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NTIS 바로가기생태와 환경 = Korean journal of ecology and environment, v.53 no.3, 2020년, pp.265 - 274
박재원 (전남대학교 해양융합과학과) , 박기연 (전남대학교 수산과학연구소) , 곽인실 (전남대학교 해양융합과학과)
Silver croaker (Pennahia argentata) is a turbulent species that is widely distributed worldwide and is mainly found in the bottom of the ocean. In the study, we characterized the cytochrome c oxidase subunit I (COI) gene of the mitochondrial DNA (mtDNA) on P. argentata inhabiting Gwangyang Bay and a...
핵심어 | 질문 | 논문에서 추출한 답변 |
---|---|---|
보구치란 무엇인가? | 보구치(Pennahia argentata)는 농어목 민어과에 속하는 난류성 어종으로 민어과 물고기 중 개체수가 많은 종이다(Koh et al., 2014). 암수에 따른 보구치의 비율은 53:47로 암컷 비율이 수컷보다 높게 나타났으며 크기는 평균 전장(TL)이 암컷은 24. | |
보구치의 주 먹이원은 무엇인가? | , 2020). 보구치의 주 먹이원은 새우류로 먹이원 분석 연구에서 153개체 보구치 위(gut)에서 발견된 217개체의 먹이생물 중 가장 주요한 먹이 생물이 새우류(Macrura)로 출현 빈도는 68.6%를 차지하였고, 개체수 비 66.4%, 습중량 비는 43. | |
보구치 total genomic DNA 추출 방법은 무엇인가? | 보구치 total genomic DNA 추출은 Bioneer의 AccuPrep genomic DNA extraction kit를 사용하여 제공되는 Protocol에 따라 수행하였다. 실험 과정은 EP tube에 TL buffer 200μL를 넣고 근육조직을 균질하게 homogenize하고 Protei-nase K 20 μL, RNase A 10 μL를 넣어 단백질과 RNA를 제거하였다. 60°C에서 1~2시간 Lysis 과정을 거치고 GB buffer와 Ethanol을 넣고 원심분리 후 상등액을 취하였다. WA1과 WA2 buffer를 통해 washing 과정을 거친 후 EA buffer로 Genomic DNA를 elution하여 사용시까지 -80°C Deep freezer(Thermo Fisher Scientific)에 보관하였으며 각지점 위치, 종명, 추출한 날짜 등의 정보를 기록해 두었다. |
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