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제안 방법
- Therefore, to investigate the effects of ATE on adipogenic differentiation, 3T3-L1 preadipocytes were treated with various concentrations of ATE (0, 100, 300, and 500 μg/mL).
- 09%, without affecting total mTOR expression. To further investigate the upstream mechanism of ATE in adipogenesis, we confirmed the IRS/PI3K/AKT signaling pathway proteins, which constitute the upper mechanism of mTOR signaling. Proteins expression levels were determined via western blot analysis.
- mTOR has been reported to be involved in adipogenesis, and regulates the expression of PPARγ.23) Therefore in this study, we further evaluated mTOR, an upstream mechanism of C/EBPα and PPARγ.
대상 데이터
- Dulbecco’s modified Eagle’s medium (DMEM), Bovine Calf Serum (BCS), and penicillin-streptomycin were purchased from Gibco BRL (Grand Island, NY, USA). Fetal Bovine Serum (FBS) was purchased from ATLAS Biologicals (Fort Collins, CO). The 3-isobutylmethylxanthine, insulin, and dexamethasone were obtained from Wako Pure Chemical Industries Ltd.
- The 3T3-L1 mouse preadipocytes were purchased from the American Type Culture Collection (Rockville, MD, USA), and maintained in DMEM containing 10% BCS with 1% penicillin-streptomycin at 37 ℃ in an incubator containing a humidified atmosphere with 5% CO2 . To differentiate 3T3-L1 preadipocytes into mature adipocytes (defined as Day 0), fully confluent 3T3-L1 preadipocytes were treated with differentiation medium containing DMEM, 10% fetal bovine serum, 0.
데이터처리
- The values were compared using Student’s t-test. One-way analysis of variance (ANOVA) was conducted using the software Origin 7 (Microcal Software, USA). Values of P < 0.
- The values were compared using Student’s t-test.
이론/모형
- Cell viability was determined by MTT assay. The cells were treated with various concentrations of ATE (0, 100, 300, and 500 μg/mL) for 24 h.
- (A) 3T3-L1 cells were treated with various concentrations of ATE (0, 100, 300, and 500 μg/mL) for 24 h. The cell viability was evaluated by MTT assay, as described in the Materials and methods. (B) Lipid droplets were photographed after Oil-Red O staining, and lipid content was then quantified by measuring absorbance.
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