[논문]The Improvement of skin barrier function and anti-obesity effect of Codonopsis lanceolata by supercritical carbon dioxide extraction

Kim, Bora

doi:10.12925/jkocs.2020.37.4.674

The Improvement of skin barrier function and anti-obesity effect of Codonopsis lanceolata by supercritical carbon dioxide extraction 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.37 no.4, 2020년, pp.674 - 681

Kim, Bora (Division of Chemistry and Cosmetics, Mokwon University)

Abstract ▼ AI-Helper

The root of Codonopsis lanceolata has been used in traditional medicine. This study was conducted to confirm the comparative effect of ethanol solvent extraction (CLE) and supercritical carbon dioxide extraction (CLS) of C. lanceolata roots. CLS had higher antioxidant than CLE. For supercritical co-solvent modified carbon dioxide extraction (CLS), it were extracted at 250 bar 50℃ 150 min at a flow rate of ethyl alcohol 3 mL/min for 90min. In addition, CLS inhibited the adipocyte differentiation of 3T3-L1 cells. When treated with the extract at a concentration of 100 ㎍/mL, the Wnt/β-catenin pathway reporter luciferase activity of HEK 293-TOP cells increased approximately by 3-folds compared to that of the untreated control group. Also, the treatment by CLS (50 ㎍/mL) showed a significant increase of involucrin expression. These results indicate that supercritical carbon dioxide extract of C. lanceolatamay serve as a cosmeceutical agent for improving skin barrier function and the treatment of obesity.

주제어

표/그림 (6)

그림 Fig. 1. Effect of CL on cell viability.
그림 Fig. 2. Antioxidant effect on CL.
그림 Fig. 3. Effect of CLS on Luciferase reporter activity.
그림 Fig. 4. Effect of CLS on the adipocyte differentiation of 3T3-L1 preadipocyte cells.
그림 Fig. 5. Effect of C. lanceolata extract on expression of involucrin.
그림 Fig. 6. Immunohistochemical analysis of involucrin in 3D skin equivalent model.

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

However, the effects of its extract as a skin therapeutic agent for skin barrier function and as a nutraceutical product for obesity treatment have not been studied. This study aims to verify the effect of C. lanceolata extract on strengthening the skin barrier and suppressing obesity.
Also, the treatment by CLS (50 µg/mL) showed a significant increase of involucrin expression. This study suggests that CLS have potential as cosmeceutical agents for improving skin barrier functions and the treatment of obesity.

대상 데이터

The C. lanceolata was purchased from the Jeonnam herbal farming cooperative (Hwasungun, Jeollanam-do, Korea). For supercritical co-solvent modified carbon dioxide extraction (CLS), the system and required components were acquired at 250 bar 50℃ 150 min at a flow rate of ethyl alcohol 3 mL/min for 90 min from Nano bio research center (Janseonggun, Jeollanam-do, Korea).

데이터처리

San Diego, CA, USA). Comparisons between multiple groups were performed using the one-way analysis of variance with Bonferroni post-hoc test. P-value of less than 0.

이론/모형

Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra zolium bromide (MTT) assay. Human normal keratinocytes were treated with different concentrations of C.

성능/효과

Our study shows that the expression of involucrin was increased in CLS. In conclusion, the effects of CLS treatment on skin barrier functions were evaluated. Therefore, supercritical extract from C.

참고문헌 (19)

K. Tyskiewicz, M. Konkol, E. Roj, "Supercritical Carbon Dioxide ( $scCO_2$ ) Extraction of Phenolic Compounds from Lavender (Lavandula angustifolia) Flowers: A Box-Behnken Experimental Optimization", Molecules, Vol.24, No.18. pp. 3354-3369, (2019).

상세보기
J. Liu, S. R. Farmer, "Regulating the Balance between Peroxisome Proliferatoractivated Receptor $\gamma$ and $\beta$ -Catenin Signaling during Adipogenesis", Journal of Biological Chemistry, Vol.279, No.43 pp. 45020-45027, (2004).

상세보기
G. Fruhbeck, J. Gomez-Ambrosi, F. J. Muruzabal, M. A. Burrell, "The Adipocyte: a Model for Integration of Endocrine and Metabolic Signaling in Energy Metabolism Regulation", American Journal of Physiology-Endocrinology and Metabolism, Vol.280, No.6 pp. 827-847, (2001).

상세보기
W. M. Holleran, Y. Takagi, G. K. Menon, S. M. Jackson, J. M. Lee, K. R. Feingold, P. M. Elias, "Permeability Barrier Requirements Regulate Epidermal betaglucocerebrosidase", Journal of Lipid Research, Vol.35, No.5 pp. 905-912, (1994).

