[논문]더덕, 도라지 표고균사발효물의 유용성분 및 생리활성

고영우; 윤경원; 김경제; 진성우; 임승빈; 하늘이; 정희경; 김승주; 김복선; 최유진; 송다혜; 서경순

doi:10.14480/jm.2021.19.4.300

더덕, 도라지 표고균사발효물의 유용성분 및 생리활성
Beneficial constituents and physiological activity of fermented Codonopsis lanceolata and Platycodon grandiflorus by Lentinula edodes mycelium 원문보기

Journal of mushrooms = 한국버섯학회지, v.19 no.4, 2021년, pp.300 - 309

고영우 ((재)장흥군버섯산업연구원) , 윤경원 (순천대학교 바이오한약자원학과) , 김경제 ((재)장흥군버섯산업연구원) , 진성우 ((재)장흥군버섯산업연구원) , 임승빈 ((재)장흥군버섯산업연구원) , 하늘이 ((재)장흥군버섯산업연구원) , 정희경 ((재)장흥군버섯산업연구원) , 김승주 (데이앤바이오(주)농업회사법인) , 김복선 (데이앤바이오(주)농업회사법인) , 최유진 ((재)임실치즈앤식품연구소) , 송다혜 ((재)임실치즈앤식품연구소) , 서경순 ((재)장흥군버섯산업연구원)

초록
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한약재로 사용되는 더덕과 도라지에 표고의 유용성분을 함유한 소재를 개발하고자 더덕표고균사발효물(FCLM)과 더덕도라지균사발효물(FPLM)을 제조하였다. 두 가지 발효물의 일반성분, 유리당, 유기산, 𝛽-glucan, ergothioneine, ergosterol, vitamin D₂ 함량 및 3T3-L1 지방전구세포의 분화에 미치는 영향을 연구하였다. 일반성분 분석결과, FCLM에서 조섬유와 조지방함량이 FPLM에 비하여 높게 나타났으며, FPLM은 조단백질과 가용성무질소물의 함량이 FCLM에 비하여 높게 나타났다. 유리당은 FCLM과 FPLM 모두 arabinose, glucose, sucrose 3종이 검출되었으며, 총 유리당 함량은 FPLM에서 높게 나타났다. 유기산 함량은 발효 전 더덕과 도라지에 비하여 발효 후 FCLM과 FPLM에서 낮게 나타났다. 𝛽-Glucan 함량은 FCLM과 FPLM 두 발효물에서 대조구로 사용한 표고보다 높은 함량을 보였다. 항산화 물질로 알려진 ergothioneine의 함량은 FCLM이 FPLM보다 높게 나타났다. Ergosterol 함량은 대조구로 사용한 표고에서 가장 높게 나타났으며, 발효물들은 비슷한 함량을 나타내었다. Vitamin D₂는 발효물들에서만 검출되었으며, FPLM (0.58±0.01 mg%)가 FCLM (0.47±0.01 mg%)보다 높은 함량을 보였다. 더덕과 도라지 표고균사발효물인 FCLM과 FPLM는 3T3-L1 지방전구세포에 대하여 발효 전 더덕과 도라지에 비하여 높은 수준의 세포 생존율을 나타내었다. 또한 FCLM과 FPLM은 3T3-L1 지방전구세포 분화를 발효 전 더덕과 도라지에 비하여 높은 수준으로 억제하여, 비만 억제 효과가 있는 것으로 기대된다.

Abstract ▼ AI-Helper

This study was conducted to develop food and medicinal products containing useful components of Lentinula edodes in Codonopsis lanceolata and Platycodon grandiflorus for use as herbal medicine. We manufactured C. lanceolata (FCLM) and P. grandiflorus (FPLM) extract fermented with L. edodes mycelium. The effect of the two fermented products on proximate composition, free sugar, organic acid, 𝛽-glucan, ergothioneine, ergosterol, and vitamin D2 levels, and 3T3-L1 preadipocyte cell growth were studied. The proximate composition analysis results showed that the crude fiber and crude fat content in FCLM was higher than that in FPLM, and the crude protein and soluble nitrogen content in FPLM was higher than that in FCLM. Free sugar analysis detected arabinose, glucose, and sucrose in both FCLM and FPLM, and the total free sugar content was high in FPLM. The organic acid content was lower in FCLM and FPLM compared to C. lanceolata and P. grandiflorus before fermentation. The 𝛽-glucan content was higher than that of L. edodes used as a control in both fermented products, FCLM and FPLM. The content of ergothioneine, an antioxidant, was higher in FCLM than in FPLM. Ergosterol content was highest in L. edodes which was used as a control, and the two fermented products showed similar content. Vitamin D2 was detected only in FCLM and FPLM, and FPLM (0.58±0.01 mg%) showed a higher vitamin D2 content than FCLM (0.47±0.01). FCLM and FPLM showed a higher level of cell viability for 3T3-L1 pre-adipocytes compared to non-fermented C. lanceolata and P. grandiflorus. In addition, FCLM and FPLM inhibited 3T3-L1 preadipocyte differentiation more than C. lanceolata and P. grandiflorus before fermentation, which may exert an anti-obesity effect.

주제어

표/그림 (14)

그림 Fig. 1. The manufacture process of herbal extracts fermented with Lentinula edodes mycelials.
표 Table 1. HPLC conditions for free sugars analysis
표 Table 2. HPLC conditions for organic acids analysis
표 Table 3. HPLC conditions for ergothioneine analysis
표 Table 4. HPLC conditions for ergosterol and vitamin D₂ analysis
표 Table 5. Yields and proximate compositions of fermented herbal extract fermented with Lentinula edodes mycelials (unit:%)
표 Table 6. The content of free sugars in herbal extracts fermented with Lentinula edodes mycelials (unit:%)
표 Table 7. The content of organic acids in herbal extracts fermented with Lentinula edodes mycelials (unit:%)
표 Table 8. The content of β-glucan in herbal extracts fermented with Lentinula edodes mycelials (unit:%)
표 Table 9. The content of ergothioneine in herbal extracts fermented with Lentinula edodes mycelials (unit:mg%)
표 Table 10. The content of ergosterol in herbal extracts fermented with Lentinula edodes mycelials (unit:mg%)
표 Table 11. The content of vitamin D2 in herbal extracts fermented with Lentinula edodes mycelials (unit:mg%)
그림 Fig. 2. The cell viability of herbal extracts fermented with Lentinula edodes mycelials on 3T3-L1 cells. ¹⁾Mean with different superscripts (a,b,c) are significantly different at p<0.05 in same concentrations by Duncan's multiple range test. LE: Lentinula edodes, CL: Codonopsis lanceolata, PG: Platycodon grandiflorus, FCLM: Fermented Codonopsis lanceolata by Lentinula edodes mycelials, FPLM: Fermented Platycodon grandiflorus by Lentinula edodes mycelials
그림 Fig. 3. Inhibitory effect of herbal extracts fermented with Lentinula edodes mycelials on the lipid accumulation in 3T3- L1 preadipocyte. ¹⁾Mean with different superscripts (a,b,c) are significantly different at p<0.05 in same concentrations by Duncan's multiple range test. MDI: Differentiation medium (DMEM containing 10% FBS, 0.5 mM isobutylmethylxanthine (IBMX), 1 μM dexamethasone, and 10 μg/mL insulin), LE: Lentinula edodes, CL: Codonopsis lanceolata, PG: Platycodon grandiflorus, FCLM: Fermented Codonopsis lanceolata by Lentinula edodes mycelials, FPLM: Fermented Platycodon grandiflorus by Lentinula edodes mycelials

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