[논문]Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal

Son, Se Hyun; Seo, Kwang Won; Kim, Yeong Bin; Noh, Eun Bi; Lee, Keun-Woo; Oh, Tae-Ho; Kim, Seung-Joon; Song, Jae-Chan; Kim, Tae-Wan; Lee, Young Ju

doi:10.14405/kjvr.2020.60.3.173

[국내논문] Genotypic characterization of fluoroquinolone-resistant Escherichia coli isolates from edible offal 원문보기

Korean journal of veterinary research = 대한수의학회지, v.60 no.3, 2020년, pp.173 - 177

Son, Se Hyun (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Seo, Kwang Won (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Kim, Yeong Bin (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Noh, Eun Bi (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Lee, Keun-Woo (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Oh, Tae-Ho (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Kim, Seung-Joon (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Song, Jae-Chan (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Kim, Tae-Wan (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University) , Lee, Young Ju (College of Veterinary Medicine & Zoonoses Research Institute, Kyungpook National University)

Abstract ▼ AI-Helper

Edible offal is easily contaminated by Escherichia coli (E. coli) and fluoroquinolone (FQ)-resistant E. coli is considered a serious public health problem, thus, this study investigated the genetic characteristics of FQ-resistant E. coli from edible offal. A total of 22 FQ-resistant E. coli isolates were tested. A double mutation in each gyrA and parC led the highest MIC. Four (18.2%) isolates carried plasmid-mediated quinolone resistance genes. The fimH, eaeA, escV, astA, and iucC genes were confirmed. Seventeen isolates (77.3%) were positive for plasmid replicons. The isolates showed high genetic heterogeneity based on pulsed-field gel electrophoresis patterns.

Keyword

표/그림 (3)

그림 Fig. 1. Antimicrobial resistance patterns in 22 fluoroquinolone-resistant Escherichia coli isolates from edible offal. AM, ampicillin; AMC, amoxicillin-clavulanic acid; CZ, cefazolin; CF, cephalothin; CL, cephalexin; FOX, cefoxitin; CXM, cefuroxime; CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; SXT, trimethoprim-sulfamethoxazole; NA, nalidixic acid; IPM, imipenem; GM, gentamicin; C, chloramphenicol; TE, tetracycline.
표 Table 1. Distribution of multidrug resistance pattern among 22 fluoroquinolone-resistant Escherichia coli isolates from edible offal
그림 Fig. 2. Genetic characteristics of 22 fluoroquinolone-resistant Escherichia coli isolates from edible offal. (A) Pulsed-field gel electrophoresis

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문제 정의

coli in livestock and meat products have been conducted [3-5], their signifi-cance in edible offal has not been satisfactorily explored. The objective of the current study is to investigate genetic characteristics of FQ-resistant E. coli isolates from edible offal.

대상 데이터

One hundred-eighteen E. coli isolates were collected from edible offal sam-ples (the heart, liver, stomach or gizzard, small intestine, and large intestine) produced at 8 chicken, 9 pig, and 7 cattle slaughterhouses located in the cen-tral and southern regions of Korea from January to October 2017. The method for isolating E.

이론/모형

Pulsed-field gel electrophoresis (PFGE) of the FQ-resis-tant E. coli isolates was performed in accordance with CDC PulseNet protocol [12], using a CHEF-Mapper apparatus (Bio-Rad Laboratories, USA). The dendrogram of PFGE pat-terns was constructed via Dice coefficients and the unweighted pair group method with arithmetic mean (UPGMA).
coli isolates was performed in accordance with CDC PulseNet protocol [12], using a CHEF-Mapper apparatus (Bio-Rad Laboratories, USA). The dendrogram of PFGE pat-terns was constructed via Dice coefficients and the unweighted pair group method with arithmetic mean (UPGMA).

성능/효과

In conclusion, this study provides evidence for the role of edible offal in the dissemination of FQ-resistance in humans via the food chain and shows importance of enhancing hygiene to reduce the cross-contamination in the production of edible offal.

참고문헌 (15)

Redgrave LS, Sutton SB, Webber MA, Piddock LJ. Fluoroquinolone resistance: mechanisms, impact on bacteria, and role in evolutionary success. Trends Microbiol 2014;22:438-445.

상세보기
Saide-Albornoz JJ, Knipe CL, Murano EA, Beran GW. Contamination of pork carcasses during slaughter, fabrication, and chilled storage. J Food Prot 1995;58:993-997.

상세보기
Hu YS, Shin S, Park YH, Park KT. Prevalence and mechanism of fluoroquinolone resistance in Escherichia coli isolated from swine feces in Korea. J Food Prot 2017;80:1145-1151.

상세보기
Koo HJ, Woo GJ. Characterization of antimicrobial resistance of Escherichia coli recovered from foods of animal and fish origin in Korea. J Food Prot 2012;75:966-972.

상세보기
Oh JY, Kwon YK, Tamang MD, Jang HK, Jeong OM, Lee HS, Kang MS. Plasmid-mediated quinolone resistance in Escherichia coli isolates from wild birds and chickens in South Korea. Microb Drug Resist 2016;22:69-79.

상세보기
Son SH, Seo KW, Kim YB, Jeon HY, Noh EB, Lee YJ. Molecular characterization of multidrug-resistant Escherichia coli isolates from edible offal in Korea. J Food Prot 2019;82:1183-1190.

상세보기
Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twentythird Informational Supplement. CLSI document M100-S23. Wayne: Clinical and Laboratory Standards Institute; 2013.
Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, Paterson DL, Rice LB, Stelling J, Struelens MJ, Vatopoulos A, Weber JT, Monnet DL. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012;18:268-281.

상세보기
Kagambega A, Martikainen O, Lienemann T, Siitonen A, Traor AS, Barro N, Haukka K. Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso. Int J Food Microbiol 2012;153:154-158.

상세보기
Tarchouna M, Ferjani A, Ben-Selma W, Boukadida J. Distribution of uropathogenic virulence genes in Escherichia coli isolated from patients with urinary tract infection. Int J Infect Dis 2013;17:e450-e453.

상세보기
Johnson TJ, Wannemuehler YM, Johnson SJ, Logue CM, White DG, Doetkott C, Nolan LK. Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Appl Environ Microbiol 2007;73:1976-1983.

상세보기
Ribot EM, Fair MA, Gautom R, Cameron DN, Hunter SB, Swaminathan B, Barrett TJ. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis 2006;3:59-67.

상세보기
Animal and Plant Quarantine Agency (APQA). National Antimicrobial Resistance Monitoring Program. Available from: https://ebook.qia.go.kr/20180704_110723.
Moon DC, Seol SY, Gurung M, Jin JS, Choi CH, Kim J, Lee YC, Cho DT, Lee JC. Emergence of a new mutation and its accumulation in the topoisomerase IV gene confers high levels of resistance to fluoroquinolones in Escherichia coli isolates. Int J Antimicrob Agents 2010;35:76-79.

상세보기
Yang QE, Sun J, Li L, Deng H, Liu BT, Fang LX, Liao XP, Liu YH. IncF plasmid diversity in multi-drug resistant Escherichia coli strains from animals in China. Front Microbiol 2015;6:964.

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