[논문]뇌전증 동물 모델에 대한 백출 추출물의 보호 효과

강소희; 이수은; 이아영; 서윤수; 문창종; 김성호; 이지혜; 김중선

doi:10.6116/kjh.2021.36.6.1.

뇌전증 동물 모델에 대한 백출 추출물의 보호 효과
Protective effects of Atractylodis Rhizoma Alba Extract on seizures mice model 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.36 no.6, 2021년, pp.1 - 8

강소희 (전남대학교 수의과대학) , 이수은 (한국한의학연구원 한약자원연구센터) , 이아영 (한국한의학연구원 한약자원연구센터) , 서윤수 (한국한의학연구원 한약자원연구센터) , 문창종 (전남대학교 수의과대학) , 김성호 (전남대학교 수의과대학) , 이지혜 (세명대학교 한의과대학) , 김중선 (전남대학교 수의과대학)

Abstract ▼ AI-Helper

Objectives : Atractylodis rhizoma Alba has been traditionally used as a medicinal resource that is used for enhancing Qi (氣) in traditional medicine in Korea, China, and Japan. This study investigated the protective effects of Atractylodis rhizoma Alba extract (ARE) against trimethyltin (TMT), a neurotoxin that causes selective hippocampal injury, using both in vitro and in vivo models. Methods : We investigated the effects of ARE on TMT- (5mM) induced cytotoxicity in primary cultures of mouse hippocampal cells (7 days in vitro ) and on hippocampal injury in C57BL/6 mice injected with TMT (2.6 mg/kg). Results : We observed that ARE treatment (0 - 50 ㎍/mL) significantly reduced TMT-induced cytotoxicity in cultured hippocampal neurons in a dose-dependent manner, based on results of lactate dehydrogenase and 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assays. Additionally, this study showed that orally administered ARE (5 mg/kg; between -6 and 0 days before TMT injection) significantly attenuated seizures in adult mice. Furthermore, quantitative analysis of allograft inflammatory factor-1 (Iba-1)- and glial fibrillary acidic protein (GFAP)- positive cells showed significantly reduced levels of Iba-1- and GFAP-positive cell bodies in the dentate gyrus of mice treated with ARE prior to TMT injection. These findings indicate the significant protective effects of ARE against the TMT-induced massive activation of microglia and astrocytes in the hippocampus. Conclusions : We conclude that ARE minimizes the detrimental effects of TMT-induced hippocampal neurotoxicity, both in vitro and in vivo . Our findings may serve as useful guidelines to support ARE administration as a promising pharmacotherapeutic approach to hippocampal degeneration.

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표/그림 (5)

그림 Fig. 1. Main target and efficacy in Traditional Korean Medicine correlation network of Atractylodis Rhizoma Alba using Oriental Medicine Advanced Searching Integrated System (OASIS).
그림 Fig. 2. Protective effects of Atractylodis Rhizoma Alba extract (ARE) on trimethyltin (TMT)- induced cytotoxicity. ARE treatment reduced cytotoxic effects of TMT on hippocampal neurons. (A): Lactate dehydrogenase (LDH) assay. (B): 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Values are reported as mean ± SE, ^*p < 0.05, ^***p < 0.01 vs. vehicle-treated controls. ^#p < 0.05 vs. TMT-treated controls.
그림 Fig. 3. Protective effects of ARE on seizure symptoms in TMT-treated mice. (A): Schematic diagram of drug treatment, tissue preparation, and behavioral test. (B): ARE treatment ameliorated TMT-induced seizure behaviors. Values are reported as mean ± SE, ^#p < 0.05 vs. TMT-treated time-matched controls.
그림 Fig. 4. Protective effects of ARE on astrocyte levels in the dentate gyrus (DG), 4 days after TMT treatment. (A): Representative images (X 200) showing GFAP immunostaining in untreated control, TMT control, and ARE 25 + TMT group. Black line area represents DG and black dotted area represents DG hilus. Scale bar = 500 ㎛. (B): The graphs depict the relative number of GFAP-positive cells per DG and DG hilus in the hippocampus sections. Values are reported as mean ± SE, *p < 0.05, **p < 0.01 vs. vehicle-treated controls. ^#p < 0.05 vs. TMT-treated time-matched controls.
그림 Fig. 5. Protective effects of ARE on microglial cell activation in the hippocampus 4 days after TMT treatment. (A): Representative images (X 200) showing Iba-1 immunostaining in untreated control, TMT control, and ARE 25 + TMT group. black line area represents DG and black dotted area represents DG hilus. Scale bar = 500 ㎛. (B): The graphs depict the relative number of Iba-1-positive cells per DG and DG hilus in the hippocampus sections. Values are reported as mean ± SE, *p < 0.05, **p < 0.01 vs. vehicle-treated controls. ^#p < 0.05 vs. TMT-treated time-matched controls.

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