[논문]옻칠의 건조속도 향상을 위한 효소첨가제 적용 연구

PARK, Ji Hyeon; PARK, Jung Hae; KIM, Soo Chul

doi:10.5658/wood.2020.48.3.326

[국내논문] 옻칠의 건조속도 향상을 위한 효소첨가제 적용 연구
A Study on Application of Enzyme Additives to Improve Drying Speed of Urushi Lacquer 원문보기

목재공학 = Journal of the Korean wood science and technology, v.48 no.3, 2020년, pp.326 - 344

PARK, Ji Hyeon (Department of Heritage conservation and Restoration, Graduate School of Cultural Heritage, Korea National University of Cultural Heritage) , PARK, Jung Hae (Department of Heritage conservation and Restoration, Graduate School of Cultural Heritage, Korea National University of Cultural Heritage) , KIM, Soo Chul (Department of Conservation Science, Korea National University of Cultural Heritage)

초록
AI-Helper

옻칠의 건조속도를 향상시키기 위해 Laccase를 적용하여 효소첨가제로써의 가능성을 확인하고자 하였다. 옻칠의 Laccase와 부후균의 Laccase를 적용한 경우 국내산과 중국산 모두 건조시간이 단축되었다. Laccase를 적용한 시험편 모두 건조시작 60분 안에 지촉건조가 이루어졌으며, Laccase의 함량비율이 높아질수록 건조속도가 향상되었다. 또한 국내산 옻칠과 중국산 옻칠의 경화건조시간의 차이는 있지만 온도 20 ± 2 ℃, 습도 55-60 %의 상온에서도 경화건조가 이루어지는 것이 확인되었다. 건조된 시험편의 도막분석 결과, 우루시올 대비 Laccase 함량비율이 5:1일 때 건조속도가 향상되었으며, 색차와 밀착력의 차이가 없고 도막이 안정적임을 확인하였다. Laccase를 적용한 경우 국내산과 중국산 옻칠의 광택도가 모두 감소되는 양상이 관찰되었으나 이외 FT-IR과 Py-GC/MS의 분석결과 물성에 영향을 미치는 정도의 성분변화는 나타나지 않아 첨가제로써의 적용 가능성을 확인하였다.

Abstract ▼ AI-Helper

Laccase was applied to improve the drying speed of urushi lacquer to confirm a potential use of laccase as an enzyme additive. As a result of applying laccase of lacquer and white-rot fungi, drying times for both Korean and Chinese specimens were reduced. All of the specimens to which laccase was applied were dried(set to touch) within 60 minutes from the start of the drying, and the drying rate was improved as the content ratio of laccase increased. In addition, although there were differences in hardening drying time between Korean and Chinese lacquers, it was confirmed that hardening drying took place even at room with temperature of 20 ± 2 ℃ and humidity of 55-60%. As a result of lacquer layer analysis of the dried specimens, the drying speed was improved when the content ratio of laccase to urushiol was 5:1, and there were no differences in chromaticity and adhesion, confirmed that the layers were stable. It was observed that the gloss of both Korean and Chinese lacquers were reduced when laccase was applied. However, according to the analysis of FT-IR and Py-GC/MS, the changes in components were not as sufficient to affect the physical properties. Thus, its applicability as an additive was confirmed.

주제어

표/그림 (14)

표 Table 1. Composition proportion of lacquer by place of production
그림 Fig. 1. Process of separating components of lacquer.
그림 Fig. 2. A process of making samples.
표 Table 2. List of samples by proportion of Urushiol to Laccase
표 Table 3. Hardening drying time of samples applied on laccase of lacquer (room temperature condition)
표 Table 4. Hardening drying time of samples on the proportion of urushiol and laccase (min)
표 Table 5. Color differences (ΔE*) and degree of gloss (G) after drying of sample applied to the laccase of lacquer (Third)
표 Table 6. Color differences (ΔE*) and degree of gloss (G) after drying of sample applied to the laccase of white-rot fungi (Third)
표 Table 7. Photomicrographs dried sample of surface (laccase of lacquer)
표 Table 8. Photomicrographs dried sample of surface (laccase of white-rot fungi)
표 Table 9. Photomicrographs of cross section of film samples coated with laccase of lacquer
표 Table 10. Photographs before and after adhesion test
그림 Fig. 3. Total FT-IR spectrum of Korean samples in fingerprint area (laccase of lacquer).
그림 Fig. 4. Total FT-IR spectrum of Chinese sample in fingerprint area (laccase of white-rot fungi).

