[논문]In vivo Genotoxicity Assessment of Matrine and the Water Extract of Sophorae Radix Using a Comet Assay

Heo, Seongmoo; Lee, Jiyeon; Jeon, Hyoungwoon; Kim, Myoungjun; Chung, Young-Shin

doi:10.13103/jfhs.2021.36.2.118

[국내논문] In vivo Genotoxicity Assessment of Matrine and the Water Extract of Sophorae Radix Using a Comet Assay 원문보기

한국식품위생안전성학회지 = Journal of food hygiene and safety, v.36 no.2, 2021년, pp.118 - 123

Heo, Seongmoo (Department of Biotechnology and The Research Institute for Basic Sciences, Hoseo University) , Lee, Jiyeon (Department of Biotechnology and The Research Institute for Basic Sciences, Hoseo University) , Jeon, Hyoungwoon (Department of Biotechnology and The Research Institute for Basic Sciences, Hoseo University) , Kim, Myoungjun (Department of Biotechnology and The Research Institute for Basic Sciences, Hoseo University) , Chung, Young-Shin (Department of Biotechnology and The Research Institute for Basic Sciences, Hoseo University)

초록
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본 연구는 고삼 물추출물과 주 성분인 마트린의 유전독성 유발능을 마우스 간세포 및 혈구세포에서 체내 단세포전기영동 시험법을 사용하여 평가하였다. OECD TG 489에서 정하고 있는 체내 알카리코멧 시험법(in vivo alkanine Comet assay)을 수컷 마우스를 사용하여 수행하였다. 고삼물추출물의 투여 용량은 한계 용량을 포함하도록 체중 대비 1,000와 2,000 mg/kg으로 하였다. 마트린의 세 투여용량은 50, 100, 200 mg/kg으로 정하였고, 최고 투여용량은 용량 설정시험을 통하여 설정하였다. 음성대조물질과 시험물질은 경구로 3회 투여하였고, 양성대조물질(ethyl methanesulfonate, EMS)은 2회 투여하였다. 마지막 투여 후 3-4시간 내에 간조직과 혈액을 채취하였고 단세포전기영동을 알카리 조건에서 수행하였다. 코멧 시험 결과, 고삼 물추출물과 마트린에 의한 체중의 변화가 관찰되지 않았으며, 간세포와 혈구세포에서 통계적으로 유의성 있는 유전자 손상을 유발하지 않았다. 따라서, 본 연구조건에서 고삼 물추출물과 마트린은 유전독성 유발능이 없는 것으로 사료된다.

Abstract ▼ AI-Helper

The genotoxic potentials of the water extract of Sophorae Radix (WSR) and matrine as a major compound were investigated in murine hepatocytes and blood cells using single-cell gel electrophoresis. According to the test guidelines of OECD TG 489, an in vivo alkaline Comet assay was performed using male mice. The treatment doses of the WSR were 1,000 and 2,000 mg/kg body weight to include the limiting dose. The three test doses for matrine were 50, 100, and 200 mg/kg, and the maximum dose was estimated from a dose range-finding test. The negative control and test articles were administered three times by oral gavage, and the positive control (ethyl methanesulfonate, EMS) was administered twice. Within 3-4 h after the last treatment, the liver tissue and blood were collected, and single-cell electrophoresis was performed under alkaline conditions. The in vivo Comet assay showed no changes in body weights during the treatments with the test articles. The WSR and matrine did not induce significant DNA damage in the liver and blood cells of mice compared to the vehicle treatment. In conclusion, matrine and the WSR may not have genotoxic potential.

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참고문헌 (43)

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