[논문]담수산다슬기, Semisulcospira coreana의 열충격단백질 유전자 특성 및 발현분석

박승래; 최영광; 이화진; 이상윤; 김이경

doi:10.23005/ksmls.2020.5.1.17

담수산다슬기, Semisulcospira coreana의 열충격단백질 유전자 특성 및 발현분석
Characterization of Heat Shock Protein 70 in Freshwater Snail, Semisulcospira coreana in Response to Temperature and Salinity 원문보기

한국해양생명과학회지 = Journal of marine life science, v.5 no.1, 2020년, pp.17 - 24

박승래 (강릉원주대학교 해양생물공학과) , 최영광 (강릉원주대학교 해양생물공학과) , 이화진 (강릉원주대학교 해양생물공학과) , 이상윤 (강릉원주대학교 동해안생명과학연구원) , 김이경 (강릉원주대학교 해양생물공학과)

초록
AI-Helper

참다슬기 아가미 조직으로부터 heat shock protein 70 유전자를 분리·동정하였다. 참다슬기 HSP70 cDNA의 open reading frame (ORF)는 1,917 bp로 639개의 아미노산을 암호화하여 분자량은 약 70 kDa으로 예측되었다. 생물정보학 배열분석에 의해 HSP 유전자 기능과 관여되어 있는 3가지 주요 signature motifs와 보존된 도메인을 확인하였다. 계통학적 분석을 통하여 참다슬기 HSP70 유전자는 왕우렁이 Pomacea canaliculate와 같은 클러스트에 포함된다는 사실을 확인하였다. 수온 및 염분 변화에 따라, 참다슬기 HSP70 mRNA 유전자 레벨은 유의적으로 증가하였으며(p < 0.05), 이는 외부자극요인을 파악할 있는 분자생물학적 마커로서 활용될 수 있을 것으로 사료된다.

Abstract ▼ AI-Helper

We have identified a heat shock protein 70 gene from freshwater snail, Semisulcospira coreana. The freshwater snail HSP70 gene encode a polypeptide of 639 amino acids. Based on bioinformatic sequence characterization, HSP70 gene possessed three classical signature motifs and other conserved residues essential for their functionality. The phylogenetic analysis showed that S. coreana HSP70 had closet relationship with that of golden apple snails, Pomacea canaliculata. The HSP70 mRNA level was significantly up-regulated in response to thermal and salinity challenges. These results are in agreement with the results of other species, indicating that S. coreana HSP70 used be a potential molecular marker in response to external stressors and the regulatory process related to the HSP70 transcriptional response can be highly conserved among species.

주제어

표/그림 (6)

그림 Fig. 1. Nucleotide and deduced amino acid sequence of Semisulcospira coreana HSP70. The conserved three signature motifs are shaded.
그림 Fig. 2. Multiple amino acid sequence alignments of S. coreana HSP70 along with those from other selected species. Asterisks indicate identical residues and gaps introduced for optimum alignments, respectively, The three signature motifs, the putative ATP-GTP binding domain (AEAYLG) and nonorganelle eukaryotic consensus motifs (RARFEEL) are upperlined.
표 Table 1. Protein sequence identities of freshwater snail HSP70 with other orthologues
그림 Fig. 3. Phylogenetic tree of HSP70. The freshwater snail, S. coreana sequences cloned in the present study are indicated by thick black arrows. The neighbor-joining method with 1,000 bootstrap replications was used to generate the tree.
그림 Fig. 4. The mRNA expression of scHSP70 in gill tissue of S. coreana after heat challenge by qPCR method. The experiment was performed in triplicate, and different letters indicate significant differences at p<0.05.
그림 Fig. 5. The mRNA expression of scHSP70 in gill tissue of S. coreana after salinity challenge by qPCR method. The experiment was performed in triplicate, and different letters indicate significant differences at p<0.05.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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