[논문]빈랑자와 천련자 복합물의 만성 역류성 식도염에서 보호 효과

신미래; 이진아; 김민주; 안효진; 노성수

doi:10.6116/kjh.2020.35.1.57.

빈랑자와 천련자 복합물의 만성 역류성 식도염에서 보호 효과
The protective effect of Areca Semen and Toosendan Fructus mixture in a chronic model of reflux esophagitis 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.35 no.1, 2020년, pp.57 - 68

신미래 (대구한의대학교 한의과대학 본초약리학교실) , 이진아 (대구한의대학교 한의과대학 본초약리학교실) , 김민주 (대구한의대학교 한의과대학 본초약리학교실) , 안효진 (상지대학교 한의과대학 약리학교실) , 노성수 (대구한의대학교 한의과대학 본초약리학교실)

Abstract ▼ AI-Helper

Objective : The aim of present study was to clarify the effect of Areca Semen and Toosendan Fructus Mixture (AT-mix) on chronic reflux esophagitis (CRE) in rats. Methods : The antioxidant activity of AT-mix was measured through DPPH and ABTS radical scavenging activities in vitro. CRE was induced in SD rats (5 weeks, male) by ligating the border forestomach and granular portion with 2-0 silk and the duodenum near the pyloric portion was covered with 2-mm wide piece of 18-Fr Nélaton catheter. And then rats were treated AT-mix 200 mg/kg one daily for 14 days. The anti-oxidant and inflammatory protein levels were evaluated using western blotting. Results : Gross lesion of esophageal mucosa after AT-mix treatment showed a superior enhancement compared with that of CRE control rats. AT-mix treatment strongly reduced both DPPH and ABTS radical scavenging activities (DPPH, IC50 8.15±0.14 ㎍/mL; ABTS, IC50 24.69±0.03 ㎍/mL, repspectively). Levels of the NADPH oxidase subunit including NOX4 and p22phox increased in CRE control rats. Otherwise, AT-mix treatment significantly reduced. The activation of Nuclear factor-erythroid 2-related factor 2 (Nrf2) led to significantly the up-regulation of HO-1. The inhibition of IκBα phosphorylation led to NF-κB inactivation. Subsequently, NF-κB inactivation significantly induced the decrease of COX-2, iNOS, TNF-α, and IL-6 protein expressions. Conclusion : Taken together, these results suggest that AT-mix treatment can attenuate the esophageal mucosal ulcer though inhibiting NF-κB pathway and enhancing Nrf2/HO-1 pathway. Thus, the additional mechanism study about AT-mix would need for the development as a safe herbal therapy for CRE.

주제어

표/그림 (6)

그림 Figure 1. DPPH and ABTS radical scavenging activities.
그림 Figure 2. Representative image in each group of esophageal mucosal ulcer after surgical induction of CRE.
그림 Figure 3. Esophageal NOX4 and p22^phox protein expressions.
그림 Figure 4. Esophageal Nrf2, HO-1, SOD, Catalase, and GPx-1/2 protein expressions.
그림 Figure 5. Esophageal p-IκBα and NF-κBp65 protein expressions.
그림 Figure 6. Esophageal COX-2, iNOS, TNF-α, IL-6, and IL-1β protein expressions.

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문제 정의

In summary, the current study demonstrated that AT-mix may exert anti-inflammatory activity by suppressing the NF-κB inflammatory pathways and activating the Nrf2/HO-1 pathway to reduce the release of inflammatory factors such as COX2, iNOS, TNF-α, IL-6, and IL-1β. To the best of our knowledge, this is the first report of the anti-inflammatory activity of AT-mix in a chronic model of reflux esophagitis. These results can indicate that it is possible to develop AT-mix into an effective anti-inflammatory medicine.

제안 방법

All animals had an operation adjustment for 7 days after surgery. At 21 days (an operation adjustment period; 7 days + drug treatment period; 14 days) after surgery, rats were sacrificed and the esophageal tissues were obtained for further processing and analysis. Rats divided into three groups.

대상 데이터

Animal experiments were carried out according to the “Guidelines for Animal Experimentation” approved by the Ethics Committee of the Daegu Haany University with certificate number DHU2019-129. Four-week-old male Sprague-Dawley rats (B.W. 135 - 145 g) were purchased from Daehan Bio Link (Eumseong, Korea). Rats were maintained under a 12-h light/dark cycle, housed at a controlled temperature (24±2ºC) and humidity (about 60%).
(Osaka, Japan). Phenyl methyl sulfonyl fluoride (PMSF) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The pierce bicin choninic acid (BCA) protein assay kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA).

성능/효과

SOD interacts with superoxide radical (O₂⁻) to form H₂O₂ , which is subsequently catabolised by Catalase or GPx to H₂O. In this study, the oral administration of AT-mix significantly increased compared with CRE control rats, otherwise all three of SOD, Catalase, and GPx showed an increased tendancy without a significance. This suggests that the treatment of AT-mix may effectively scavange ROS though up-regulating the expression of HO-1 via Nrf2 activation.

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저자의 다른 논문 :

표제어: PCR

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