[논문]기능성화장품소재로서 자색당근 초임계추출물의 항산화 및 항주름 효능 연구

김지수; 이지안

doi:10.22156/cs4smb.2021.11.12.236

기능성화장품소재로서 자색당근 초임계추출물의 항산화 및 항주름 효능 연구
A Study on Anti-oxidant and Anti-wrinkle Effect of Supercritical Fluid Extraction of Black Carrot as a Functional Cosmetic Material 원문보기

융합정보논문지 = Journal of Convergence for Information Technology, v.11 no.12, 2021년, pp.236 - 243

김지수 (서경대학교 일반대학원 미용예술학과) , 이지안 (서경대학교 미용예술대학 뷰티테라피&메이크업학과)

초록
AI-Helper

본 연구는 자색당근(black carrot, BC) 소재로 초임계추출물에 대해 DPPH radical 소거능, ABTS radical 소거능, 총 폴리페놀 함량 측정을 통해 항산화 활성, 인간의 정상 섬유아세포에서 콜라겐 생성과 MMP-1 발현을 조사하여 항주름 효능을 평가하고자 하였다. 그 결과 자색당근 초임계추출물 내 총 폴리페놀 함량 (9.037±1.123 mg GAE/g), DPPH, ABTS 분석 결과로 높은 항산화 효과를 확인하였다. NHDF 세포에서 초임계추출물 농도에 의한 세포독성은 거의 없었으며, 이때 추출물은 농도 의존적으로 MMP-1 단백질 및 MMP-1 mRNA 발현을 억제하고, 콜라겐 type I 유전자 mRNA 수준 증가에 의한 type I procollagen의 합성을 촉진하였다. 이상의 결과를 종합해 볼 때, 자색당근 초임계추출물은 우수한 항산화 효능과 친환경(solvent free)물질, 항노화 원료로서 화장품, 식품, 미용식품 등 다양한 산업분야에 있어 활용 가능성이 매우 높을 것으로 판단된다.

Abstract ▼ AI-Helper

The aim of this study was to evaluate the anti-oxidant and anti-wrinkle effect of the black carrot(BC) extracted by supercritical dioxide(SC-CO2). DPPH/ABTS radical scavenging and total polyphenol contents were measured to investigate the anti-oxidant activity of the BC supercritical extract. Collagen production and MMP-1 expression were assessed in normal human dermal fibroblasts(NHDF) for anti-wrinkle activity, The black carrot extract showed the highest total phenolic content(9.037±1.123 mg GAE/g extract) and the best antioxidant properties as shown by DPPH and ABTS assay. The supercritical fluid extracts of black carrot exhibited low toxicity to NHDF cells. In addition, the supercritical fluid extracts showed MMP-1 inhibition and type I pro-collagen synthesis inducing activities in a dose-dependent manner, respectively. Therefore, these results suggest that the black carrot is a potential source of high anti-oxidative, solvent-free and anti-aging material with promising applications in cosmetic, food, and beauty-food industries.

주제어

표/그림 (9)

표 Table 1. Operating condition of BC SC-CO₂ extraction
표 Table 2. COL1, MMP-1 and GAPDH primer
표 Table 3. Yield of black carrot SC-CO₂ extracts
표 Table 4. Total phenolic contents of black carrot SC-CO₂ extracts
그림 Fig. 1. DPPH RSA of black carrot SC-CO₂ extracts. Values are means mean±S.D. of triplicate determinations.
그림 Fig. 2. ABTS RSA of black carrot SC-CO₂ extracts. Values are means mean±S.D. of triplicate determinations.
그림 Fig. 3. Effect of black carrot SC-CO₂ extracts on NHDF cell viability. Values are means mean±S.D. of triplicate determinations. Different superscripts within a column indicated significant differences (*p<0.05, **p<0.01).
그림 Fig. 4. Effects of black carrot SC-CO₂ extracts on type I pro-collagen synthesis (A) and mRNA level (B). Values are mean±S.D. of triplicate determinations. Different superscripts within a column indicated significant differences (p<0.05, **p<0.01).
그림 Fig. 5. Effects of black carrot SC-CO₂ extracts on MMP-1 expression. Values are mean±S.D. of triplicate determinations. Different superscripts within a column indicated significant differences (p<0.05, **p<0.01).

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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