[논문]Two Unrecoreded Species Belonging to Penicillium Section Exilicaulis in South Korea

Park, Myung Soo; Kim, Sung Hyun; Lee, Jun Won; Kim, Ji Seon; Cho, Yoonhee; Lim, Young Woon

doi:10.4489/kjm.20200019

[국내논문] Two Unrecoreded Species Belonging to Penicillium Section Exilicaulis in South Korea 원문보기

한국균학회지 = The Korean journal of mycology, v.48 no.3, 2020년, pp.175 - 185

Park, Myung Soo (School of Biological Sciences and Institute of Microbiology, Seoul National University) , Kim, Sung Hyun (School of Biological Sciences and Institute of Microbiology, Seoul National University) , Lee, Jun Won (School of Biological Sciences and Institute of Microbiology, Seoul National University) , Kim, Ji Seon (School of Biological Sciences and Institute of Microbiology, Seoul National University) , Cho, Yoonhee (School of Biological Sciences and Institute of Microbiology, Seoul National University) , Lim, Young Woon (School of Biological Sciences and Institute of Microbiology, Seoul National University)

Abstract ▼ AI-Helper

Penicillium in section Exilicaulis is characterized by non-vesiculate monoverticillate and biverticillate stipes. Species in sect. Exilicaulis are commonly found in soil and plants in terrestrial environments; however, only a few species have been reported in Korea. To investigate the diversity of Penicillium sect. Exilicaulis, Penicillium species were isolated from terrestrial and marine environments. Based on sequence analyses of β-tubulin, calmodulin, and the second largest subunit of RNA polymerase II loci, 19 strains of Penicillium in sect. Exilicaulis were identified as P. citreonigrum, P. citreosulfuratum, P. corylophilum, P. menonorum, P. rubefaciens, P. velutinum, Penicillium sp. 1, and Penicillium sp. 2. Two of them, P. citreonigrum and P. citreosulfuratum, were confirmed to be new to Korea. Molecular phylogenies and detailed descriptions of the two unrecorded species are provided.

주제어

표/그림 (4)

표 Table 1. Summary and GenBank accession numbers for Penicillium in section Exilicaulis.
그림 Fig. 1. Maximum likelihood phylogenetic tree of the combined data set of BenA, CaM, and RPB2 gene sequences used to identify strains to the species level in Penicillium section Exilicaulis. Bootstrap scores of >70 are presented at the nodes. The scale bar indicates the number of nucleotide substitutions per site. "T" indicates the ex-type strains. Strains reported in the current study are represented in bold. The species labeled in blue represent previously unrecorded species in South Korea. The names in red are potential new species.
그림 Fig. 2. Penicillium citreonigrum SFCP0024 in 7-day-old cultures at 25℃. (A-C) Colonies grown on Czapek yeast autolysate agar (CYA), malt extract agar (MEA), and yeast extract sucrose agar (YES) from left to right (top=obverse, bottom=reverse). (D-F) Conidiophores; (G) Conidia (scale bar: D-G=10 μm).
그림 Fig. 3. Penicillium citreosulfuratum SFC20170821-M07 in 7-day-old cultures at 25℃. (A-C) Colonies grown on Czapek yeast autolysate agar (CYA), malt extract agar (MEA), and yeast extract sucrose agar (YES) from left to right (top=obverse, bottom=reverse). (D-F) Conidiophores; (G) Conidia (scale bar: D-G=10 μm).

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가설 설정

Molecular identification was performed in two steps. First, we identified strains belonging to section Exilicaulis by comparison to the BenA sequences of type strains. Next, each strain was identified to the species level by analyzing the combined dataset of the three loci (BenA, CaM, and RPB2).

제안 방법

The PCR products were purified using the Expin^TM PCR Purification Kit (GeneAll Biotechnology, Seoul, Korea), according to the manufacturer's instructions. DNA sequencing was performed on an ABI Prism 3700 genetic analyzer (Life Technologies, Gaithersburg, MD, USA) at Macrogen (Seoul, Korea) using the PCR primers.
First, we identified strains belonging to section Exilicaulis by comparison to the BenA sequences of type strains. Next, each strain was identified to the species level by analyzing the combined dataset of the three loci (BenA, CaM, and RPB2). Penicillium trzebinskii CBS 351.
The Methuen Handbook of Color was used for the color names and alphanumeric codes for macromorphological characteristics [27]. The microscopic features were observed under a light microscope (Eclipse 80i, Nikon, Tokyo, Japan) using colonies grown on MEA at 25℃ for seven days.

대상 데이터

A total of 19 Penicillium strains were identified in this study. Twelve strains were isolated from egg masses of Arctoscopus japonicas (2 strains), mudflats (4), sea sand (5), and seaweed (1) using previously described methods [18-20].
7; bootstrap support=100%). SFC20200821-M04 and SFC20200821-M05 grouped with P. menonorum NRRL 50410 (type strain) (sequence similarity for BenA=100% and CaM=99.8- 100%, RPB2=99.7; bootstrap support=100%). SFC20140101-M799 formed a monophyletic group with P.
5%, RPB2=100; bootstrap support=99%). Three strains grouped with P. velutinum NRRL 2069 (type strain) (sequence similarity for BenA=100%, RPB2=99.9; bootstrap support=100%). The remaining two groups formed distinct group with previously reported species.

성능/효과

8%). Based on these results, P. rubidurum KNU14-12 might be a new species rather than P. rubidurum. Penicillium decumbens and P.
Nine species in section Exilicaulis have been previously reported in Korea [31]. Five species of them were not found in this study: P. decumbens, P. erubescens, P. melinii, P. restrictum and P. rubidurum. Two species (P.
Five strains formed a monophyletic group with P. citreonigrum NRRL 761 (type strain), CBS 321.59, and NRRL 1187 (sequence similarity for BenA=98.8-100%, CaM=98.0-100%, and RPB2=98.5-99.2%; bootstrap support=89%). Three strains grouped with P.
KP055596) [14]. However, P. rubidurum KNU14-12 formed a phylogenetically distinct group with the type strain (CBS 609.73) of P. rubidurum and showed the highest similarity of ITS sequence with P. parvum CBS 570.73 (sequence similarity for ITS=99.8%). Based on these results, P.
2%; bootstrap support=89%). Three strains grouped with P. citreosulfuratum DTO 290-I4 (type strain), CV 2015, and NRRL 31271 (sequence similarity for BenA=100% and CaM=99.8-100%, RPB2=99.9; bootstrap support=100%). Two strains formed a monophyletic group with the type strain (CBS 312.
9; bootstrap support=100%). Two strains formed a monophyletic group with the type strain (CBS 312.48) of P. corylophilum, CBS 231.38 and CBS127808 (sequence similarity for BenA=98.8-99.0%, CaM=99.8%, RPB2=99.7; bootstrap support=100%). SFC20200821-M04 and SFC20200821-M05 grouped with P.

후속연구

due to the minor morphological differences. Additional examination for a more detailed morphological comparison with phylogenetically similar species will be required to identify these species.

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