[논문]Effects of nitrogen gas flushing in comparison with argon on rumen fermentation characteristics in in vitro studies

Park, KiYeon; Lee, HongGu

doi:10.5187/jast.2020.62.1.52

Effects of nitrogen gas flushing in comparison with argon on rumen fermentation characteristics in in vitro studies 원문보기

Journal of animal science and technology : JAST, v.62 no.1, 2020년, pp.52 - 57

Park, KiYeon (Department of Animal Science and Technology, Konkuk University) , Lee, HongGu (Department of Animal Science and Technology, Konkuk University)

Abstract ▼ AI-Helper

In rumen in vitro experiments, although nitrogen gas (N2) flushing has been widely used, its effects on rumen fermentation characteristics are not clearly determined. The present study is the first to evaluate the effects of N2 flushing on rumen fermentation characteristics in in vitro batch culture system by comparing with new applicable non-metabolizable gas: argon (Ar). The rumen fluid was taken from two Korean native heifers followed by incubation for 3, 9, 12, and 24 h with N2 or Ar flushing. As a result, in all incubation time, N2 flushing resulted in higher total gas production than Ar flushing (p < 0.01). Additionally, in N2 flushing group, ammonia nitrogen was increased (p 0.05). In conclusion, the present study demonstrated that N2 flushing can influence the rumen nitrogen metabolism via increased ammonia nitrogen concentration and Ar flushing can be used as a new alternative flushing gas.

주제어

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문제 정의

In the present study, argon gas (Ar) was selected as an alternative gas because there is no possibility for Ar to influence the rumen nitrogen metabolism due to its extremely low biological availability and toxicity for microbes. Given the above, the objective of the present study was to investigate the effects of N₂flushing on rumen fermentation kinetics by comparing its effects with Ar flushing using rumen in vitro batch culture system.

제안 방법

999%) N₂ and Ar was flushed into headspace of the serum bottles, respectively. For each treatment, N₂ or Ar flushing, four replications were incubated at 3, 9, 12, and 24 h.
, Seoul, Korea) [14]. To measure the methane concentration, 0.3 mL of head space gas was collected by gas tight syringe (Gastight#1001; Hamilton Co., Reno, NV, USA) and then injected manually to a gas chromatograph (HP 6890 series GC system; Agilent Technologies Inc., Santa Clara, CA, USA) equipped with a thermal conductivity detector and a capillary column (HP-PLOT/Q; Agilent Technologies Inc., Santa Clara, CA, USA). The temperatures of the inlet, oven and detector were 50℃, 50℃, and 250℃, respectively.

대상 데이터

The temperatures of the inlet, oven and detector were 50℃, 50℃, and 250℃, respectively. The helium gas was used as carrier gas. The standard gas with known composition: H₂ 1.
The helium gas was used as carrier gas. The standard gas with known composition: H₂ 1.0%, CH₄ 10.1%, CO₂ 20.1% and N₂ 19.9% in He (MS Dong Min Specialty Gases, Inc., Pyeongtaek, Korea) was used to quantify CH₄ concentration.

데이터처리

Data were analyzed as a two-way ANOVA with source of flushing gases and time as separate factors using general linear model procedure of SAS software (SAS Institute, Cary, NC, USA). The significant differences were accepted if p < 0.

후속연구

The increased ammonia nitrogen in N₂ flushed group demonstrated that N₂ can influence the rumen nitrogen metabolism which can mislead into confounding results. Further studies should be conducted to identify how N₂ flushing affect the rumen nitrogen metabolism and to reinvestigate the effects of CO₂ flushing on rumen fermentation by comparing it with Ar flushing.
Nevertheless, increased NH₃-N still indicates that N₂ flushing influences rumen N metabolism which should be considered when choosing a flushing gas. Therefore, further studies should demonstrate how rumen N metabolism was affected by N₂ flushing (e.g., acetylene reduction assay [24], detecting the nitrogenase activity and its gene expression).

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