본 연구에서는 저분자펩타이드로부터 유래되는 단백질을 확인하기 위해 갈색거저리의 유충, 번데기, 성충의 저분자 단백질체 분석을 수행하였다. 저분자 펩타이드 분석으로부터 유래된 54 단백질이 최종적으로 확인되었다. 확인된 단백질 중 성체에만 존재하는 단백질이 가장 높은 빈도로 존재하였고, 그 다음은 성체와 유충에 동시 존재하는 단백질이 높은 빈도로 탐색되었다. 그러나 번데기를 포함하는 그룹들은 모두 낮은 빈도로 감지되었다. 분석된 단백질에 orthologous classification의 결과에서 일반적 기능 예견만(general function prediction only) 보이는 단백질이 가장 높은 빈도로 조사되었다. 크로마틴 구조와 동적상태(chromatin structure and dynamics)에 연관된 단백질은 비교적 높은 빈도로 탐색되었다. 또한, 아미노산 수송과 물질대사(amino acid transport and metabolism) 및 탄수화물 수송 및 물질대사(carbohydrate transport and metabolism)와 연관된 단백질도 높은 빈도로 분석되었다. 그러나 뉴클레오타이드 수송 물질대사, 코엔자임 수송 및 물질대사, 세포외 구조, 모빌로좀(mobilome), 및 핵 구조와 연관된 단백질은 전혀 탐지되지 않았다. 따라서 크로마틴, 아미노산, 탄수화물 물질대사와 연관된 단백질들이 보다 쉽게 저분자 펩타이드로 전환되어 체액 중에 잔존될 수 있는 것으로 보이며, 이들 펩타이드들이 생리활성물질로써 기능을 수행할 수 있을 가능성이 높은 것으로 추정된다.
본 연구에서는 저분자펩타이드로부터 유래되는 단백질을 확인하기 위해 갈색거저리의 유충, 번데기, 성충의 저분자 단백질체 분석을 수행하였다. 저분자 펩타이드 분석으로부터 유래된 54 단백질이 최종적으로 확인되었다. 확인된 단백질 중 성체에만 존재하는 단백질이 가장 높은 빈도로 존재하였고, 그 다음은 성체와 유충에 동시 존재하는 단백질이 높은 빈도로 탐색되었다. 그러나 번데기를 포함하는 그룹들은 모두 낮은 빈도로 감지되었다. 분석된 단백질에 orthologous classification의 결과에서 일반적 기능 예견만(general function prediction only) 보이는 단백질이 가장 높은 빈도로 조사되었다. 크로마틴 구조와 동적상태(chromatin structure and dynamics)에 연관된 단백질은 비교적 높은 빈도로 탐색되었다. 또한, 아미노산 수송과 물질대사(amino acid transport and metabolism) 및 탄수화물 수송 및 물질대사(carbohydrate transport and metabolism)와 연관된 단백질도 높은 빈도로 분석되었다. 그러나 뉴클레오타이드 수송 물질대사, 코엔자임 수송 및 물질대사, 세포외 구조, 모빌로좀(mobilome), 및 핵 구조와 연관된 단백질은 전혀 탐지되지 않았다. 따라서 크로마틴, 아미노산, 탄수화물 물질대사와 연관된 단백질들이 보다 쉽게 저분자 펩타이드로 전환되어 체액 중에 잔존될 수 있는 것으로 보이며, 이들 펩타이드들이 생리활성물질로써 기능을 수행할 수 있을 가능성이 높은 것으로 추정된다.
In this study, we examined low molecular weight peptides using proteomics in order to identify their original proteins, derive their peptides, and determine the functions of the proteins in Tenebrio molitor, the mealworm (larvae, pupae, or adult) from which the peptides were extracted. Fifty-four pr...
In this study, we examined low molecular weight peptides using proteomics in order to identify their original proteins, derive their peptides, and determine the functions of the proteins in Tenebrio molitor, the mealworm (larvae, pupae, or adult) from which the peptides were extracted. Fifty-four proteins were finally identified through an analysis of proteome to derive the analyzed peptides. The proteins that induced low molecular weight peptides were identified to be the most abundant in adults only, and the next highest were derived from a group containing both adults and larva. However, other groups, including pupa, were detected to have a lower frequency of peptides. As a result of orthologous classification of the detected proteins, the general function prediction was only investigated at the highest frequency among the examined proteins. Proteins related to chromatin structure and dynamics were detected by their higher frequency among functional classes. The next highest frequency was shown by proteins related to amino acid transport and metabolism and carbohydrate transport and metabolism. Therefore, it is assumed that proteins correlated with chromatin, amino acid, and carbohydrate metabolisms are easily induced into low molecular weight peptides, and that their peptides could play a role as bioactive substances.
In this study, we examined low molecular weight peptides using proteomics in order to identify their original proteins, derive their peptides, and determine the functions of the proteins in Tenebrio molitor, the mealworm (larvae, pupae, or adult) from which the peptides were extracted. Fifty-four proteins were finally identified through an analysis of proteome to derive the analyzed peptides. The proteins that induced low molecular weight peptides were identified to be the most abundant in adults only, and the next highest were derived from a group containing both adults and larva. However, other groups, including pupa, were detected to have a lower frequency of peptides. As a result of orthologous classification of the detected proteins, the general function prediction was only investigated at the highest frequency among the examined proteins. Proteins related to chromatin structure and dynamics were detected by their higher frequency among functional classes. The next highest frequency was shown by proteins related to amino acid transport and metabolism and carbohydrate transport and metabolism. Therefore, it is assumed that proteins correlated with chromatin, amino acid, and carbohydrate metabolisms are easily induced into low molecular weight peptides, and that their peptides could play a role as bioactive substances.
Proteins to be identified in groups including pupa showed totally low de-tection ratio. From the results, it is assumed that adult has the most low molecular weight peptides and the next larva has higher, but pupa maintains the lowest value. In fact, as a result of analysis in this laboratory, it was found that peptides less than 10 kDa possess various antibacterial activities (unpublished data).
From the results, it is assumed that adult has the most low molecular weight peptides and the next larva has higher, but pupa maintains the lowest value. In fact, as a result of analysis in this laboratory, it was found that peptides less than 10 kDa possess various antibacterial activities (unpublished data). Therefore, it is possible that the peptides shown in the current analysis may be involved in various physiological activities including innate immunity.
후속연구
In fact, as a result of analysis in this laboratory, it was found that peptides less than 10 kDa possess various antibacterial activities (unpublished data). Therefore, it is possible that the peptides shown in the current analysis may be involved in various physiological activities including innate immunity.
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