[논문]증숙 더덕 용매별 추출물의 Nitric Oxide 생성 저해 효과 및 Acetylcholinesterase 저해활성

최현숙; 최두복

doi:10.9799/ksfan.2021.34.3.295

증숙 더덕 용매별 추출물의 Nitric Oxide 생성 저해 효과 및 Acetylcholinesterase 저해활성
Nitric Oxide Production and Acetylcholinesterase Inhibitory of Activity Various Extracts from Codonopsis lanceolata by Steaming Times 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.34 no.3, 2021년, pp.295 - 301

최현숙 (충청대학교 식품영양외식학부) , 최두복 (조선대학교 신산업융합학부)

Abstract ▼ AI-Helper

Steaming is a method that has traditionally been used for medicinal plant extraction. This study investigated nitrite oxide production, ferrous ion chelating activity, α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities of ethanol, acetone and hot-water extracts of Codonopsis lanceolata prepared by steaming seven times. MTT assay showed that each extract was non-toxic up to a concentration of 700 ㎍/mL confirming that there was no cytotoxicity in all extracts. The α-glucosidase, xanthine oxidase, and acetylcholinesterase inhibitory activities exhibited by the hot-water extract obtained from steaming seven times were higher (83.1%) than the other extracts. Higher production of nitrite oxide and better ferrous chelating activity was recorded with hot-water extract compared to ethanol and acetone extracts. These results indicated that more steaming of Codonopsis lanceolata extracts would be required to validate the possibility of developing antioxidants. Also, further study is needed to determine if the components present in the tested extracts might be useful in the prevention of Alzheimer's disease. These results showed that hot-water extracts may be useful for their antioxidant and the production inhibitory activity of nitrite oxide. It will be helpful in the investigation of the constituent analysis of the steam-processed product of Codonopsis lanceolata.

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표/그림 (5)

표 Table 1. Sample code name of the Codonopsis lanceolata extracts
그림 Fig. 1. Effect of the ethanol (EDS7), acetone (ADS7), hotwater (HDS7) extracts of 7 Steamed Codonopsis lanceolata on the cell viability of RAW264.7 cells. The viability of cells was measured with MTT assay. Results were expressed as % of control absorbance. Results are mean±S.D. of triplicate data.
그림 Fig. 2. Reducing activity of LPS-induced NO production in RAW264.7 cells and Fe²⁺ chelating activity of the ethanol (EDS7), acetone (ADS7), hot-water (HDS7) extracts of 7 steamed Codonopsis lanceolata. Results are mean±S.D. of triplicate data. Different letters are significantly different at p<0.05 by Duncan's multiple range test.
그림 Fig. 3. α-Glucosidase and Xanthine oxidase inhibitory activities of the ethanol (EDS7), acetone (ADS7), hot-water (HDS7) extracts of 7 steamed Codonopsis lanceolata. Results are mean±S.D. of triplicate data. Different letters are significantly different at p<0.05 by Duncan's multiple range test.
그림 Fig. 4. Inhibitory effects against AChE of the ethanol (EDS7), acetone (ADS7), hot-water (HDS7) extracts of 7 Steamed Codonopsis lanceolata. Results are mean±S.D. of triplicate data. Different letters are significantly different at p<0.05 by Duncan’s multiple range test.

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