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

From the dried lacquer specimens, sections of the three times coatings were collected to prepare thin slices. After placing the slices on a slide glass, 50% glycerin aqueous solution was dropped, and the slices were covered with a cover glass to produce microscopic observations. The cross sections of the layers on the preparat was observed by using an optical microscope (Eclipse Lv 100, Nikon, Japan) (Kim et al.
In this study, laccases of lacquer and white-rot fungi were applied to confirm the possibility of its usage as an enzyme additive for improve the drying speed of lacquer. When laccase was applied to both Korean and Chinese lacquers, the drying time of the specimen with laccase applied was shorter than that of the specimen without laccase addition.
Therefore, this study aims to apply laccase as an enzyme additive, supplements the differences in composition by region and improves the drying speed in order to enhance the accessibility of using the natural material of lacquer. In addition, it also aims to investigate the stability of additives through the analysis of lacquer layers, so that it could be used in conserving the lacquerware cultural properties.

이론/모형

The adhesion of the dried specimens were evaluated by using ‘KS M ISO 2409, paint and varnish-paint adhesion test method’.

성능/효과

As a result of applying laccase of the lacquer, it was confirmed that the drying rate was improved and no changes in the surface was observed with the naked eyes when the proportion of laccase to urushiol was 5:1. Therefore, the proportion of laccase to urushiol at 5:1 was judged to be the most stable condition.
Based on the chromaticity of original specimen A, the ΔE* values of specimens with second coatings of laccase of the lacquer and those values of the specimens with third coatings were both less than 3, which were not significant enough to be recognized as a difference in color (Table 5).
The results of the room temperature condition drying experiment showed that although there was a difference in hardening drying time between the Korean and Chinese spceimens, it was confirmed that hardening drying performed even in the room with temperature of 20 ± 2 ℃ and humidity of 55-60% (Table 3).

후속연구

Therefore, this study aims to apply laccase as an enzyme additive, supplements the differences in composition by region and improves the drying speed in order to enhance the accessibility of using the natural material of lacquer. In addition, it also aims to investigate the stability of additives through the analysis of lacquer layers, so that it could be used in conserving the lacquerware cultural properties.
However, according to the results of FT-IR and Py-GC/MS analysis, the changes in components were not as sufficient to affect the physical properties, suggesting that it can be applied as an additive. Thus, the results of this study suggest that the accessibility of laccase as an additive was confirmed and using lacquer for the conservation of lacquerware cultural properties could be increased, and an authentic conservation could be achieved.

참고문헌 (23)

Choi, J.W., Kim, S.C. 2018. Analysis of lacquer and CNSL using infrared spectrometer and pyrolysis- GC/MS. Journal of the Korean Wood Science and Technology 46(1): 1-9.
Do, C.H., Lee, T.Y. 1999. Lacquer coatings on the koryo buddhist canon printing wood blocks. Journal of Conservation Science 8(1): 33-39.
Han, J.S. 2014. Diversification and mass production of traditional lacquer. Ministry of Culture, Sports and Tourism Standardization pp.3-11.
Hidayat, W., Qi, Yue., Jang, J.H., Park, B.H., Banuwa, I.S., Febrianto, F., Kim, N.H. 2017. Color change and consumer preferences towards color of heattreated Korean white pine and royal paulownia woods. Journal of the Korean Wood Science and Technology 45(2): 213-222.

원문보기 상세보기
Hwang, I.S., Park, J.H., Kim, S.C. 2018. A study on the optical characteristics according to the lacquer drying conditions for the conservation of lacquerwares. Journal of the Korean Wood Science and Technology 46(5): 610-621.

