[논문]복합 생균제가 육계의 생산성, 육질, 지방산 조성 및 면역 반응에 미치는 영향

시딕 샤리프 하산; 황채연; 최호성; 심관섭

doi:10.11625/kjoa.2020.28.3.431

복합 생균제가 육계의 생산성, 육질, 지방산 조성 및 면역 반응에 미치는 영향
Effects of Complex Probiotics on Productivity Index, Fatty Acid Composition and Immune Response in Broilers 원문보기

韓國有機農業學會誌 = Korean journal of organic agriculture, v.28 no.3, 2020년, pp.431 - 447

시딕 샤리프 하산 (전북대학교 축산학과) , 황채연 (전북대학교 축산학과) , 최호성 (전북대학교 동물생명공학과) , 심관섭 (전북대학교 동물생명공학과)

Abstract ▼ AI-Helper

This study was conducted to investigate the efficacy of mixed probiotic on the immunity, productivity index and mortality rate in the broiler. Total of 120 one-day-old Ross broilers chicks were randomly assigned into two treatments (control dietary group and probiotic-treated group) with three replications of each treatment. The probiotic group broiler had a lower mortality rate than control during the experimental period. The productivity index in the probiotic group increased significantly than the control group. The weight of the bursa of fabricius was high in the probiotic-treated group than the control group. Activated the immunity level after fed the probiotic mixed diet compared to the control group. Furthermore, the probiotic diet significantly decreased the saturated fatty the control group. Whereas the probiotic mixed diet increased the unsaturated fatty acid than the control group. Afterward, the diet including probiotic induced positive impact on broilers immunity level. This indicates that a probiotic mixed diet could be protecting the intestine from the invasion of a pathogenic organism. It would be beneficial to the poultry industries by decrease the broiler mortality rate with elevated the immunity.

주제어

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문제 정의

Considering the beneficial effects of using mixed probiotics, the aim of our study was to evaluate the effect of mixed probiotics (Bacillus subtilis, Lactobacillus plantarum and Saccharomyces cerevisiae) on the broiler.
, 2017). This study revealed this probiotic may have antiinflammatory effects and as well as prevent the inflammatory action in the intestine.

가설 설정

In this study, we were assessed the probiotic supplemented feed will increase the primary immune organ. Our result showed that this experimental probiotic increased only the weight of bursa of fabricius, but this probiotic did not affect other organs, maybe this probiotic is not related to body weight gain.

이론/모형

After reaching the desired temperature, samples were taken out and cooled at room temperature. From the core of the muscle, 1.27 cm diameter myofiber collect and used Digital Meat Tenderness Meter of Model C-LM3 (Northeast Agricultural University, Harbin, China) for Shear force measure. During the experiment test speed was 5 mm/s.
Real-time PCR was performed by using the SYBER® Green Real-time PCR Master Mix (TOYOBO).
This study follows the previous method for calculation of productivity index (PI) (Martins et al., 2016). It was calculated from broiler growth performance and mortality rate.
The thermal cycling temperature and duration were 95℃ for 30 s, 95℃ for 5 s and 59℃ for 5 s finally followed by 40 cycles. To determine the relative fold-changes and all data were normalized with the housekeeping gene GAPDH by the used 2∆∆CT method. The result of the qPCR was calculated by ∆Ct value (Ct gene of interest - Ct housekeeping gene).

성능/효과

In conclusion, this experiment indicates that probiotic supplement had remarkable positive impact on the mortality and productivity index during the experimental period. The weight of bursa of fabricius (primary lymphoid organ) increased for using probiotics, as well as this probiotic increase the immunity in broiler.

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표제어: PCR

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