[논문]생물전환에 의해 생성된 Compound K의 항염증 및 독성 효과

김무성; 신현영; 김현경; 강지성; 정경환; 유광원; 문기성; 이향렬

doi:10.12925/jkocs.2021.38.6.1466

생물전환에 의해 생성된 Compound K의 항염증 및 독성 효과
Anti-inflammatory activity and toxicity of the compound K produced by bioconversion 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.38 no.6, 2021년, pp.1466 - 1475

김무성 (마크로케어) , 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템융합전공) , 김현경 (한국교통대학교 식품생명학부) , 강지성 (한국교통대학교 식품생명학부) , 정경환 (한국교통대학교 식품생명학부) , 유광원 (한국교통대학교 식품생명학부) , 문기성 (한국교통대학교 식품생명학부) , 이향렬 (한국교통대학교 식품생명학부)

초록
AI-Helper

Compound K (20-O-β-(_D-glucopyranosyl)-20(S)-protopanaxadiol)는 진세노사이드의 활성성분이다. Compound K는 경구 투여 후 Rb₁, Rb₂ 및 Rc가 사람의 장내 미생물의 β-glucosidase에 의해 생물전환 과정을 거쳐 생성된다고 알려져 있다. 본 연구는 생물전환된 인삼농축액에서 얻은 compound K를 이용해 항염증 및 독성을 조사하였다. 세포독성평가 결과, compound K는 0.001~1 ㎍/mL의 농도범위에서 유의적인 세포독성은 나타나지 않았으며, LPS로 염증이 유발된 RAW 264.7 세포에서 TNF-α, MCP-1, IL-6 및 NO의 생성을 억제하는 것으로 확인되었다. 동일 농도범위에서 TNF-α 및 IFN-γ로 염증이 유발된 HaCaT 세포는 compound K의 처리로 인해 IL-8의 생성을 감소시키는 것으로 나타났지만, IL-6의 경우 일부 농도에서 생성을 감소시켰으나, 통계적인 유의성은 나타나지 않았다. Brine shrimp를 이용한 치사율 검정법에서 compound K의 LC₅₀는 0.37mg/mL로 다소의 독성을 함유하고 있는 것으로 나타났으나 compound K가 35% 고함유된 생물전환물은 LC₅₀가 0.87mg/mL로 나타나 상대적으로 낮은 독성을 보였다. 따라서 이 생성물은 향후 여드름 완화용 화장품 개발에 사용할 수 있는 매우 우수한 기능성 소재가 될 수 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Compound K (20-O-β-(D-glucopyranosyl)-20(S)-protopanaxadiol) is an active ingredient of ginsenosides. Compound K has been known to produce from biotransformation by β-glucosidase action of human intestinal microbes after oral admistration of ginseng. We have investigated the cytotoxicity of compound K obtained from bio-converted ginseng extract. As a result, compound K showed no significant cytotoxicity in the concentration of 0.001 to 1 ㎍/mL and inhibited the production of TNF-α, MCP-1, IL-6 and NO in RAW 264.7 cells induced by LPS inflamation. In the same concentration, HaCaT cells induced by inflammation with TNF-α and IFN-γ decreased IL-8 production due to compound K treatment. In the brine shrimp lethality assay, the LC50 of compound K was 0.37 mg/mL indicating some toxicity, but the bioconverted product containing 35% compound K showed relatively low toxicity with an LC50 of 0.87 mg/mL. These results suggest that the compound K enriched extract is a potential functional material for acne relief cosmetic products.

주제어

표/그림 (4)

그림 Fig. 1. Cytotoxic effect of compound K using (A) RAW 264.7 macrophage and (B) HaCaT keratinocyte. Each sample were treated to RAW 264.7 or HaCaT cells, and inflammation inducer, such as lipopolysaccharide (LPS) or TNF-α and IFN-Υ (T+I) were subsequently stimulated for 24 hr. A dexamethasone (50 and 20 μg/mL, for RAW 264.7 and HaCaT, respectively) was used as positive control. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between inflammation group (LPS or T+I) and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 2. Anti-inflammatory activities of compound K on LPS-stimulated RAW 264.7 cell. RAW 264.7 cells were treated with samples followed by LPS treatment for 24 hr, and production of pro-inflammatory mediators such as (A) TNF-α, (B) MCP-1, (C) IL-6 and (D) nitric oxide (NO) were estimated. Only medium was used as negative control (NC). A dexamethasone (50 μg/mL) or N-nitroarginine methyl ester (L-NAME; 50 μg/mL) were used as positive control (PC) for TNF-α, MCP-1 and IL-6, or NO experiments, respectively. Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between LPS-treated group (LPS) and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 3. Anti-inflammatory activities of compound K on TNF-α+IFN-γ (T+I)-stimulated HaCaT keratinocyte. HaCaT keratinocyte were treated with each sample followed by TNF-α+IFN-γ (T+I) treatment for 24 hr, and the production of pro-inflammatory mediators such as (A) IL-6 and (B) IL-8 were estimated. Only medium was used as negative control (NC). A dexamethasone (20 μg/mL) were used as positive control (PC). Results are expressed as mean±S.D. of three independent test in triplicate. Asterisks mean significant difference between T+I-treated group (T+I) and each group by Student’s t-test. *p<0.05, **p<0.01, ***p<0.001.
그림 Fig. 4. Cytotoxic effects of the red ginseng extract(A), compound K (B) and 35% compound K enriched extract(C) on brine shrimp larvae for an incubation period of 24hr. Results are expressed as mean of three independent test in triplicate.

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상세보기
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