GD2 synthase knockout mice. Exp Neurol 166:227-234

상세보기
Colombo JP, Garcia-Rodenas C, Guesry PR, Rey J. 2003. Potential effects of supplementation with amino acids, choline or sialic acid on cognitive development in young infants. Acta Paediatr 92:42-46
Ferger B, Teismann P, Earl CD, Kuschinsky K, Oertel WH. 2000. The protective effects of PBN against MPTP toxicity are independent of hydroxyl radical trapping. Pharmacol Biochem Behav 65:425-431

상세보기
Gandy S. 2011. Perspective: Prevention is better than cure. Nature 475:S15

상세보기
Goh DLM, Chua KY, Chew FT, Liang RCMY, Seow TK, Ou KL, Yi FC, Lee BW. 2001. Immunochemical characterization of edible bird's nest allergens. J Allergy Clin Immunol 107: 1082-1088

상세보기
Halliwell B. 2006. Oxidative stress and neurodegeneration: Where are we now? J Neurochem 97:1634-1658

상세보기
Hidalgo A, Burgos V, Viola H, Medina J, Argibay P. 2006. Differential expression of glycans in the hippocampus of rats trained on an inhibitory learning paradigm. Neuropathology 26:501-507

상세보기
Jeong YS, Hwang BS, Cho SM, Hwang KA, Hwang IG. 2017. Antioxidant and anti-diabetic, anti-Alzheimer activities of stem from Opuntia ficus-indica var. saboten cultivated in Jeju at harvest time. Korean J Food Nutr 30:1332-1340
Kang BG, Seo YM, Lee JW, Kim NM, Hong SK, Baek SS. 2013. Method for preparing antler extract containing increased sialic acid content. Korea Patent 10-2013-0087670
Kang JS. 2016. Effect of medicinal herb composites on antioxidative and cognition-enhancing activities in rats. Korean J Food Nutr 29:382-391
Kasa P, Papp H, Kasa Jr P, Torok I. 2000. Donepezil dosedependently inhibits acetylcholinesterase activity in various areas and in the presynaptic cholinergic and the postsynaptic cholinoceptive enzyme-positive structures in the human and rat brain. Neuroscience 101:89-100
Kathan RH, Weeks DI. 1969. Structure studies of collocalia mucoid: I. Carbohydrate and amino acid composition. Arch Biochem Biophys 134:572-576
Kim DM, Lee HK, Kwon YS. 2020. Purification method of sialic acid in Swallow's nest using ABT. Korea Patent 10-2020-0012156
Kim DM, Cho GS. 2006. Nanofood and its materials as nutrient delivery system (NDS). J Appl Biol Chem 49:39-47
Kim SH, Choi JW. 2013. Antioxidant activity of water extract of Chrysanthemum boreale against MPTP-induced mice models. J Physiol Pathol Korean Med 27:49-56
Kim SH, Han SH. 2012. Prevalence of dementia among the South Korean population. J Korean Diabetes 13:124-128
Korea National Statistical Office. 2018. Polulation census: 2018. Available from http://kostat.go.kr/ [cited 1 September 2020]
Korea National Statistical Office. 2019a. Population projections for Korea: 2017-2067. Available from http://kostat.go.kr/[cited 1 September 2020]
Korea National Statistical Office. 2019b. Long term care insurance statistics. Available from http://kostat.go.kr/ [cited 1 September 2020]
Lee DS, Kim DS, Kim SH. 2013. Herbal compositions for treating neurological diseases and improving memory impairment. Korea Patent 10-2013-0130069
Lee MR, Sun BS, Gu LJ, Wang CY, Fang ZM, Wang Z, Mo EK, Ly SY, Sung CK. 2009. Effects of the deer antler extract on scopolamine-induced memory impairment and its related enzyme activities. J Korean Soc Food Sci Nutr 38: 409-414
Manish G, Vimukta S. 2011. Targeted drug delivery system: A review. Res J Chem Sci 1:135-138
Marni S, Marzura MR, Norzela AM, Khairunnisak M, Bing CH, Eddy AA. 2014. Preliminary study on free sialic acid content of edible bird nest from johor and kelantan. Malays J Vet Res 5:9-14
McClements DJ. 2015. Nanoscale nutrient delivery systems for food applications: Improving bioactive dispersibility, stability, and bioavailability. J Food Sci 80:N1602-N1611

상세보기
McLaurin J, Franklin T, Fraser PE, Chakrabartty A. 1998. Structural transitions associated with the interaction of Alzheimer β-amyloid peptides with gangliosides. J Biol Chem 273:4506-4515
Morgan BL, Winick M. 1980. Effects of administration of N-acetylneuraminic acid (NANA) on brain NANA content and behavior. J Nutr 110:416-424

상세보기
Park CH, Kim SH, Choi W, Lee YJ, Kim JS, Kang SS, Sun YH. 1996. Novel anticholinesterase and antiamnesic activities of dehydroevodiamine, a constituent of Evodia rutaecarpa. Planta Med 62:405-409

상세보기
Park GH, Kim HG, Ju MS, Kim AJ, Oh MS. 2014. Thuja orientalis leaves extract protects dopaminergic neurons against MPTP-induced neurotoxicity via inhibiting inflammatory action. Korea J Herbol 29:27-33
Patel D, Chaudhary SA, Parmar B, Bhura N. 2012. Transdermal drug delivery system: A review. Pharma Innovation 1:66-75
Rastogi V, Yadav P. 2014. Transdermal drug delivery system: An overview. Asian J Pharm 6:161-170
Saulino M, Schengrund CL. 1994. Differential accumulation of gangliosides by the brains of MPTP-lesioned mice. J Neurosci Res 37:384-391

상세보기
Schauer R, Corfield AP. 1982. Isolation and purification of sialic acids. In Schauer R (Ed.), Sialic Acids. pp.51-57. Springer
Schliebs R, Arendt T. 2011. The cholinergic system in aging and neuronal degeneration. Behav Brain Res 221:555-563

상세보기
Selkoe DJ. 2012. Preventing Alzheimer's disease. Science 337: 1488-1492

상세보기
Stefanovic V, Massarelli R, Mandel P, Rosenberg A. 1975. Effect of cellular desialylation on choline high affinity uptake and ecto-acetylcholinesterase activity of cholinergic neuroblasts. Biochem Pharmacol 24:1923-1928

상세보기
Sun L, Xu S, Zhou M, Wang C, Wu Y, Chan P. 2010. Effects of cysteamine on MPTP-induced dopaminergic neurodegeneration in mice. Brain Res 1335:74-82

상세보기
Trabace L, Cassano T, Steardo L, Pietra C, Villetti G, Kendrick KM, Cuomo V. 2000. Biochemical and neurobehavioral profile of CHF2819, a novel, orally active acetylcholinesterase inhibitor for Alzheimer's disease. J Pharmacol Exp Ther 294:187-194

상세보기
Van Cauwenberghe C, Van Broeckhoven C, Sleegers K. 2016. The genetic landscape of Alzheimer disease: Clinical implications and perspectives. Genet Med 18:421-430

상세보기
West BD, Shughrue PJ, Vanko AEH, Ransom RW, Kinney GG. 2006. Amphetamine-induced locomotor activity is reduced in mice following MPTP treatment but not following selegiline/MPTP treatment. Pharmacol Biochem Behav 84:158-161

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