Sandhibigraha, Sudhansu
(Department of Chemical Engineering, National Institute of Technology)
,
Chakraborty, Sagnik
(School of Energy and Environmental Engineering, Hebei University of Technology)
,
Bandyopadhyay, Tarunkanti
(Department of Chemical Engineering, National Institute of Technology)
,
Bhunia, Biswanath
(Department of Bio Engineering, National Institute of Technology)
Here in this work, a 4-chlorophenol (4-CP)-degrading bacterial strain Bacillus subtilis (B. subtilis) MF447840.1 was isolated from the drain outside the Hyundai car service center, Agartala, Tripura, India. 16S rDNA technique used carried out for genomic recognition of the bacterial species. Isolate...
Here in this work, a 4-chlorophenol (4-CP)-degrading bacterial strain Bacillus subtilis (B. subtilis) MF447840.1 was isolated from the drain outside the Hyundai car service center, Agartala, Tripura, India. 16S rDNA technique used carried out for genomic recognition of the bacterial species. Isolated bacterial strain was phylogenetically related with B. subtilis. This strain was capable of breaking down both phenol and 4-CP at the concentration of 1,000 mg/L. Also, the isolated strain can able to metabolize five diverse aromatic molecules such as 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, 4-nitrophenol, and pentachlorophenol for their growth. An extensive investigation was performed to portray the kinetics of cell growth along with 4-CP degradation in the batch study utilizing 4-CP as substrate. Various unstructured models were applied to evaluate the intrinsic kinetic factors. Levenspiel's model demonstrates a comparatively enhanced R2 value (0.997) amongst every analyzed model. The data of specific growth rate (μ), saturation constant (KS), and YX/S were 0.11 h-1, 39.88 mg/L, along with 0.53 g/g, correspondingly. The isolated strain degrades 1,000 mg/L of 4-CP within 40 h. Therefore, B. subtilis MF447840.1 was considered a potential candidate for 4-CP degradation.
Here in this work, a 4-chlorophenol (4-CP)-degrading bacterial strain Bacillus subtilis (B. subtilis) MF447840.1 was isolated from the drain outside the Hyundai car service center, Agartala, Tripura, India. 16S rDNA technique used carried out for genomic recognition of the bacterial species. Isolated bacterial strain was phylogenetically related with B. subtilis. This strain was capable of breaking down both phenol and 4-CP at the concentration of 1,000 mg/L. Also, the isolated strain can able to metabolize five diverse aromatic molecules such as 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol, 4-nitrophenol, and pentachlorophenol for their growth. An extensive investigation was performed to portray the kinetics of cell growth along with 4-CP degradation in the batch study utilizing 4-CP as substrate. Various unstructured models were applied to evaluate the intrinsic kinetic factors. Levenspiel's model demonstrates a comparatively enhanced R2 value (0.997) amongst every analyzed model. The data of specific growth rate (μ), saturation constant (KS), and YX/S were 0.11 h-1, 39.88 mg/L, along with 0.53 g/g, correspondingly. The isolated strain degrades 1,000 mg/L of 4-CP within 40 h. Therefore, B. subtilis MF447840.1 was considered a potential candidate for 4-CP degradation.
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제안 방법
is the saturation constant (g/L), and S is the substrate concentration (g/L). In this study, GraphPad Prism 5 was applied to resolve the nonlinear equation applying non-linear regression analysis. Numerous kinetic factors, namely, μ, μmax, and KS were computed by fitting the experimental data.
1. The in-depth kinetic investigation was performed to assess the effect of various concentrations of 4-CP on the growth of B. subtilis. The intrinsic kinetic parameters were computed using a non-linear regression analysis with best fit unstructured Han and Levenspiel’s model.
Twenty-five different bacteria were isolated from twenty-five waste water samples which were collected from fifteen different places. The phylogenetic study was performed to identify the most potent 4-CP-degrading strain. Genomic identification showed that the isolated strain was phylogenetically associated amid B.
대상 데이터
This material is based upon work supported by the National Institute of Technology, Agartala, India. Authors would like to acknowledge the National Institute of Technology, Agartala, Ministry of Human Resource and Development, Government of India for Fellowship (0000-0003-4637-991X).
이론/모형
By trial and error method, the experimental data were shown to fit reasonably well in Han and Levenspiel’s model [36] for cell growth and 4-CP utilization.
Experimental data on substrate concentration dependency of the specific growth rate in this study fit well with the simulated data find by using the best fit unstructured Levenspiel’s equation.
Genomic analysis of the isolated strain was performed applying 16S rDNA technique. DNA was collected from the isolated species, and the quality of DNA was assessed on 1.
The distance matrix was prepared via the Ribosomal record along with the highest likelihood phylogenetic tree was created. MEGA 5 was used to find out the evolutionary distance bootstrap data by the Jukes-cantor model of the neighbor-joining technique [16]. The thermodynamic characteristics of the bacterial strain were computed by an online package, (https://cail.
2. The evolutionary background was measured applying the neighbor-joining method [23]. The bootstrap consensus tree anecdotal as replicates of 1,000 [24] was used to signify the evolutionary background of the taxa investigated [23].
The bootstrap consensus tree anecdotal as replicates of 1,000 [24] was used to signify the evolutionary background of the taxa investigated [23]. The evolutionary span was computed applying the Jukes-Cantor technique [25] along with expressed in the units of the numeral of base changeover for each position. The rate difference amongst sites was computed using a gamma distribution (form factor = 1) [16].
The intrinsic kinetic parameters were computed using a non-linear regression analysis with best fit unstructured Han and Levenspiel’s model.
성능/효과
html). Results showed that B. subtilis MF447840.1 has 55% of GC content. Gibbs free energy (ΔG) and enthalpy (ΔH) along with entropy (ΔS) were found to be −2,464.
The results showed that the isolated strain could able to degrade 94.79% of phenol, 84.96% of 2-chlorophenol, 90.90% of 4-CP, 92.82% of 2,4-dichlorophenol, 75.53% of 2,4,6-trichlorophenol, 89.52% of 4-nitrophenol and 90.02% of pentachlorophenol after 48 h of incubation at 37°C.
Information concerning other adjacent homologs for microorganisms may be obtained in the alignment table (Table 1). The species ChE_BE_1 showed phylogenetically adjacent associated to Bacillus subtilis (B. subtilis), species ZAP018-1 (GenBank number: KU587801.1), B. subtilis, strain F3-3 (GenBank number: KT735214.1), B. subtilis, strain PCD3 (GenBank number: KY910140.1), B. subtilis, strain FL39 (GenBank number: KY818957.1), B. subtilis, strain IP18 (GenBank number: KY621529.1), B. subtilis, strain JL02 (GenBank number: KY400283.1), B. subtilis, strain JL12 (GenBank number: KY400272.1), Bacillus tequilensis, strain 7PJ-7 (GenBank number: KR708845.1), B. subtilis (GenBank number: KX058074.1), B. subtilis, strain D12-5 (GenBank number: KT955740.1), B. subtilis, strain VASB19/TS (GenBank number: KT427429.1), B. subtilis, strain 6R3-15 (GenBank number: LC155964.1), Bacterium, strain CDSHGTR2A-21 (GenBank number: KU743239.1) and Bacterium, strain CDSHGTGPM-16 (GenBank number: KU743237.1), and showed 98% sequence match. Hence, the isolated bacterial strain was recognized as B.
참고문헌 (46)
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