[논문]Zinc Undecylenate가 말똥성게(Hemicentrotus pulcherrimus)와 둥근성게(Mesocentrotus nudus)의 배아발생에 미치는 독성 영향

최훈; 박윤호; 이주욱; 이승민; 최윤석; 황운기

doi:10.23005/ksmls.2020.5.2.43

Zinc Undecylenate가 말똥성게(Hemicentrotus pulcherrimus)와 둥근성게(Mesocentrotus nudus)의 배아발생에 미치는 독성 영향
Toxic Effect of Zinc Undecylenate on the Embryogenesis of Sea Urchins Hemicentrotus pulcherrimus and Mesocentrotus nudus 원문보기

한국해양생명과학회지 = Journal of marine life science, v.5 no.2, 2020년, pp.43 - 50

최훈 (국립수산과학원 서해수산연구소 해양생태위해평가센터) , 박윤호 (국립수산과학원 서해수산연구소 해양생태위해평가센터) , 이주욱 (국립수산과학원 서해수산연구소 해양생태위해평가센터) , 이승민 (국립수산과학원 서해수산연구소 해양생태위해평가센터) , 최윤석 (국립수산과학원 서해수산연구소 해양생태위해평가센터) , 황운기 (국립수산과학원 서해수산연구소 해양생태위해평가센터)

초록
AI-Helper

본 연구에서는 살균제, 항진균제 등의 의약품을 포함하여 다양한 목적으로 사용되며, 신방오도료로서의 가능성이 확인된 바 있는 Zinc undecylenate (ZU)를 이용해 연안환경 내 1차 소비자를 대표할 수 있는 성게 2종(H. pulcherrimus, M. nudus)에 대한 독성평가를 실시하였다. 실험결과 ZU에 대한 H. pulcherrimus와 M. nudus의 수정률 EC₅₀은, 각각 11.27 mgl^-1과 1.48 mgl^-1로 나타났다. 또한, 정상배아 발생률의 EC₅₀은 각각 0.94 mgl^-1와 3.78 mgl^-1로 나타났으며, NOEC는 0.20 mgl^-1, 0.78 mgl^-1를 나타내었다. 본 연구에서 도출된 성게 2종과 문헌조사를 통한 연안양식생물 2종의 급성독성결과를 이용하여 Predicted No Effect Concentration (PNEC)를 계산하였다. PNEC 값은 0.0094 mgl^-1로 나타났으며, 위와 같은 결과는 해양환경 오염물질에 대한 환경보호전략 수립을 위한 기초자료로 활용될 것이다.

Abstract ▼ AI-Helper

The aim of this study is toxicity assessment using two types of sea urchins (H. pulcherrimus, M. nudus) that can representative primary consumers in potential coastal environments pollutants, Zinc undecylenate (ZU), which is used for various purposes, such as pharmaceutical agents and anti-bacterial and anti-fungi. The Fertilization rate and normal embryogenesis rate of H. pulcherrimus and M. nudus were concentration-dependent decreased. Besides, EC50 of fertilization rate with H. pulcherrimus and M. nudus were 11.27 mgl-1 and 1.48 mgl-1, and EC50 of normal embryogenesis were 0.94 mgl-1 and 3.78 mgl-1. NOEC of normal embryogenesis were 0.20 and 0.78 mgl-1, respectively. In addition, to find the safety criteria of the ZU on the marine environment. PNEC value was 0.0094 mgl-1, calculated using the toxicity values of two species of sea urchin derived from this study and the acute toxicity results of the coastal area through literature research. The above results will be used as basic data for establishing environmental protection strategies for marine environmental pollutants.

주제어

표/그림 (6)

표 Table 1. Information of the Zinc undecylenate and experimental design used in this study
표 Table 2. Information of experimental design used in this study
그림 Fig. 1. Normal fertilized egg (A, C) and abnormal fertilized egg (B, D), of H. pulcherrimus and M. nudus, respectively. Normal and abnormal ferilized egg is determined by the presence or absence of fertilized membrane which is indicated in (A). The fertilized eggs were development to Pluteus larvae through various stages, such as 4-cells (E), Morular (F), Blasftula (G), Gastrula (H) in both sea urchin species. Normal pluteus larva (I, K) and abnormal development larva (J, L) of H. pulcherrimus and M. nudus, respectively. Normal and abnormal pluteus larvae were determined by the skeletogenesis, especially, development.
그림 Fig. 2. Changes of fertilization rate on two Sea urchin species, H. pulcherrimus and M. nudus exposed to Zinc undecylenate (*p<0.05, **p<0.01).
그림 Fig. 3. Change of normal embryogenesis rate of H. pulcherriums and M. nudus exposed to Zinc undecylenate for h and 48 h, respectively (**p<0.01).
표 Table 3. List of Zinc undecylenate toxicity tests using various marine organisms. PNEC (Predicted No Effect Concentration) values follow by calculated using the various toxicity values get through litterateur study

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저자의 다른 논문 :

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