[논문]새로운 betanodavirus 재편성체(reassortant)의 어류 치어에 대한 병원성 분석

김영철; 정현도

doi:10.7847/jfp.2021.34.2.133

[국내논문] 새로운 betanodavirus 재편성체(reassortant)의 어류 치어에 대한 병원성 분석
Pathogenicity of new reassortant betanodaviruses to various juvenile fishes 원문보기

韓國魚病學會誌= Journal of fish pathology, v.34 no.2, 2021년, pp.133 - 140

김영철 (국립수산과학원 병리연구과) , 정현도 (부경대학교 수산생명의학과)

Abstract ▼ AI-Helper

With the recent isolation of a new betanodavirus in shellfish, Korean Shellfish Nervous Necrosis Virus (KSNNV), it has also been identified the reassortant KSNNV of two RNA segments, in which one segment is KSNNV genotype but the other one is known genotype. In this study, we confirmed that the ressortant KSNNVs obtained in previous screening study of our laboratory for betanodaviruses in shellfish were KS/RGNNV and RG/KSNNV type by performing two consecutive multiplex RT-PCR on each RNA1 and RNA2 segment (R1- and R2-discriminative multiplex two-step RT-PCR, respectively) to determine the genotype of each segment based on the size of amplicon. In the pathogenicity analysis, none of the reassortants induced specific external symptoms or mortality of VNN, but viruses of 2 × 104~105 copies/mg or more were detected at 14 days after injection (107 copies/fish) in brain tissues of 4 species except for crucian carp and common carp among the 6 species of juvenile fish used. In addition, the histopathological features of weak but distinct vacuole formation were also found in the brain of these infected fish, but no difference was found between the two reassortants KS/RGNNV-KG and RG/KSNNV-CM.

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표/그림 (4)

표 Table 1. Primers used in this study
그림 Fig. 1. Application of two different multiplex RT-PCR for the cultured KS/RGNNV-KG and RG/KSNNV-CM reassortants. Mismatched genotype between RNA1 and RNA2 segment of KS/RGNNV-KG (A), RG/KSNNV-CM (B) was confirmed by corresponding size of each amplicon.
그림 Fig. 2. Histopathology of the infected brain tissue of experimental fish (H&E). Multiple vacuolations (arrows) were observed under light microscope in brains collected from various species of juvenile fish infected with reassortant at 14 days post injection (dpi) at 25°C. KS/RGNNV-infected sevenband grouper (Epinephelus septemfasciatus) (A), rock bream (Oplegnathus fasciatus) (B), olive flounder (Paralichthys olivaceus) (C), mandarin fish (Siniperca scherzeri) (D). RG/KSNNV infected rock bream (E), olive flounder (F), mandarin fish (G). Scale bar = 100 μm.
표 Table 2. Virus copies in the brain of six fish species at 14 days after inoculation with KS/RGNNV-KG or RG/ KSNNV-CM at 25°C

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