Azizuddin, Azizuddin
(Department of Chemistry, Federal Urdu University of Arts, Science & Technology)
,
Iqbal, Muhammad
(Department of Chemistry, Federal Urdu University of Arts, Science & Technology)
,
Musharraf, Syed Ghulam
(H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi)
,
Shahzad, Saleem
(Department of Agriculture & Agribusiness Management, University of Karachi)
Microbial transformation of a potent progestin, norethisterone (17-hydroxy-19-nor-17α-pregn-4-en-20-yn-3-one) (1) was carried out by using two filamentous fungi Aspergillus niger and Penicillium citrinum. Biotransformation of 1 with A. niger yielded a hydroxylated transformed pr...
Microbial transformation of a potent progestin, norethisterone (17-hydroxy-19-nor-17α-pregn-4-en-20-yn-3-one) (1) was carried out by using two filamentous fungi Aspergillus niger and Penicillium citrinum. Biotransformation of 1 with A. niger yielded a hydroxylated transformed product 10,17-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (2) whereas 11,17-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (3) was obtained through microbial transformation of 1 by P. citrinum. It is the first report of their production from 1 by using A. niger and P. citrinum with complete 1H- and 13C-NMR assignment. The structures of both metabolites were characterized by various spectroscopic techniques and reported data.
Microbial transformation of a potent progestin, norethisterone (17-hydroxy-19-nor-17α-pregn-4-en-20-yn-3-one) (1) was carried out by using two filamentous fungi Aspergillus niger and Penicillium citrinum. Biotransformation of 1 with A. niger yielded a hydroxylated transformed product 10,17-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (2) whereas 11,17-diydroxy-19-nor-17α-pregn-4-en-20-yn-3-one (3) was obtained through microbial transformation of 1 by P. citrinum. It is the first report of their production from 1 by using A. niger and P. citrinum with complete 1H- and 13C-NMR assignment. The structures of both metabolites were characterized by various spectroscopic techniques and reported data.
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제안 방법
the fermentation of 1 with both fungi. For this study, one Erlenmeyer flask of each fungus was harvested, extracted and analyzed by TLC in order to check the degree of transformation of substrate 1 after every 2 days of fermentation. The % degree of transformation of NET (1) into its transformed products 2, 3 and unknown was determined after the purification of compounds (Fig.
대상 데이터
25mm thick, Merck) and were detected under the UV light (254 and 366 nm). The LREI-MS were recorded on Finnigan MAT 311 mass spectrometer with a MASPEC data system while Jeol JMS 600 and HX 110 mass spectrometers with the data system DA 5000 were used to perform HREI-MS. IR spectroscopy was carried out on a Jasco A-302
참고문헌 (14)
Donova, M. V.; Egorova,O. V. Appl. Microbiol. Biotechnol. 2012, 94, 1423-1447.
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