원문보기 상세보기
Jeong, I.B., Yoon, H.D., Maeng, J.S., Kang, S.W., Ha, Y.C., Jeong, G.J., Choi, H.T., Kim, J.H. 1992. Characteristics of lacase secreted by Lentinus edodes. The Korean Journal of Microbiology 30(4): 252-259.
Jung, H.C. 2019. Enhancement of laccase production from wood-rotting fungus by co-culture with trichoderma longibrachiatum. Journal of the Korean Wood Science and Technology 47(2): 210-220.

원문보기 상세보기
Kim, K.J., Shin, K.S., Maeng, J.S., Kang, S.O., Hah, Y.C., Hong, S.W. 1987. Characterization of laccase from Pleurotus ostreatus. The Korean Journal of Microbiology 25(2): 148-156.
Kim, S.C. 2007. Analysis and conservation of lacquer wares from archaeological sites in Korea. Doctoral thesis, Chungbuk National University, Cheongju.
Kim, S.C., Lee, G.H., Kang, H.T., Shin, S.P., Han, M.S. 2010. Lacquer manufacturing method for the wooden coffins of king muryeong's tomb based on the scientific analysis. Journal of Conservation Science 26(3): 247-258.
Kim, J.E. 2016. A study of adhesion characteristics and manufacturing techniques for lacquer with additives. Master thesis, Graduate School of Cultural Heritage, Korea National University of Cultural Heritage, Buyeo.
Lee, P.W., Kim, H.J. 1990. Characteristics of hardening behavior of Korean Rhus Lacquer by F.T.-I.R. Journal of the Korean Wood Science and Technology 18(1): 10-14.

원문보기 상세보기
Lee, S.J. 2017. Conservation Ethics, Graphic Korea, pp.61-77, Seoul.
Lee, Y.H. 2010. A study of the techniques of the ancient korean lacquer wares. Master thesis, Kongju National University, Gongju.
Park, S.J., Lee, J.Y., Cho, N.S., Cho, B.M. 1993. Wood science experiment book, Gwangil Cultural Company, pp.623-653, Seoul.
Park, S.Y., Kim, J.C., Kim, J.H., Yang, S.Y., Kwon, O.K., Yeo, H.M., Cho, K.C., Choi, I.G. 2017. Possibility of wood classification in Korean softwood species using near-infrared spectroscopy based on their chemical compositions. Journal of the Korean Wood Science and Technology 45(2): 202-212.

원문보기 상세보기
Park, J.S., Ahn, S.A. 2018. Analysis of dried Asian lacquer by pyrolysis/GC/MS. Analytical Science and Technology 31(2): 78-87.
Roh, J.K., Kim,Y.G. 2008. Grade classification of urushi lacquer(II) grade classification of urushi lacquer by scientific methods. Journal of the Korea Furniture Society 19(5): 307-318.
Son, D.H. 2006. Traditional lacquer craft. Korea Cultural Properties Protection Foundation, pp.8-54.
Song, H.K., Han, C.H. 2001. Study on refining technique of raw lacquer (2): Refining and mechanical properties of refined lacquer according as mixing rate of components. Journal of the Korean Wood Science and Technology 29(1): 43-51.
Shin, S.P. 2008. The evaluation of conservation characteristics on lacquer coats by the reproduction experiment. Master thesis, Kongju National University, Gongju.
Won, C.H., Lee, S.Y., Baek, Y.J. 2004. Degradation of PAHs using Laccase from Trametes sp. Journal of Environmental Science and Technology 5(1): 43-48.
Yeon, S.H., Park, S.Y., Kim, J.H., Kim, J.C., Yang, S.Y., Yeo, H.M., Kwon, O.K., Choi, I.G. 2019. Effect of organic solvent extractives on Korean softwoods classification using near-infrared spectroscopy. Journal of the Korean Wood Science and Technology 47(4): 509-518.

원문보기 상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증