[논문]In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals

Jiratchayamaethasakul, Chanipa; Ding, Yuling; Hwang, Ouibo; Im, Seung-Tae; Jang, Yebin; Myung, Seung-Won; Lee, Jeong Min; Kim, Hyun-Soo; Ko, Seok-Chun; Lee, Seung-Hong

doi:10.1186/s41240-020-00149-8

In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals 원문보기

Fisheries and aquatic sciences, v.23 no.3, 2020년, pp.6.1 - 6.9

Jiratchayamaethasakul, Chanipa (Department of Pharmaceutical Engineering, Soonchunhyang University) , Ding, Yuling (Department of Pharmaceutical Engineering, Soonchunhyang University) , Hwang, Ouibo (Department of Pharmaceutical Engineering, Soonchunhyang University) , Im, Seung-Tae (Department of Pharmaceutical Engineering, Soonchunhyang University) , Jang, Yebin (Department of Pharmaceutical Engineering, Soonchunhyang University) , Myung, Seung-Won (Department of Pharmaceutical Engineering, Soonchunhyang University) , Lee, Jeong Min (Department of Applied Research, National Marine Biodiversity Institute of Korea) , Kim, Hyun-Soo (Department of Applied Research, National Marine Biodiversity Institute of Korea) , Ko, Seok-Chun (National Marine Bio-Resources and Information Center, National Marine Biodiversity Institute of Korea) , Lee, Seung-Hong (Department of Pharmaceutical Engineering, Soonchunhyang University)

Abstract ▼ AI-Helper

Background: Halophyte plant (HPs), a salt-resistant flora, has been reported to provide several health benefits, but the knowledge of its cosmeceutical potential is still ambiguous. Here, 70% ethanol extracts of 22 HPs collected from along the coast of South Korea were investigated for their potentials of antioxidant, anti-aging, and whitening properties for use as materials in novel cosmeceuticals. Methods: Antioxidant activities were determined by DPPH (1,1-diphenyl-2-pricrylhydrazyl) free radical and hydrogen peroxide scavenging assays, and skin aging-related enzyme activities (anti-elastase, anti-collagenase, anti-hyaluronidase, and anti-tyrosinase) were evaluated by using the spectrophotometric method. Results: Among the 22 HPs, we found that Ischaemum antephoroides f. coreana and Atriplex gmelinii extracts presented the strongest scavenging effects against DPPH free radical and hydrogen peroxide, respectively. Our finding additionally suggested that Salicornia europaea extract might provide a major source of anti-elastase and anti-hyaluronidase; meanwhile, Rosa rugosa extract showed the highest anti-collagenase effect. Furthermore, the highest tyrosinase inhibitory activity was possessed by Spartina anglica extract. Conclusion: These findings may suggest that halophyte plants showing biological activities may be potent inhibitors of tyrosinase, elastase, collagenase, and hyaluronidase and could be useful for application in cosmeceuticals.

주제어

표/그림 (4)

표 Table 1 Scientific name of HPs used in this study
표 Table 2 DPPH free radical and hydrogen peroxide scavenging activities of 22 HP extracts
표 Table 3 Skin aging-related enzyme inhibitory effects of 22 HP extracts
표 Table 4 Tyrosinase inhibitory effect of 22 HP extracts

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문제 정의

It is vital to note that our screening results are the preliminary demonstration of antioxidant and anti-aging activities. However, to introduce it as a candidate in cosmeceutical application, a future study is needed.

제안 방법

CJ, YD, JML, HSK, SCK, and SHL constituted and designed the experimental plan. CJ, OH, STI, YJ, and SWM conducted the experimental work.
In this study, there are two antioxidant methods to assess antioxidant activities which are DPPH free radical and hydrogen peroxide scavenging assays. As depicted in Table 2, the antioxidant activities were performed and diluted to achieve the final concentration at 0.

대상 데이터

CJ, YD, JML, HSK, SCK, and SHL constituted and designed the experimental plan. CJ, OH, STI, YJ, and SWM conducted the experimental work. CJ wrote the manuscript.

이론/모형

Elastase inhibition was determined by measuring the intensity of the solution color assayed using the method of Tu and Tawata (2015) with slight modifications. The mixture of AAAPVN elastase substrate in 0.

성능/효과

All 22 HP extracts were possessed antioxidant activities to a varying degree ranging from 11.39 ± 5.99% to 93.32 ± 0.46% and 3.95 ± 2.65% to 79.28 ± 1.40% of DPPH free radical and hydrogen peroxide assays, respectively.
Among 22 HPs extracts, I. coreana extract exhibited the highest DPPH free radical scavenging effect (93.32 ± 0.46%) whereas of which 51.14 ± 3.86% hydrogen peroxide scavenging activity was observed.
Apart from R. rugosa extract, there were other two of all examined HP extracts that exhibited fairly high activities in both collagenase and elastase inhibitory effects with 62.24 ± 0.55% and 74.47 ± 0.18% of A. sibirica extract together with 52.86 ± 0.50% and 52.86 ± 0.50% of C. glaucum extract, respectively.
Furthermore, the next strongest antioxidant capacities (> 80%) expressed by DPPH free radical assay were 82.22 ± 0.23% of S. komarovii extract, 82.90 ± 0.23% of C. soldanella extract, 84.52 ± 1.39% of R. rugosa extract, and 84.84 ± 1.05% of A. princeps extract.
anglica extract may be an available source of tyrosinase inhibition. In conclusion, all of these results may recommend the guidance of conceivable HPs in terms of antioxidant, anti-wrinkle, and whitening agent for cosmeceutical development. It is noteworthy that the supplementary experiments of examined HPs such as cell-based experiments and the adverse effects should be more investigated.
In contrast, S. europaea extract possessed the highest hyaluronidase inhibition up to 72.70 ± 1.24% and the significant inhibitions were demonstrated by A. princeps, S. marina, and C. glaucum extracts represented at 51.71 ± 0.33%, 57.73 ± 4.09%, and 57.73±2.36%, respectively.
In our results, 5 of 22 HPs (S. komarovii, A. princeps, I. coreana, C. soldanella, and R. rugosa, extracts) showed comparatively strong antioxidant activities between 82.90 ± 0.23% and 93.32 ± 0.46% inhibition measured by DPPH assay.
Meanwhile, the highest hydrogen peroxide scavenging effect was found by A. gmelinii extract at 79.28 ± 1.40% and the comparative scavenging effect (> 60%) were found by S. marina and C. glaucum extracts showing 65.91 ± 3.91% and 64.32 ± 8.30%, respectively.
Moreover, there were 5 HPs that exhibited good inhibitory effects (> 50%) noticed by A. sibirica (58.16 ± 7.24%), followed by P. japonicum (54.74 ± 0.54%), S. glauca (52.85 ± 0.00%), S. maritima (52.03 ± 3.45%), and A. gmelinii extract (50.77 ± 0.00%).
30%, respectively. The percentages against hydrogen peroxide of all HP extracts showed the lower scavenging effects when compared with that of DPPH free radical except S. marina, C. glaucum, and A. gmelinii (Table 2).

후속연구

It is vital to note that our screening results are the preliminary demonstration of antioxidant and anti-aging activities. However, to introduce it as a candidate in cosmeceutical application, a future study is needed. Further investigations including 50% inhibitory concentration (IC50) and in vitro cell-based experiment, chemically, biologically, and pharmaceutically are required.

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