[논문]고지방식이 유도 비만 마우스에서 황련, 단삼, 육계 복합추출물의 비만 개선 및 당뇨 예방 효과

정수민; 권세은; 강석용; 김수진; 정효원; 박용기

doi:10.15429/jkomor.2021.21.2.59

고지방식이 유도 비만 마우스에서 황련, 단삼, 육계 복합추출물의 비만 개선 및 당뇨 예방 효과
Anti-Obesity and Anti-Diabetic Effects of a Polyherbal Extract Consisting of Coptidis Rhizoma, Salviae Miltiorrhizae Radix, and Cinnamomi Cortex in High Fat Diet-Induced Obesity Mice 원문보기

한방비만학회지 = Journal of Korean Medicine for Obesity Research, v.21 no.2, 2021년, pp.59 - 68

정수민 (동국대학교 한의과대학 본초학교실) , 권세은 (동국대학교 한의과대학 본초학교실) , 강석용 (동국대학교 한방신약개발센터) , 김수진 (동국대학교 의과대학 경주병원 마취통증의학교실) , 정효원 (동국대학교 한의과대학 본초학교실) , 박용기 (동국대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Objectives: We investigated the effects of Clean-DM4 (C-DM4), a polyherbal extract consisting of Coptidis Rhizoma, Salviae Miltiorrhiza Radix, and Cinnamomi Cortex on high fat diet (HFD)-induced obesity and diabetes in mice. Methods: The C57BL/6 mice (6 weeks) were fed a HFD for 8 weeks and then administrated with C-DM4 extract at 500 mg/kg (p.o.) once daily for 4 weeks. The changes of body weights, calorie intakes, and fasting blood glucose (FBG) levels were measured in mice. The serum levels of glucose, insulin, total cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured in mice by enzyme-based assay. It was also observed the histological changes of pancreas, liver, and fat tissues with hematoxylin and eosin (H&E) staining. Results: The increase of calorie intakes and FBG levels in HFD-induced obesity mice was significantly decreased by oral administration of C-DM4 extract. C-DM4 extract administration was significantly reduced the increased levels of glucose, insulin, total cholesterol, AST, and ALT in obesity mice. In addition, C-DM4 extract inhibited lipid droplet accumulation in liver tissues of obesity mice, hyperplasia of pancreatic islets, and enlargement of adipocytes in adipose tissues. Conclusions: Our study indicates that C-DM4 extract could help improve obesity and to prevent diabetes progression.

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표/그림 (6)

표 Table 1. Composition of C-DM4
그림 Fig. 1. Effects of C-DM4 extract on the increases of body weights and food intakes in high fat diet-induced obesity mice. (A) Body weights were measured in mice once a week during drug administration. (B) Calorie intake was calculated using food intakes at 9~12 week. Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, and ***P<0.001 vs. control (a) or HFD group (b). Cont: normal group, HFD: high fat diet-induced diabetic control group, C-DM4: C-DM4 at 500 mg/kg-administrated group, Met: metformin-administrated group.
그림 Fig. 2. Effects of C-DM4 extract on the levels of fasting blood glucose in high fat diet-induced obesity mice. The levels of FBG were measured in whole blood of mice once a week for 4 weeks. Data were presented as mean±standard deviation (n=5 per a group). **P<0.01, and ***P<0.001 vs. control (a) or HFD group (b). Cont: normal group, HFD: high fat diet-induced diabetic control group, C-DM4: C-DM4 at 500 mg/kg-administrated group, Met: metformin-administrated group, FBG: fasting blood glucose.
그림 Fig. 3. Effects of C-DM4 extract on the serum levels of serological markers in high fat diet-induced obesity mice. The levels of glucose (A), insulin (B), total cholesterol (C), AST (D), and ALT (E) were measured in the sera of mice after administration of drugs. Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, **P<0.01, and ***P<0.001 vs. control (a) or HFD group (b). Cont: normal group, HFD: high fat diet-induced diabetic control group, C-DM4: C-DM4 at 500 mg/kg-administrated group, Met: metformin-administrated group, AST: aspartate aminotransferase, ALT: alanine aminotransferase.
그림 Fig. 4. Effects of C-DM4 extract on the histological changes of liver and pancreas tissues in high fat diet-induced obesity mice. The liver (A) and pancreas (C) tissues were stained with hematoxylin and eosin and observed under microscope (x200). The weights of liver (B) and pancreas (D) were measured in mice at 12 week. Data were presented as mean±standard deviation (n=5 per a group). *P<0.05, **P<0.01, and ***P<0.001 vs. control (a) or HFD group (b). Cont: normal group, HFD: high fat diet-induced diabetic control group, C-DM4: C -DM4 at 5 00 m g/kg-administrated group, Met: metformin-administrated group.
그림 Fig. 5. Effects of C-DM4 extract on the histological changes of epididymal fat tissues in high fat diet-induced obesity mice. The fat tissues were stained with hematoxylin and eosin and observed under microscope (x200). The weights of fat were measured in mice a t 12 week. C ont: n ormal group, H FD: high f at d iet-induced diabetic control g roup, C-DM4: C -DM4 at 5 00 mg/kg-administrated group, Met: metformin-administrated group.

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