[논문]Pezizomycotina (Ascomycota) Fungi Isolated from Freshwater Environments of Korea: Cladorrhinum australe, Curvularia muehlenbeckiae, Curvularia pseudobrachyspora, and Diaporthe longicolla

Nam, Bora; Lee, Jae Sung; Lee, Hyang Burm; Choi, Young-Joon

doi:10.4489/kjm.20200003

Pezizomycotina (Ascomycota) Fungi Isolated from Freshwater Environments of Korea: Cladorrhinum australe, Curvularia muehlenbeckiae, Curvularia pseudobrachyspora, and Diaporthe longicolla 원문보기

한국균학회지 = The Korean journal of mycology, v.48 no.1, 2020년, pp.29 - 38

Nam, Bora (Department of Biology, Kunsan National University) , Lee, Jae Sung (Department of Biology, Kunsan National University) , Lee, Hyang Burm (Division of Food Technology, Biotechnology & Agrochemistry, Chonnam National University) , Choi, Young-Joon (Department of Biology, Kunsan National University)

Abstract ▼ AI-Helper

Fungi are ubiquitous and indispensable components of nearly all ecosystems on earth, including freshwater environments. A survey of fungal diversity in freshwater environments of Korea led to the identification of four unrecorded Pezizomycotina (Ascomycota) species in 2016 and 2017, based on morphology and molecular phylogeny; these included Cladorrhinum australe, Curvularia muehlenbeckiae, Curvularia pseudobrachyspora (Dothideomycetes), and Diaporthe longicolla (Sordariomycetes).

주제어

표/그림 (5)

표 Table 1. Fungal isolates from freshwater environments.
그림 Fig. 1. Minimum evolution tree of Cladorrhinum (A) and Diaporthe species (B) based on the internal transcribed spacer (ITS) rDNA sequences.
그림 Fig. 2. Minimum evolution trees of Curvularia species based on (A) the internal transcribed spacer (ITS) rDNA sequences and (B) glyceraldehyde-3-phosphate dehydrogenase (gpd) gene sequences.
그림 Fig. 3. Morphology of Cladorrhinum australe CNFG 2023 (W80) (A-H) and Diaporthe longicolla CNFG 2025 (W159) (I-P).
그림 Fig. 4. Morphology of Curvularia pseudobrachyspora CNFG 2027 (W263) (A-H) and Curvularia muehlenbeckiae CNFG 3205 (W547) (I-P).

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가설 설정

Description: Three days after inoculation on PDA, colonies floccose, dense, and white with occasional green-yellow areas, but after a week, producing abundant, black clusters of stroma with elongated necks at maturity. Reverse initially colorless and blackish with age.

제안 방법

Cultural characteristics were investigated three days after inoculation at 28℃ in the dark on the following three different media: PDA, V8 agar (V8A; 8% V8 juice [v/v] and 1.5% agar [w/v] adjusted to pH 6.0), and corn meal agar (CMA; Difco, Detroit, MI, USA). Fungal structures formed on the three different media were mounted on a drop of distilled water on a microscope slide and covered with a coverslip.

대상 데이터

The slides were examined and imaged using an Olympus BX53F microscope (Olympus, Tokyo, Japan) equipped with a DigiRetina 16M digital camera (Tucsen, Fuzhou, China). At least 50 units for each structure were measured.
Fungal structures formed on the three different media were mounted on a drop of distilled water on a microscope slide and covered with a coverslip. The slides were examined and imaged using an Olympus BX53F microscope (Olympus, Tokyo, Japan) equipped with a DigiRetina 16M digital camera (Tucsen, Fuzhou, China). At least 50 units for each structure were measured.

이론/모형

, Seoul, Korea). The ITS rDNA sequences were analyzed using the DNAStar software package version 5.05 (DNAStar, Inc., Madison, Wis., USA) and aligned using MAFFT 7 [16] with the Q-INS-i algorithm [17]. The minimum evolution tree was inferred in MEGA6.

성능/효과

Based on the cultural and morphological characteristics of the five isolates (W80, W159, W192, W263, W547), it was confirmed that the two isolates W80 and W159 belonged to Cladorrhinum (Sordariales) and Diaporthe (Diaporthales), respectively, of the class Sordariomycetes. In comparison, the remaining three isolates W192, W263, and W547 belonged to Curvularia (Pleosporales) of the class Dothideomycetes.
Based on the cultural and morphological characteristics of the five isolates (W80, W159, W192, W263, W547), it was confirmed that the two isolates W80 and W159 belonged to Cladorrhinum (Sordariales) and Diaporthe (Diaporthales), respectively, of the class Sordariomycetes. In comparison, the remaining three isolates W192, W263, and W547 belonged to Curvularia (Pleosporales) of the class Dothideomycetes. Molecular sequence analysis was performed to confirm this classification.

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