상세보기
D. T. Downing, "Lipid and Protein Structures in the Permeability Barrier of Mammalian Epidermis", Journal of Lipid Research, Vol.33, No.3 pp. 301-313, (1992).

상세보기
K. T. Lee, J. Choi, W. T. Jung, J. H. Nam, H. J. Jung, H. J. Park, "Structure of a New Echinocystic acid Bisdesmoside isolated from Codonopsis lanceolata roots and the Cytotoxic Activity of Prosapogenins", Journal of Agricultural and Food Chemistry, Vol.50, No.15 pp. 4190-4193, (2002).

상세보기
S. E. Byeon, W. S. Choi, E. K. Hong, J. Lee, M. H. Rhee, H. J. Park, J. Y. Cho, "Inhibitory Effect of Saponin fraction from Codonopsis lanceolata on Immune Cell-mediated Inflammatory Responses", Archives of Pharmacal Research, Vol.32. No.6 pp. 813-822, (2009).

원문보기 상세보기
B. Kim, S. M. Lee, T. Y. Hwang, H. S. Kim, "Anti-oxidative and Skin barrier effects of Natural Plants with a Supercritical Extract", Korean Journal of Food Preservation, Vol.20, No.5 pp. 597-601, (2013).

원문보기 상세보기
B. Kim, Y. E. Choi, H. S. Kim, "Eruca sativa and its Flavonoid Components, Quercetin and Isorhamnetin, Improve Skin Barrier Function by Activation of Peroxisome proliferator-activated receptor (PPAR)- $\alpha$ and Suppression of Inflammatory Cytokines", Phytotherapy Research, Vol.28, No.9 pp. 1359-1366, (2014).

상세보기
B. Kim, H. S. Kim, "Novel Peptide Inhibits Inflammation by Suppressing of Protease activated receptor-2", European Journal of Pharmacology, Vol.832, No.1 pp. 25-32, (2018).

상세보기
K. V. Sharma, R. Sisodia, "Evaluation of the Free Radical Scavenging Activity and Radioprotective Efficacy of Grewia asiatica fruit", Journal of Radiological Protection, Vol.29, No.3 pp. 429-443, (2009).

상세보기
S. H. Lee, B. Kim, M. J. Oh, J. Y. Yoon, H. Y. Kim, K. J. Lee, J. D. Lee, K. Y. Choi, "Persicaria hydropiper (L.) spach and its Flavonoid Components, Isoquercitrin and Isorhamnetin, Activate the Wnt/- $\beta$ catenin Pathway and Inhibit Adipocyte Differentiation of 3T3-L1 cells", Phytotherapy Research, Vol.25, No.11 pp. 1629-1635, (2011).

상세보기
B. Kim, "The Activation of PPAR- $\alpha$ and Wnt/ $\beta$ -catenin by Luffa cylindrica Supercritical Carbon Dioxide Extract", Natural Product Sciences, Vol.25, No.4 pp. 341-347, (2019).

원문보기 상세보기
B. Kim, H. S. Kim, "Ricinus communis Extract Inhibits the Adipocyte Differentiation Through Activating the Wnt/ $\beta$ -catenin Signaling Pathway", Korean Jourmal of Food Preservation, Vol.24, No.4 pp. 524-528, (2017).

원문보기 상세보기
J. Y. Ahn, H. J. Lee, S. N. Kim, T. Y. Ha, "Curcumin-Induced Suppression of Adipogenic Differentiation is Accompanied by Activation of Wnt/ $\beta$ -catenin signaling", American Journal of Physiology Cell Physiolology, Vol.298, No.6 pp. 1510-1516, (2010).

상세보기
Liu J, Farmer SR, Regulating the Balance between Peroxisome Proliferator-activated receptor $\gamma$ and $\beta$ -Catenin Signaling during Adipogenesis. Journal of Biological Chemistry, 279, pp. 45020-45027, (2004).

상세보기
J. M. Jensen, R. Folster-Holst, A. Baranowsky, M. Schunck, S. Winoto-Morbach, C. Neumann, S. Schutze, E. Proksch, "Impaired Spingomyelinase Activity and Epidermal Differentiation in Atopic Dermatitis", Journal of Investigative Dermatology, Vol.122, No.6, pp. 1423-1431, (2004).

상세보기
J. H. Seo, Y. J. Lee, Y. I. Jo, J. Y. Ko. M. J. Mun, K. H. Park, S. E. Choi, "Anti-fungal, Anti-oxidant, and Antiinflammatory effects of Supercritical Fluid extracts from Ulmus davidiana", Journal of the Korea Convergence Society, Vol..9. No.8, pp. 225-233, (2018).
K. Tyskiewicz, M. Konkol, E. Roj, "The Application of Supercritical Fluid Extraction in Phenolic Compounds Isolation from Natural Plant Materials", Molecules, 23, pp. 2625-2652, (2018).

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증