[논문]Effect of PVA Concentration on Strength and Cell Growth Behavior of PVA/gelatin Hydrogels for Wound Dressing

Kim, Soyeun; Lim, Hyunju; Kim, Sojeong; Lee, Deuk Yong

doi:10.9718/jber.2020.41.1.1

Effect of PVA Concentration on Strength and Cell Growth Behavior of PVA/gelatin Hydrogels for Wound Dressing 원문보기

Journal of biomedical engineering research : the official journal of the Korean Society of Medical & Biological Engineering, v.41 no.1, 2020년, pp.1 - 7

Kim, Soyeun (Department of Biomedical Engineering, Daelim University) , Lim, Hyunju (Department of Biomedical Engineering, Daelim University) , Kim, Sojeong (Department of Biomedical Engineering, Daelim University) , Lee, Deuk Yong (Department of Biomedical Engineering, Daelim University)

Abstract ▼ AI-Helper

Polyvinyl alcohol (PVA)/gelatin hydrogels were prepared by repeating freezing/thawing three times to evaluate the influence of PVA concentration on the strength and the cell growth behavior of the PVA/gelatin hydrogels. The swelling rate of the PVA/gelatin hydrogels decreased with raising the PVA content from 6 wt% to 12 wt% due to the formation of 3-D network inside the hydrogel. No appreciable degradation of the hydrogels was detected. As the PVA content increased from 6 wt% to 12 wt%, the strength of the PVA/gelatin hydrogels increased drastically from 6.4±0.9 kPa to 46.6±9.0 kPa. The PVA/gelatin hydrogels did not show any evidence of causing cell lysis or toxicity, implying that the hydrogels are clinically safe and effective. Although the strength increased with increasing the PVA content, the PVA/gelatin hydrogels containing 8 wt% exhibited the fastest cell growth, which is highly suitable for wound dressing requiring fast healing regeneration.

주제어

표/그림 (7)

그림 Fig. 1. FT-IR spectra of gelatin, PVA, and 6wt%~12wt% PVA/gelatin specimens
그림 Fig. 2. SEM images of the cross-sections of various PVA/gelatin hydrogels containing different PVA concentration of (a) 6 wt%, (b) 8 wt%, (c) 10 wt%, and (d) 12 wt%
그림 Fig. 3. Compressive strengths of PVA/gelatin hydrogels as a function of PVA content
그림 Fig. 4. Swelling rate of PVA/gelatin hydrogels as a function of PVA concentration
그림 Fig. 5. Photographs of cell morphology: (a) positive control, (b) negative control, and the extracts of (c) 6 wt%, (d) 8 wt%, (e) 10 wt%, and (f) 12 wt% PVA/gelatin hydrogels from EZ-cytox after exposing with the hydrogel suspensions for 48 h
그림 Fig. 6. Proliferation of L-929 cells on the negative control and the PVA/gelatin composite hydrogels with time
그림 Fig. 7. Images of L-929 cell movements from a scratch assay at different time.

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가설 설정

SEM images of the cross-section of PVA/gelatin composite hydrogels are shown in Fig. 2. The crosssectional area of the composite hydrogels appeared nonporous. No appreciable differences between the hydrogels was found.

제안 방법

The PVA/gelatin hydrogels were synthesized by repeating freezing/thawing three times to evaluate the influence of PVA concentration on the physical properties and the cell growth behavior of the hydrogels. Although no appreciable degradation rate was detected, the swelling rate of the hydrogels decreased with raising the PVA content from 6 wt% to 12 wt% due to the formation of 3-D network inside the hydrogel.

대상 데이터

PVA ((CH2CH(OH))n, Mw 85,000~124,000, Sigma-Aldrich Co., USA), gelatin (Daejung Chemicals & Metals Co., Ltd., Korea), and HCl (35%, Junsei Chemical Co., Ltd., Japan) were used as received.

성능/효과

3. As the PVA concentration rose from 6 wt% to 12 wt%, the compressive strength of the hydrogels increased dramatically from 6.4±0.9 kPa to 46.6±9.0 kPa.
The swelling rate of PVA/gelatin hydrogels was also examined by measuring the weight change with time after soaking for 24 h in PBS at room temperature, as shown in Fig. 4. The swelling rate of the PVA/gelatin hydrogels decreased from 26.2% to 14.9% with increasing the PVA concentration from 6 wt% to 12 wt% due to the enhanced formation of 3-dimensional (D) network structure inside the hydrogel. The 3-D network structure is likely to increase with increasing the PVA concentration, leading to the reduction in volume capable of containing water.
To meet practical application needs, the chemical bonds (the esterification reaction) can be adjusted through the adjustment of the PVA concentration. FT-IR results revealed that the esterified PVA/gelatin containing PVA concentration in the range of 6 to 10 wt% had an ester linkage, a secondary alcoholic group, and secondary amide groups.

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